The effects and mechanisms of Flemingia macrophylla on anti-photoaging Kuo-Ching Wen, Sue-Tsai Liao, Pei-Ching Fan, Hsiu-Mei Chiang
Department of Cosmeceutics, China Medical University, Taichung, Taiwan 404 E-mail of corresponding author: [email protected]
Key words: Flemingia macrophylla, MMP, typeⅠprocollagen, photoaging, Objectives
Matrix metalloproteinase-1, 3, 9 (MMP-1, 3, 9) play important roles in photoaging. The production of MMP-1, 3, 9 in skin is increased after UV-irradiation. The increase then causes the degradation of collagen and elastin even forms coarse wrinkles and sagging skin. Skin photocarcinogenecity also causes by UV-induced ROS, which can initiate lipid peroxidation and produce lipid alkoxy radicals, then combine with DNA to form DNA adducts.
Polyphenols and flavonoids possessed a variety of biological activities including anti-oxidation and free radical scavenging effects. According to the literatures, many polyphenols and flavonoids had significant inhibitory effects on matrix metalloproteinase 1, 3 or 9 in dermal fibroblast and chemoprotective activity for skin cancer. Flavonoids are widely distributed in natural plants of Fabaceae. In our preliminary test, Flemingia macrophylla extract showed marked inhibition of collagenase and antioxidant activities in a dose dependent manner.
Materials and Methods
The water extract of Flemingia macrophylla was subjected to the antioxidant and antiphotoaging studies. The antioxidant effect was examined by DPPH scavenging and AAPH-induced haemolysis assay. The effects and mechanisms of the extract was investigated by MMPs activity assays by fluorescent gelatin, elastase assay, UVB irradiation, gelatin zymography, typeⅠprocollagen assay and MTT assay in human fibroblasts (Hs68) after UV exposure.
Results
The water extraction yield of Flemingia macrophylla was 18.2%. The result of gelatin digestion assay showed that Flemingia macrophylla extract inhibited MMP-1 activity at 100 g/mL. In fluorescence-substrate assay, the inhibition of Flemingia macrophylla extract on MMP-1 activity showed a dose-dependent manner within 10, 50, 100, 500 g/mL. Furthermore, the Flemingia macrophylla extract for the DPPH radical-scavenging activity at the concentration of 5 μg/mL was similar to ascorbic acid (50 μg/mL). The Flemingia macrophylla extract also possessed inhibitory activity against AAPH-induced haemolysis of erythrocytes in dose- and time-dependent manner at concentrations of 50 to 500 g/mL from 1 to 4 h. The results shown that pretreated with Flemingia macrophylla extract at the concentration of 10 g/mL could decrease expression of MMP-1, -3 and -9 respectively. In addition, the Flemingia macrophylla extract has no cytotoxicity.
Conclusion
These findings shown that Flemingia macrophylla extract exhibited antioxidant activity resulting in inhibition of MMP-1, -3 and -9. They could be potential a
cosmeceutical material to improve wrinkle of intrinsic aging and photoaging skin. Reference
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