• 沒有找到結果。

m has been studied in the blackfoot disease endemic area. The study subjects who were over 30 years old a

N/A
N/A
Protected

Academic year: 2021

Share "m has been studied in the blackfoot disease endemic area. The study subjects who were over 30 years old a"

Copied!
2
0
0

加載中.... (立即查看全文)

全文

(1)

台灣烏腳病盛行地區高血壓與含錳超氧歧化酵素基因多形性之相關性研 究

針對烏腳病盛行地區探討含錳超氧歧化酵素( MnSOD )基因多形性和高血壓之間的關 係。以 30 歲以上並且每週居住在研究地區至少五天的居民作為研究對象,並利用 78 年 收集的資料以及 78 到 86 年間所採集的生物檢體。兩位公共衛生護士以結構式問卷訪視 每位研究對象,問卷內容包括社會人口學資料、抽菸與喝酒習慣、職業史、居住史、飲 水史與家族疾病史等項目。血球萃取出之 DNA 利用聚合酵素連鎖反應及限制片段長度 多形性分析含錳超氧歧化酵素粒線體目標序列 Val/Ala 變異。利用高效率液相層析儀分 析血清中微量營養元素包括維生素 A 、維生素 E 、蕃茄紅素、 B— 胡蘿蔔素。調整高 血壓傳統危險因子及累積砷暴露後,含錳超氧歧化酵素 Val/Ala 和 Ala/Ala 基因型者,

高血壓相對危險性是 Val/Val 基因型者的 2 倍,顯著偏高。調整年齡、性別、身體質量 指數、累積砷暴露等重要危險子後,含錳超氧歧化酵素基因型為 Val/Ala 和 Ala/Ala 者 不論三酸甘油脂及維生素 E 濃度為何,危險對比值有上升趨勢。調整了年齡和性別後

,帶有 Val/Val 基因型且累積砷暴露越低、身體質量指數正常、三酸甘油脂濃度越低者

,高血壓的相對危險性有下降的趨勢。調整重要危險因子後,含錳超氧歧化酵素基因型 為 Val/Val 時,累積砷暴露在 10.2 ppm* 年以上者高血壓相對危險性是基因型為 Val/Val 時,累積砷暴露小於 10.2 ppm* 年者的 7.9 倍,呈統計上顯著性,此外,也發現累積砷 暴露在 10.2 ppm* 年以上者,不論三酸甘油脂、低密度脂蛋白膽固醇及維生素 E 濃度為 何,高血壓的相對危險性有上升的趨勢。因此本研究推論含錳超氧歧化酵素基因多形性 可能是高血壓基因上的一個易感受性因子,基因型的不同可能修飾個體罹患高血壓的危 險性,而後天環境的暴露、生活及飲食習慣更是影響高血壓相當重要的危險因子。

(2)

The Relationship between Manganese Superoxide Dismutase Gene P olymorphism and Hypertension in Blackfoot Disease Hyperendemic Ar

ea in Taiwan

The relationship between hypertension and manganese superoxide dismutase (MnSOD) gene polymorphis

m has been studied in the blackfoot disease endemic area. The study subjects who were over 30 years old a

nd lived at least 5 days a week in the three villages were recruited. The data of study subjects were collecte

d in 1989 and biological samples were obtained from 1989 to 1997. Two well-trained public health nurses

performed a standardized personal interview based on a structured questionnaire. Information obtained fro

m the interview included demographic characteristics, alcohol drinking and cigarette smoking, working an

d residential history, history of well water consumption as well as family history of hypertension. Moreove

r, DNA was extracted from buffy coat to analyze the Val/Ala variant in the mitochondrial targeting sequen

ce in MnSOD gene utilizing polymerase chain reaction (PCR) and restriction fragment length polymorphis

m (RFLP). Serum was measured for all trans-retinol, a-tocopherol, lycopene,B-carotene by high performan

ce liquid chromatography (HPLC).The relative risk of MnSOD Val/Ala and Ala/Ala genotype was twice in

crement than the MnSOD Val/Val genotype after adjusting the traditional risk factors of hypertension and

cumulative arsenic exposure. After adjustment for age, sex, body mass index (BMI) and cumulative arsenic

exposure, MnSOD Val/Ala and Ala/Ala genotype people had higher relative risk than MnSOD Val/Val gen

otype people in any concentration of triglyceride and vitamin E. The relative risk of hypertension for MnS

OD Val/Val genotype people in lower cumulative arsenic exposure, normal BMI and lower triglyceride lev

el was decreased after age and sex were adjusted. The odds ratios of MnSOD Val/Val genotype and cumula

tive arsenic exposure over 10.2 ppm*year was 7.9, and significantly higher than MnSOD Val/Val genotype

and cumulative arsenic exposure lower than 10.2 ppm*year after adjusting the important risk factors. Besid

es, whether lower triglyceride, LDL-cholesterol and vitamin E concentration, the relative risk of hypertensi

on was increasing in the case of people with cumulative arsenic exposure over 10.2 ppm*year. The results

of this study suggested that MnSOD gene polymorphism may be a genetic susceptible factor of hypertensi

on and MnSOD genotype may modify individual hypertension risk. Acquired environment exposure, life st

yle and dietary habit were also very important risk factors for hypertension.

參考文獻

相關文件

By 1905, “Coke" was being advertised all over the country as “The Great Natural Temperance Drink." The drink enjoyed additional success since there was a large and

Animal or vegetable fats and oils and their fractiors, boiled, oxidised, dehydrated, sulphurised, blown, polymerised by heat in vacuum or in inert gas or otherwise chemically

Milk and cream, in powder, granule or other solid form, of a fat content, by weight, exceeding 1.5%, not containing added sugar or other sweetening matter.

In taking up the study of disease, you leave the exact and certain for the inexact and doubtful and enter a realm in which to a great extent the certainties are replaced

In taking up the study of disease, you leave the exact and certain for the inexact and doubtful and enter a realm in which to a great extent the certainties are replaced

In 2006, most School Heads perceived that the NET’s role as primarily to collaborate with the local English teachers, act as an English language resource for students,

Research has suggested that owning a pet is linked with a reduced risk of heart disease, fewer visits to the doctor, and a lower risk of asthma and allergies in young

Research findings from the 1980s and 90s reported that people who drank coffee had a higher risk of heart disease.. Coffee also has been associated with an increased risk of