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The porcine sperm motility inhibitor is identical to β-microseminoprotein and is a competitive inhibitor of Na+, K+-ATPase.

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The porcine sperm motility inhibitor is

identical to β-microseminoprotein and is

a competitive inhibitor of Na+,

K+-ATPase

鄭海倫

Chao CF;Chiou ST;Jeng H and Chang WC

摘要

Abstract

We have isolated a small porcine seminal protein called SMI-1. Our results from peptide sequence, amino acid composition and mass spectral analyses reveal that SMI-1 is identical to porcine beta-microseminoprotein, a protein with unknown function. We also report here that this protein inhibits competitively the activity of Na+,K(+)-ATPase purified from porcine cerebral cortex in a dose-dependent manner. The inhibitory effect could be

reversed by the addition of ATP. The half-maximal inhibition was achieved at an inhibitor concentration of 90 microM.

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