! "Parathyroid hormone restores bone mass for osteopenia in the
spontaneously hypertensive rat #$NSC87-2314-B-039-017 %&'(86/08/01~87/07/31 )*+,-. %&/0123456789:58;<= E-mail[email protected] > ? 19 @ A
(spontaneously hypertensive rat, SHR)BCD /EFGHI 4 JBK-LMNOPQR HSTUHVWX 0Y4Y8 Z 16 [\/100g + ] (human parathyroid hormone, hPTH1-34)B^_ 14 -BL`a XbcdefghiB21 @jkl hPTH SHRBmnop q
Xrstu Wistar Kyoto (WKY rat) vwxByg0z{rIq|f}~q Bjbf hPTH JBmnop q X1 Bs~1 9 def H1 ¡ B¢ 16 [\ hPTH J£xB ¤J¥j¦§ ¨©fªz«B hPTH¬ ®¯°B ±²j¦§B³´X µhf¶MNOP hPTH · X /¸B¹º»¼½f¾J¿e À7Á ÂÃiBhPTH J¿ Ä Å®ÆDZ²B)ÈÉÊc Ë Ì ÍÎÏf Abstract
Daily subcutaneous injection of graded doses of 0, 4, 8, or 16Ðg / 100g hPTH 1-34 of 19-week-old male spontaneously hyperten- sive rat (SHR) of five rats in each group for 14 days were done. The 21-week-old SHR exhibited a significant lower metaphyseal trabecular bone volume osteopenia accompanied by poor trabecular architecture than in Wistar-Kyoto (WKY) rats were observed in the study. The preservation of trabecular bone volume in the PTH-treated rats was associated with an increase in number of osteoblasts, bone surface extent of osteoblasts, double labelled bone surface, mineral apposition rate and trabecular thickness. Trabecular number remained comparable to control. Measurements of resorption such as percent eroded surface and numbers of osteoclasts also remained comparable to control values. These dual effects increase the bone formation rate and in the absence of significant resorption, result in a net increase in bone mass. The above findings of histomorphometric data support the hypothesis that the PTH increase bone mass by specific effects on osteoblast activation and function.
Key word: Parathyroid hormone, osteo-porosis, spontaneous hypertensive rat, osteopenia, bone cell.
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q XÉj SHR JrB ¦§ deByD WXB÷â TUf (V)YmnopWX:Á bc IY WXWKY : SHR J§,j SHR Jf IIYWXYZWKY : SHRJ¦§ j SHR Jf IIIY WKY : SHR J¦§âj SHR Jf IVY[\W¯°WKY : SHRJ¦§j SHR Jf (])Ymnop:Á bc IY23WWKY : SHRJ¦§â j SHR Jf IIY 23W WKY ¦§â SHRfL 4 Z 8 [ \ hPTH SHR JB¦§ j SHR Jf 16 [ \ JÉj SHR J rB¥)¦§¨©f IIIY 1u SHR ¦§ WKY fj SHR JB{ hPTH : SHR Jf IVY ^_WKY ¦§ SHRfL 4 Z 8 [ \ hPTH SHR JB¦§ j SHR Jf 16 \ SHR JÉj SHR JrB¥)C7z ¨©f VY WKY ¦§ SHRfL 4 Z 8 [ \ hPTH SHR J¦§ j SHR Jf 16 [ \ SHR JÉj SHR JrB¥)C7z¨©f $`%YmnopaÀÁ bc IY HWKY ¦§ SHRfL hPTH : SHR J ¦§j SHR JBy DWX÷ TUf IIY WKY ¦ § SHRfL hPTH : SHR J¦§j SHR JfL 16 [\ hPTH J Ibâf IIIY:JcjB C7z ¨©f IVY WKY ¦§x SHRfL hPTH : SHRJx¤JByDWX B÷ TUf (d)Ymnop ÀÁ bc q e fg¯°Y hijkBhijkH1 ®¯°B WKY ¦§ SHRf÷L hPTH : SHR JB¦§j SHR JB yDWXB÷ if VY%& ? SHR 21 @ ¼B=?@1>? @Arst WKY ¤Jxf}g hÉ Bindels l 1987 Úmn gh os(7)fTU hPTH B>? @Af}p·ÎÉq>? @ Zrs>?@ ' ±²(8)Ïf E>FGAB·t±
ÍÎ uj(marker)fAD, Ê ÷By ÉHc®Ï(9)f hPTH v SHR JB¼E>FG¦§ BI Ê ðw>xyzf ¼L M NFOAÆrÍ ÎujBr SHR É WKY {JD )¦§bcf}ghÉ McCarron l+ ios(10)fL å&| }~FB r M±²Buj·ñ r ÍÎ(11)fÞ}B hPTH B¼L MA÷NF D)SbcB·ÎåÍΩZå &| ÆDZ²³f SHR mnoGpq XB¦§ iB÷ hPTH v 14 - BXܦ§ ± ²fghÉ ®X (12) ßqàH(13) l hPTH B¬q X ghå>³ f SHR ® Î (bone-forming activity) WKY ¤JxB}ghÉ, -.l(14) 26 @ SHR mJ¿ o if hPTH v 14 - SHRJB®Îcjkl hPTH v SHR JvwByDWX TUf}p¦ hPTH ¬Ë Ì9(osteo-progenitor cells) Hc±²(15)f hPTH v B o ÏÁBjkl hPTH v SHR J1 SHR+16Ðg hPTH JÉj SHR J)¦§¨©f¾}«B »sWX hPTH ¬Ä» s ±²ByrxWX(SHR+4 or 8Ðg ÍÎB÷rWX (SHR+16Ðg hPTH )~B¬k ÍÎf hPTH v SHR JB aÀÁ bcghÉGunnessl(16) ² hPTH v ghofhPTH ¬´ g0 rB*åLfg QRq XBs~{¬´ W (biomechanical competence)f hPTH v SHR JBe fg¯°¦§ jkl hPTH SHR Jf}ghÉ B hPTH B·Lie fg¯° i(12)of}p ¦ hPTH ® ±²f '()*$+ , &~B× vB:x ÁuX±BòCH< ¡ BL³gh ¢£f !®h¤B 7¥Á¦§(B·ÉL Y|H¨ ©J¿loÏ 57ª« zf '-./ 1.ÒÓ4ÔÕôõÓ479 Ú 80 Ú+ Ö¬B&®8 ¯ :°±¬+ ²³v#´B1992f
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