• 沒有找到結果。

Conjunctival Epithelial Cells in Culture-growth and Goblet Cell Differentiation

N/A
N/A
Protected

Academic year: 2021

Share "Conjunctival Epithelial Cells in Culture-growth and Goblet Cell Differentiation"

Copied!
1
0
0

加載中.... (立即查看全文)

全文

(1)

Conjunctival Epithelial Cells in

Culture-growth and Goblet Cell

Differentiation

蔡瑞芳

Tsai RJF;Tseng SCG;Chen JK

摘要

Abstract

Conjunctival epithelial cell growth and differentiation were studied by

cultivating cells on tissue culture plastic surface and on natural substrata such as collagen gel and matrigelR. Well-differentiated goblet cells were unable to attach in plastic cultures and could only be preserved in collagen gel- or matrigelR - based cultures. Percoll density fractionation experimental suggested that, in the primary conjunctival epithelial cells, there were precursor cells for goblet and non-goblet epithelial cells. The goblet cell phenotypic expression of these precursor cells was influenced by the surface to which they attach and by the serum factors. The PAS and AM-1 positive cells could also be induced when the precursor cells are cultured on collagen gels in serum-free define medium supplemented with retinoic acid. To study how the goblet cell precursors are differentiated and from what stem cells they are derived, it is necessary to develop a culture system with a better mimicry of the in vivo conjunctival tissue. In this regard, wedeveloped an in vitro

‘conjunctival equivalent’, in which the epithelial cells were cultured on

fibroblast-contracted collagen lattice to allow continued cross-interactions of the epithelial and mesenchymal cells. This experimental model should allow experimental inquiries that are difficult, if not impossible, in conventional cell cultures.

參考文獻

相關文件

Giant cells are also seen in other diseases like giant cell granuloma of the jaws, traumatic bone cyst, aneurysmal bone cyst, and jaw tumor of

From the left, epidermal disarray, plentiful small and bright cells, and scattered larger dendritic cells are apparent in the suprabasal epithelium, indicating likely LM (C), then a

PDLSCs: Periodontal Ligament derived Stem Cells GMSCs: Gingiva derived Mesenchymal Stem Cells SHED: Stem cells from Human Exfoliated Deciduous teeth IDPSCs: Immature Dental Pulp

They could also successfully form single-cell derived colonies (Fig.. Pyogenic granuloma-derived cells. A) After the initial seeding, many cells attached to the plates in an

Higher immunoexpression of HIF-1 a, NOTCH1, ADAM-12, and heparin-binding epidermal growth factor like growth factor (HB-EGF) in epidermoid cells in compari- son with mucous cells

(1. International arts organization: A foundation, association, company, group, or unit whose mission is geared toward culture or the arts. Examples include the American Theatre

The main interest in the interpretation and discussion of passages from the sutra is to get a clear picture of how women are portrayed in the sutra and to find out

• to develop a culture of learning to learn through self-evaluation and self-improvement, and to develop a research culture for improving the quality of learning and teaching