Dietary glutamine supplementation reduces
cellular adhesion molecule expression and
tissue myeloperoxidase activity in mice with
gut-derived sepsis
許淳森
Yeh CL;Hsu CS;Yeh SL;Lin MT;Chen WJ
摘要Abstract
Objectives: This study investigated the effects of glutamine (Gin) on plasma intracellular adhesion molecule-1 levels and leukocyte integrin (CD 11a/CD18 and CD11b/CD18) expressions in gut-derived sepsis. Myeloperoxidase (MPO) activities in organs were also analyzed to identify the extent of tissue injury resulting from neutrophil infiltration. Methods: Mice were randomly assigned to a normal group (NC), a control group, or a Gin group. The NC group was fed standard chow diet; the control group was fed a common semipurified diet; and the Gin group received a diet in which part of the casein was replaced by Gin, which provided 25% of total amino acid nitrogen. After 3 wk, sepsis was induced by cecal ligation and puncture (CLP) in the control and Gin groups. Mice in the experimental groups were killed at 0, 6, 12, and 24 h after CLP. Mice in the NC group were killed when CLP was performed. Blood and organ samples were collected for further analysis. Results: Plasma intracellular adhesion molecule-1 levels were significantly lower in the Gin group than in the control group at 6, 12, and 24 h after CLP. Expressions of lymphocyte CD11a/CD18 were significantly higher, whereas polymorphonuclear
lymphocyte expressions of CD11b/CD18 were lower in the Gin group than in the corresponding control group at 6 and 12 h after CLP. In comparisons of MPO activities in various organs, the Gin group had lower MPO activities at 6 and 12 h in the lung, at 6, 12, and 24 h in the liver, at 12 and 24 h in the kidneys , and at 12 h in the intestine than those in the control group. Conclusions: Results of this study demonstrate that a Gin- supplemented enteral diet increased lymphocyte CD11a/CD 18 expressions, whereas neutrophil CD11b/ CD18 expressions, circulating intracellular adhesion
molecule-1 levels, and MPO activities in various organs decreased with gut-derived sepsis. These findings suggest that, under septic conditions , Gin administration may enhance lymphocyte function, attenuate interactions between polymorphonuclear lymphocytes and endothelium, and thus may decrease neutrophil infiltration into tissues. (c) 2006 Elsevier Inc. All rights reserved.
