[PDF] Top 20 Gene cloning and biochemical characterization of chitinase CH from Bacillus cereus 28-9
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Gene cloning and biochemical characterization of chitinase CH from Bacillus cereus 28-9
... study of Pleban et ...B. cereus strain ...activity of strain ...activity of mild ...supernatant of B. cereus 28-9, we presume that not only ChiCH but also other ... See full document
9
Identification of an antifungal chitinase from a potential biocontrol agent, Bacillus cereus 28-9
... 2004 Bacillus cereus 28-9 is a chitinolytic bacterium isolated from lily plant in ...blight of lily as demonstrated by a detached leaf assay and dual culture ...(ChiCW ... See full document
7
Molecular Cloning and Characterization of Fengycin Synthetase Gene fenB from Bacillus subtilis
... 2 AND SHIH-TUNG LIU 2 * Graduate Institute of Botany, National Taiwan University, Taipei, 106, 1 Graduate Institute of Botany, National Chung-Hsing University, Taichung, 402, 3 and Molecular ... See full document
4
High-level expression and characterization of two chitinases, ChiCH and ChiCW, of Bacillus cereus 28-9 in Escherichia coli
... ChiCH and ChiCW were successfully highly expressed in ...proteins, and recombi- nant native ChiCH and ChiCW could be purified after cleavage with PreScission protease to remove GST ...for ... See full document
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Purification, crystallization and preliminary X-ray crystallographic analysis of chitinase from Bacillus cereus NCTU2
... conserved and that Glu145 and Tyr227 are potential residues that mediate the catalytic function of ...mechanism of ChiNCTU2, we have cloned the cDNA encoding chitinase and ... See full document
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Molecular Cloning, Characterization, and Expression of a Chitinase from the Entomopathogenic Fungus Paecilomyces javanicus
... strong chitinase activity was detected in ...cloned from mycelia by using both degenerate primer/reverse transcription polymerase chain reaction (RT-PCR) amplification and 5¢-/3¢-RACE ...cDNA ... See full document
6
Cloning, Expression, and Characterization of Two β-Glucosidases from Isoflavone Glycoside-Hydrolyzing Bacillus subtilis natto
... Overexpression and Purification of the Two Recombi- nant ...the biochemical proper- ties of BglH and YckE, we expressed the six-histidine N terminal-tagged proteins in ...vector ... See full document
7
Identification of a gene encoding Lon protease from Brevibacillus thermoruber WR-249 and biochemical characterization of its thermostable recombinant enzyme
... extraction of genomic DNA, PCR-mediated amplification, and sequencing of the purified PCR product, the 16S rDNA sequence was compared with the previously determined Bacillus sequences available ... See full document
11
Cloning, characterization and phylogenetic relationships of stxI, a endoxylanase-encoding gene from Streptomyces thermonitrificans NTU-88
... characteristics of STXI STXI remained active over a very broad pH range of 5–9 and xylanase was active between 30 and 60 °C with an opti- mum at around 50 ...bility from 10 to 50 ... See full document
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Vacuolar Invertases in Sweet Potato: Molecular Cloning, Characterization, and Analysis of Gene Expression
... Institute of Microbiology and Biochemistry and Department of Biochemical Science and Technology, National Taiwan University, Taipei 106, Taiwan Two cDNAs (Ibβfruct2 and ... See full document
7
Production of Chitinase and N-Acetylchitooligosaccharides from Bacillus sp. DYU-Too 20 陳岳澤、吳淑姿
... Assessment of Capability of Fetal and Adult Neural Stem Cells to Differentiate into Endothelial ...Journal of Kurdistan University. 13: 1-12. 16.Feng, J., Zhao, L., and Yu, ...effect ... See full document
3
Molecular cloning and functional characterization of hemolysin gene from Vibrio furnissii
... effect of metabolites of flavonoid is ...MRP4 and MRP5, and that some of them, i.e. quercetin and silymarin, may well prove to be substrates for ...function and ATPase ... See full document
16
Cloning and characterization of monacolin K biosynthetic gene cluster from Monascus pilosus
... transcription of the predicted monacolin K biosynthetic genes, Northern hybridizations were performed with DIG-labeled ...Transcripts of the putative monacolin K biosynthetic genes could be detected on the ... See full document
8
Cloning and characterization of the carp prolactin gene
... The transcription start site was determined by primer extension using carp pituitary mRNA ~s template.. The primar extension was carried out w~th the mouse Molo- ney leukem[r] ... See full document
4
Crystal Structures of Bacillus cereus NCTU2 Chitinase Complexes with Chitooligomers Reveal Novel Substrate Binding for Catalysis A CHITINASE WITHOUT CHITIN BINDING AND INSERTION DOMAINS
... 2010, and in revised form, July 27, 2010 Published, JBC Papers in Press, August 4, 2010, DOI ..., and Chun-Jung Chen ‡储 ** 3 From the ‡ Life Science Group, Scientific Research Division, National ... See full document
14
Biochemical characterization and cloning of transglutaminases responsible for hemolymph clotting in Penaeus monodon and Marsupenaeus japonicus
... hemocytes of Penaeus monodon (abbreviated as TGH) was ...plasma and incorporated fluorescent dansylcadaverine into succinyl casein upon activation by CaCl 2 in ...mass-analyses of tryptic peptides ... See full document
12
Biochemical Properties and cDNa Cloning of Two New Lectins from the Plasma of Tachypleus tridentatus
... TPL-1 and TPL-2 were isolated from ...determined, and their biochemical properties inves- ...consist of small molecular weight subunit of protein with mass of 15–25 kDa, ... See full document
9
Cloning and characterization of the mouse histone deacetylase-2 gene.
... The mouse HDAC2 gene spans over 36 kilobase pairs and is composed of 14 exons (ranging from 58 to 362 nucleotides in length) and 13 introns (ranging from 75 base pairs to 19 kilobase pa[r] ... See full document
1
Molecular Cloning, Characterization, and Transgenic Expression of CCFMADS: a MADS-Box Gene from Calocedrus formosana
... MADS-box gene, CCFMADS, was cloned from Calocedrus ...analyses and similarities of specific C-terminal motifs, this gene was cat- egorized into C-function lineages belonging to the ... See full document
16
Purification, crystallization and preliminary X-ray crystallographic analysis of glycosyltransferase-1 from Bacillus cereus
... Jean and supporting staff for technical assistance with and discussion of the use of the synchrotron-radiation X-ray facility for data collection at BL13B1 of the NSRRC, Taiwan, Masato ... See full document
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