花草茶水萃取液的抗氧化活性分析 蕭惠茹、吳芳禎
E-mail: [email protected]
摘 要
本研究以薰衣草、紫羅蘭、迷迭香、檸檬草、檸檬馬鞭草、金盞花、薄荷葉、洋甘菊、矢車菊、菩提葉、鼠尾草和香蜂葉 等十二種之市售花草茶為材料，利用清除DPPH(α-diphenyl-β-picrylhydrazyl)自由基能力、螯合亞鐵離子能力和還原力等 三種方法分析其水萃取液抗氧化活性。並測定萃取液中總酚的含量，同時和0.02％ BHT(Butylated hydroxytoluene)之抗氧化 性作比較。 實驗結果顯示，在DPPH自由基清除能力方面，當檸檬馬鞭草(70 ℃)萃取液達40 mg/mL時，其清除率達90.03
％，效果可以和0.02％ BHT相比。無論是50 ℃或70 ℃的萃取液，檸檬馬鞭草、迷迭香、香蜂葉和鼠尾草，其DPPH自由 基清除率都可達60％。50 ℃和70 ℃花草茶水萃取液螯合鐵離子之能力皆不及0.02％ BHT。整體表現以薰衣草水萃取液最 穩定，在不同溫度萃取及在不同濃度下都有60％螯合鐵離子的能力，結果顯示並非濃度增加而螯合能力有增強效果。檸檬 馬鞭草和薄荷葉，70 ℃的水萃取液比50 ℃萃取液有明顯較高的螯合鐵離子的能力。而菩提葉、金盞花、矢車菊和紫羅蘭
，在70 ℃水萃取液比50 ℃水萃取液的螯合鐵離子能力反而低。還原力測定方面，70 ℃萃取液當濃度提升到 20 mg/mL時
，迷迭香、菩提葉、薄荷葉、金盞花的還原力隨著濃度則有顯著性差異(P0.05)。而50 ℃水萃取液，除了菩提葉、鼠尾草、
金盞花、紫羅蘭及薰衣草以外，其餘六株花草茶總酚結果也顯示無顯著性差異(P>0.05)。
關鍵詞 : 抗氧化活性、螯合亞鐵離子、還原力、總酚類、花草茶 目錄
封面內頁 簽名頁 授權書... iii 中文摘要... iv 英文摘要... vi 誌謝... viii 目錄... ix 圖目錄... xi 表目
錄... xii 第一章 緒言... 1 第二章 文獻回顧... 3 第一節 花草 茶... 3 ㄧ、簡介... 3 二、醫療上的應用... 12 第二節 抗氧化劑的起 源... 18 ㄧ、天然抗氧化物的發展... 18 二、天然抗氧化物的種類... 20 三、抗氧化劑 作用機制... 23 四、人工抗氧化劑的種類... 24 （一） Butylated hydroxyanisole (BHA)... 24 （ 二） Butylated hydroxytoluene (BHT)... 25 （三） Tertiary butylhydroquinone(TBHQ)... 25 （四） Esters of gallic acid (PG)... 25 第三節 人體中存在抗氧化物質... 26 第四節 抗氧化活性測定法之原理... 27 第三章 材料 與方法... 30 第一節 材料... 30 ㄧ、花草茶... 30 二、藥
品... 30 三、儀器... 31 第二節 方法... 31 ㄧ、萃取液的製 備... 31 二、抗氧化活性分析... 32 （一）DPPH自由基清除能力測定... 32 （二）
亞鐵離子螯合能力測定... 32 （三）還原力的測定... 33 三、抗氧化活性成分之定量分 析... 33 （一）總酚類含量測定... 33 第四章 結果與討論... 34 第五章 結 論... 48 參考文獻... 52
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