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Interaction of cyclin D1 genotype and smoking habit in lung cancer
Fang-Jing Li2,3, Wen-Shin Chang2,4, Chia-Wen Tsai2,4, Meng-Hsuan Lee2,5, Te-Chun Hsia1,2*, Chin-Jung Liu1,3, Hong-Zin Lee6, Hau-Yang Ho7, Song-Shei Lin7,and Da-Tian Bau2,4,5*
Corresponding Email: [email protected]
1
Department of Pulmonary and Critical Care Medicine,
2
Terry Fox Cancer Research Laboratory, China Medical University Hospital,
3
Department of Respiratory Therapy, 4Graduate Institute of Basic Medical Science,
5
Graduate Institute of Clinical Medical Science, 6School of Pharmacy, China Medical University
7
Department of Medical Imaging and Radiological Sciences, Central-Taiwan University of Science and Technology
Keywords: Cyclin D1, lung cancer, carcinogenesis, genotype
The cyclin D1 (CCND1) is critical in the transition of cell cycle from G1 to S phase and unbalanced
cell cycle regulation is a hallmarker of carcinogenesis. The study aimed at investigating the association of
CCND1 genotypes with lung cancer risk in Taiwan and examining the gene-environment interaction
among CCND1 genotype and smoking habits. The genotype of CCND1 A870G (rs9344) and C1722G
(rs678653) were determined by polymerase chain reaction (PCR) and restriction fragment length
polymorphism (RFLP) analysis of DNA from the blood. The study recruited 358 lung cancer patients and
716 cancer free health controls. The results showed that there were significant differences between lung
cancer and control groups in the distribution of the genotypes (P=0.0003) and allelic frequency
(P=0.0007) in the CCND1 rs9344 genotype. Individuals who carried AG or GG genotype had 0.59- and
0.52-fold of odds ratio of developing lung cancer compared to those who carried the AA genotype
(95%CI=0.44-0.78 and 0.35-0.79, respectively). There was also an obvious interaction of CCND1 rs9344
genotype with personal smoking habit on lung cancer risk (P=0.0009). These findings support the
conclusion that the cell cycle regulation may play a role in lung cancer development and that CCND1
