• 系統編號 RC9209-0002
• 計畫中文名稱 探討 Thy-1 在血管增生過程中所扮演的角色(III)
• 計畫英文名稱 Studies of Functional Roles of Thy-1 during Angiogenesis (III)
• 主管機關 行政院國家科學委員會 • 計畫編號 NSC90-2320-B038-032
• 執行機構 台北醫學院醫學研究所
• 本期期間 9008 ~ 9107
• 報告頁數 7 頁 • 使用語言 英文
• 研究人員 李文森 Lee, Wen-Sen
• 中文關鍵字 Thy-1 醣蛋白；血管新生；轉染；人類臍靜脈內皮細胞
• 英文關鍵字 Thy-1；Angiogenesis；Transfection；Human umbilical vein endothelial cell (HUVEC)
• 中文摘要 查無中文摘要
• 英文摘要
Transfection of cDNA into human primary cell cultures is always a big challenge for the laboratory. In the present study, we have succeeded in the human Thy-1 cDNA construct and transfection of this cDNA into HUVEC and NIH3T3 cells. The expression of human Thy-1 protein in the transfected HUVEC and NIH3T3 was demonstrated by Western blot analysis. The transfection of human Thy-1 cDNA into NIH3T3 cells
increased cell growth rate. Although our study of the Thy-1 transfection effect on the HUVEC is still on going, the finding from the human Thy-1 transfected NIH3T3 cells was consistant with our previous findings showing that using the anti-Thy-1 antibody which activates Thy-1
dose-dependently increased thymidine incorporation, and using the anti-Thy-1 antisense oligonucleotide to neutralize the Thy-1 expression dose-dependently decreased thymidine incorporation. Taken together, the results from these series studies suggest that Thy-1 protein, which is expressed in the angiogenic microvascular endothelial cells but not normal resting endothelial cells, might participate in the activating of the process of angiogenesis
