Cruciferous vegetable derivatives on the activation of RAW 264.7 cells secretion of inflammatory mediators
Effects of cruciferous vegetable derivatives on inflammatory mediators secreted by activated RAW 264.7 cells
Abstract
This study used mouse macrophage RAW 264.7cell line as experimental model to investigate the cruciferous vegetable derivatives in β-phenylethyl
isothiocyanate (PEITC) and indole-3-carbinol (I3C) for the inflammation associated with LPSinduced an oxidation factor nitrogen (nitric oxide, NO), tumor necrosis factor (tumor necrosis factor-α, TNF-α), interleukin-1β (interleukin-1β, IL-1β), interleukin-10 (IL-10), prostaglandin (prostaglandinE2, PGE2) generated effects, and to further understand the possible mechanism. The results showed that addition of PEITC (5x10-7 M ~ 10-5 M) and I3C (10-5 M ~ 10-4 M) inhibited the LPS inducedNO generation and the dose effect, in which the effect was more PEITC obvious. The higher concentration of PEITCinhibited the LPS induced by the different cytokine TNF-α, IL-10and PGE2generation; and I3C could inhibit the LPS inducedTNF-α, IL-10 generation, but for PGE2and IL-1β generation.
Western blot analysis revealed that high concentrations of PEITCand I3C could promote cell COX-2 (cyclooxgenase-2) protein expression. In addition, PEITC and I3C has a direct role of NOremoval. The above results, cruciferous vegetable derivatives PEITCand I3C on the LPS induced NO, PGE2, and cytokine generation have different functions, which PEITC could inhibit theLPS induced NO, TNF-α, IL- 10 and PGE2generation; I3C could inhibit theLPS induced NO, TNF-α and IL-10 generation, but to promote theformation of IL-1β and LPScan be induced by the promotion of COX-2 protein expression, increased PGE2generation. On the other hand, PEITC and I3C, the role ofNO through direct removal, while the lower medium concentration of NO.
English Abstract
