Inflammatory Myofibroblastic Tumor: A Rapidly Growing Soft Tissue Mass in the Posterior Mandible

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S I N E Q U A N O N C L I N I C O P A T H O L O G I C C O R R E L A T

Inflammatory Myofibroblastic Tumor: A Rapidly Growing Soft Tissue Mass in the Posterior Mandible

Parul Sah Aditi Amit Byatnal Lakshmi Rao Venkadasalapathy Narayanaswamy

Raghu Radhakrishnan

Received: 10 May 2013 / Accepted: 27 June 2013 / Published online: 11 July 2013 Ó Springer Science+Business Media New York 2013

Abstract The term inflammatory myofibroblastic tumor (IMT) encompasses a diverse group of spindle cell entities that traverses a clinical and histologic spectrum, extending from reactive to benign neoplastic to highly aggressive with malignant inclinations. Head and neck IMTs are rarely seen and comprise less than 5 % of tumors. Here we report a case of a 30 year old male who presented with a rapidly enlarging and extremely painful growth in the right pos- terior mandible, post extraction. Histopathological exami- nation revealed a highly cellular connective tissue stroma comprised of spindle shaped cells arranged in fascicles, admixed with inflammatory cells, predominantly plasma cells. Apart from routine hematological investigations, serum protein electrophoresis was also performed. The final diagnosis was confirmed by a panel of immunomar- kers consisting of MPO, CD34, CD20, CD3, CD23, CD138, SMA and ALK. To the best of our knowledge, this is the third case of oral IMT arising from an extraction socket.

Keywords Inflammatory myofibroblastic tumour Inflammatory pseudotumor Gingiva  Plasma cells  Myofibroblasts Immunohistochemistry

Case Presentation

A 30-year old male presented with a painful, rapidly enlarging growth in the right posterior mandible of 1 month duration. The patient had undergone extraction of the right mandibular third molar about 4 months back, with persistence of postoperative pain in that region. The med- ical history was unremarkable. Clinical examination revealed a 7 9 5 cm reddish pink mass with an irregular surface in the right mandible. The mass extended from the extraction socket of the third molar to the canine and involved both buccal and lingual surfaces, almost covering the occlusal aspects of the teeth (Fig.1a). The swelling was soft in consistency and elicited tenderness on palpation.

Routine hematological investigations revealed mild leu- kocytosis (15,400 cells/mm3) and an elevated erythrocyte sedimentation rate (107 mm). Fine needle aspiration cytology and histopathological examination following an incisional biopsy were inconclusive. The lesion continued to display rapid growth with involvement and progressive mobility of all the posterior teeth. Computed tomography of the mandible in the transverse plane revealed a hypo- dense area involving the premolar and molar region, associated with a breach in the continuity of the lingual cortical plate (Fig.1b). 3-dimensional CT reconstruction confirmed this to be an osteolytic lesion giving the impression of an aggressive neoplasm, which necessitated repeating the incisional biopsy at two representative areas of the lesion.

Parul Sah and Aditi Amit Byatnal have contributed equally to the manuscript.

P. Sah A. A. Byatnal  R. Radhakrishnan (&) Department of Oral and Maxillofacial Pathology, Manipal College of Dental Sciences,

Manipal University, Manipal 576104, Karnataka, India e-mail: raghu.radhakrishnan@gmail.com

L. Rao

Department of General Pathology, Kasturba Medical College, Manipal University, Manipal 576104, Karnataka, India

V. Narayanaswamy

Craniofacial and Dental Hospital, K. K. Nagar, Madurai 625020, Tamil Nadu, India

DOI 10.1007/s12105-013-0474-8

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Histopathological examination revealed a highly cellular connective tissue stroma with the overlying epithelium showing surface erosion. The cellular proliferation com- prised of spindle shaped cells arranged in a fascicular growth pattern, admixed with a dense inflammatory cell infiltrate consisting of plasma cells, eosinophils, neutro- phils and lymphocytes. The vascular component, present as slit-like spaces, was obscured by background cellularity.

(Fig.1c, d)

Diagnosis

The differential diagnoses of a rapidly growing soft tissue lesion causing osteolysis comprise reactive lesions, benign neoplasms, connective tissue malignancies and hemato- logic pathologies.

The proliferating endothelial cells, demonstrated by CD34 staining, in an abundant inflammatory background suggested a granulation tissue associated with healing of extraction wound [1]. The plasma cell gingivitis was con- templated because of the erythematous, diffuse gingival enlargement, with predominant plasma cell component and psoriasiform epithelium [1]. The aggressiveness of the lesion and destructive growth noted in the present case, along with fascicular pattern of spindle cells in a mixed inflammatory infiltrate ruled out these reactive lesions.

Abundance of plasma cells in an osteolytic lesion con- firmed by CD138 positivity (Fig.2a), indicated a possible diagnosis of plasmacytoma [2]. However, the admixture of polymorphs and spindle cells, absence of monoclonal gammopathy and expression of both the kappa and lambda light chains confirmed with serum protein electrophoresis, conclusively ruled out plasmacytoma.

Myeloid sarcoma, the extramedullary manifestation of acute myeloid leukemia, was considered because of the conspicuous granulocyte population in our case. Despite the presence of a mixed inflammatory infiltrate dominated by plasma cells, primitive myeloid cells (blasts) were not evident in our case [4]. While the mature myeloid cells of neutrophilic and eosinophilic lineage stained positively for myeloperoxidase (MPO) (Fig.2b), other inflammatory cells failed to express MPO, thus eliminating the diagnosis of myeloid sarcoma.

The predominant lymphocyte population and rapidly growing lesion enforced us to rule out a T or B cell lym- phoma. The focal positivity of CD3 (Fig.2c) and CD20 (Fig.2d) along with normal hematologic laboratory values precluded the diagnosis of lymphoma.

Since the background component was spindle cell pre- dominant, staining with SMA was performed. Strong posi- tivity for SMA noted in our case suggested a myofibroblastic differentiation (Fig.2e). Nodular fasciitis presents as a rapid

growth with high cellularity and short linear curved fascicles of spindle cells. Follicular dendritic tumor presents as a fascicular arrangement of spindle cells along with an inflammatory infiltrate and shows strong positivity for CD21 [5]. The presence of longer fascicles of spindle cells and a plasma cell predominant inflammatory infiltrate in our case ruled out both these entities. The lack of necrosis and absence of staining for CD23 used as a substitute for CD21, further confirmed the exclusion of nodular fasciitis and follicular dendritic tumor, respectively.

Inflammatory myofibroblastic tumor (IMT) or plasma cell granuloma is a distinctive lesion composed of myo- fibroblastic spindle cells accompanied by an inflammatory infiltrate rich in plasma cells, lymphocytes, and eosinophils with occasional presence of neutrophils. It occurs primarily in soft tissue of children and young adults and does not demonstrate any hematological aberrations or systemic symptoms. An aggressive and rapid growth pattern is often noted [5,6]. Our case presented with all the above features.

After considering the above differential diagnosis, the lesion was eventually signed out as an inflammatory myofibroblastic tumor. Approximately 8–60 % of IMTs exhibit chromosomal translocation of the anaplastic lym- phoma kinase (ALK) gene and immunoreactivity for the ALK protein [7]. However, the present case was negative for ALK immunoexpression.

The patient was given a course of steroids following which the lesion resolved. The patient has been under regular follow-up since 7 months and there has been no evidence of recurrence.

Discussion

The term inflammatory myofibroblastic tumor (IMT) encompasses a diverse group of spindle cell lesions that traverses a clinical and histologic spectrum, spanning from reactive non neoplastic to highly aggressive lesions with malignant inclinations [7]. First reported by Brunn et al. in the lung [8], this lesion was reported under a varied nomenclature until the WHO termed it as inflammatory myofibroblastic tumor, a unique lesion comprising myo- fibroblastic spindle cells and an inflammatory infiltrate of plasma cells, lymphocytes, and eosinophils [3].

IMT is predominantly a tumor of the viscera, [9] and head and neck sites comprise less than 5 % of tumors, with the buccal mucosa, posterior mandible, tongue and gingiva being frequent oral locations [7, 10]. To the best of our knowledge, this is the third case of IMT arising from the extraction socket to be documented [6]. Occurrence in almost all age groups has been reported [9], but IMT shows a distinct predilection for children and young adults [3].

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Oral lesions develop over a short duration of time and often exhibit a disturbingly rapid growth rate [7].

The WHO delineates three primary histological patterns of IMT—a ‘‘myxoid/vascular pattern’’, a ‘‘compact spindle cell pattern’’ with fascicular architecture and a distinctive infiltrate of plasma cells, and a ‘‘hypocellular pattern’’ with heavy collagenisation, bearing resemblance to fibrous scar tissue [3, 9, 11]. The present case exhibited a compact spindle cell pattern.

The etiopathogenesis of IMT remains unresolved. These lesions were traditionally considered a benign reactive process [12], possibly as an exaggerated immune response to Epstein–Barr virus, human herpes virus-8, trauma, sur- gery or a foreign body [3,6]. At least a fraction of IMTs appear to be neoplastic in nature, based on reports of recurrence [11], regional metastases [3], extensive local invasion leading to death in one case, and malignant transformation [11]. This concept has been further sup- ported by evidence of aberrations in the anaplastic lym- phoma tyrosine kinase (ALK) receptor locus on chromosome 2p23, reported in up to 50 % of IMTs [9].

Chromosomal rearrangement of the ALK gene appears to promote aberrant phosporylation and may induce tumori- genesis [7]. ALK expression was negative in our case.

The controversy over the neoplastic nature of IMT hovers over its treatment, with surgical excision [5], curettage [13], corticosteroid therapy [3], radical surgery [13] and radiotherapy [6], as possible options. Although complete surgical resection is widely reported as the treatment of choice [3,6,9], instances of lesional resolu- tion achieved by conservative modalities [5] as in our case, have reinforced the ambiguity over the nature of IMT.

Pankaj et al. [13] have noted no instance of recurrence reported in the head and neck or oral region so far. They hypothesized that IMT occurring in head and neck sites behave distinctly from extra-oral IMT, thus advocating a different treatment protocol. Prudent therapeutic interven- tion in any case is mandatory, considering the rapid growth the lesion exhibits, and postoperative follow up for at least 10 years is mandatory to exclude recurrence and metastasis [7].

Multiple entities resemble IMT and must be excluded via a complete pathologic workup, utilizing immunohis- tochemistry and hematology. Since the clinical behavior of IMT mimics malignancy, a thorough investigation is compulsory to avoid needlessly radical treatment modali- ties. A potentially aggressive course of IMT mandates regular follow up.

Fig. 1 aClinical photograph showing a soft tissue mass arising from extraction socket of right mandibular third molar. b Axial CT highlighting osteolytic areas with destruction of lingual cortical plate.

cHighly cellular connective tissue stroma with surface erosions and

psoriasiform epithelium (Haematoxylin and eosin, 9100). d Intense proliferation of plasma cells with scattered lymphocytes, neutrophils and eosinophils. (Haematoxylin and eosin, 9400)

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