2012 食品營養細胞學實驗
Date Topics
02/14 Introduction
1. Outline of Cell Biology Experiments 2. Equipments of Cell Biology Experiments 3. Sterile Handling
02/21 1 Preparation of Medium 1.1 Content of Medium 1.2 Aseptic Technique 1.3 Sterile Filtration
2 Cell Culture and Growth Curve
02/28 和平紀念日放假一日
03/06 Detection of Chromosome Content
03/13 Fluorescent Staining of Lysomes and Mitochondria 03/20 SDS Polyacrylamide Gel Electrophoresis
(Sample and PAGE preparation)
03/27 SDS polyacrylamide Gel Electrophoresis (Sample loading、stained and distain)
04/03 春節放假一日
04/10 實驗數據與分析(上台報告)
04/17 實驗期中考
04/24 Assay of Cell Viability: MTT Test and Cell Freezing 05/01 Measurement of Intracellular calcium Levels and Cell
Thawing
05/08 RNA Analysis : Isolation and Electrophoresis of RNA 05/15 Flow cytometry
05/22 實驗數據與分析(上台報告)
05/29 實驗操作考
06/05 Check Out &實驗期末考
