2012 食品營養細胞學實驗

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2012 食品營養細胞學實驗

Date Topics

02/14 Introduction

1. Outline of Cell Biology Experiments 2. Equipments of Cell Biology Experiments 3. Sterile Handling

02/21 1 Preparation of Medium 1.1 Content of Medium 1.2 Aseptic Technique 1.3 Sterile Filtration

2 Cell Culture and Growth Curve

02/28 和平紀念日放假一日

03/06 Detection of Chromosome Content

03/13 Fluorescent Staining of Lysomes and Mitochondria 03/20 SDS Polyacrylamide Gel Electrophoresis

(Sample and PAGE preparation)

03/27 SDS polyacrylamide Gel Electrophoresis (Sample loading、stained and distain)

04/03 春節放假一日

04/10 實驗數據與分析(上台報告)

04/17 實驗期中考

04/24 Assay of Cell Viability: MTT Test and Cell Freezing 05/01 Measurement of Intracellular calcium Levels and Cell

Thawing

05/08 RNA Analysis : Isolation and Electrophoresis of RNA 05/15 Flow cytometry

05/22 實驗數據與分析(上台報告)

05/29 實驗操作考

06/05 Check Out &實驗期末考

數據

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參考文獻

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