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幹細胞與再生醫學中心 Mechanical phenotype of cancer cells: cell softening and loss stiffness sensing

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國立成功大學「邁向頂尖大學計畫」

  延攬優秀人才工作報告表

NCKU’s “Aim for the Top University Project”

Work Report Form for Distinguished Scholars □續聘continuation of employment ■離職resignation

100 年 7 月 13 日更新 受聘者姓名

Name of the Employee 蔡世傑 ■

男 女 Male Female 聘 期 Period of Employment from 106 年(y) 9 月(m) 5 日(d) to 106 年(y) 12 月(m) 31 日(d) 研究或教學或科技研發與 管理計畫名稱 The project title of research,

teaching, technology development and management

幹細胞與再生醫學中心 Mechanical phenotype of cancer cells: cell softening and loss stiffness sensing 計畫主持人 (申請單位主管) Project Investigator (Head of Department/Center) 湯銘哲 補助延聘編號

Grant Number HUA - - -

一、 研究、教學、科技研發與管理工作全程經過概述。(由受聘人填寫)

Please summarize the entire research, teaching, or science and technology R&D and management work process (To be

completed by the employee)

Osteogenesis imperfecta (OI) is a group of genetic disorders that mainly affect the bones.

The severity may be mild to severe. The mechanism of OI is usually related to mutations in the COL1A1 or COL1A2 genes. There is no efficient treatment of OI so far and the best solution for OI patient ismaintenance of healthy lifestyle.Treatment may include care of broken bones, pain medication, physical therapy, or the support of medications of the bisphosphonate salt. To understanding the mechanism of OI, the SD rats carrying COL1A1 or COL1A2 mutations created by GeColl biomedical company (Taiwan) are used as the model. Dr. Tsai was responsible for the breeding of COL1A1 or COL1A2 mutant SD rats as well as the protocol of genotyping. Due to the effect of mutation on COL1A1 and COL1A2 gene, the rat may limp with teeth dysplasia. The pups must be injected with 3mg zoledronic acid per 100g body weight 14 days after birth and monitored carefully. The rats are supplied with hydro gel and rinsed feed to reduce the adverse effect of limp and teeth dysplasia. The breeding strategy at current stage is to cross mutant rats with wild type rat to expand the colony. At present one litter of COL1A1 mutant rats (mt/wt) and one litter of COL1A2 mutant rats (mt/wt) are obtained.

*Due to confidentiality agreement with GeColl biomedical company, the detail of COL1A1 and COL1A2 mutant as well as genotyping protocol are not described in the report.

Figure 1. Genotyping of COL1A1 and COL1A2 mutant rat. The designed primer pair

recognize COL1A1 mutant DNA only with a 1723 bp amplicon. The primer pair recognize COL1A2 mutant DNA only with a 1767 bp amplicon. At present 6 pups of COL1A1 mt/wt and 5 pups of COL1A2 mt/wt are obtained. Pups are conducted to genotyping examination 10 days after birth. Pups carry mutant gene are then injected with zoledronic acid 14 days after birth.

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二、研究或教學或科技研發與管理成效評估(由計畫主持人或單位主管填寫)

Please evaluate the performance of research, teaching or science and technology R&D and management Work: (To be completed

by Project Investigator or Head of Department/Center)

(1)是否達到延攬預期目標?

Has the expected goal of recruitment been achieved?

是的。

(2)研究或教學或科技研發與管理的方法、專業知識及進度如何?

What are the methods, professional knowledge, and progress of the research, teaching, or R&D and management work?

受聘者蔡世傑博士主動參與本實驗室的許多研究主題,包含生物醫學與生物力學跨領域的 合作。並協助指導實 驗是學生疾病模式鼠之專業照護飼養觀念及方法,使本實驗室在動物實驗方面,兼顧研究專業與動物福祉。 (3)受延攬人之研究或教學或科技研發與管理成果對該計畫(或貴單位)助益如何?

How have the research, teaching, or R&D and management results of the employed person given benefit to the project (or your unit)? 聘者蔡世傑博士對本實驗室的研究發展有很大的貢獻。本實驗室與基可生醫公司合作,引進該公司產製之 OI 疾病模式鼠, 該模式鼠在 8 周內即可能發病。蔡博士以專業動物飼育經驗,管理該品系大鼠之繁殖,並改良基因鑑定之引子設計,使該品 系大鼠可順利繁殖第一代仔鼠。

(4)受延攬人於補助期間對貴單位或國內相關學術科技領域助益如何?

How has the employed person, during his or her term of employment, benefited your unit or the relevant domestic academic field? 受聘者蔡世傑博士專注於科學研究,也樂於指導碩博士生,與他們分享研究經驗與實驗成果。尤其對新進的學生助益更大, 特別是在實驗動物的照護方面的專業,一方面減少摸索的時間,一方面可確保實驗鼠狀況穩定,確保後續實驗數據的可靠度。 (5)具體工作績效或研究或教學或科技研發與管理成果:

Please describe the specific work performance, or the results of research, teaching, or R&D and management work:

受聘者蔡博士這幾年主要工作在於基因編輯(gene editing)的技術服務.近三年為國內研發團隊產製 超過 40 例基因改造鼠及疾病模式鼠。其相關論文與研發成果,預期於近年內陸續完成。

(6)是否續聘受聘人? Will you continue hiring the employed person? □續聘Yes ■不續聘No ※ 此報告表篇幅以三~四頁為原則。This report form should be limited to 3-4 pages in principle. ※ 此表格可上延攬優秀人才成果報告繳交說明網頁下載。

數據

Figure 1. Genotyping of COL1A1  and  COL1A2  mutant rat.  The designed primer pair  recognize COL1A1 mutant DNA only with a 1723 bp amplicon

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