ӧаଳײნೀ 21 Ϻϐ 35S::OsADF2 Ϸ 35S::OsADF11 ޑᙯਲ਼ᢀჸ ځϸᔈǴӧೀ 7 Ϻࡕჴᡍڋಔ (Negative control) ϐ N3 (atnced3) ယТς рᝄख़ǵׇॹҷຝǴӕਔഁғࠠ Col-0 ΨрယТያᕭǵׇॹҷǵ
ߙનಕᑈϐຝǴԶ 35S::OsADF2 ᙯਲ਼Οঁࠔس (OsADF2-3ǵOsADF2-4ǵ OsADF2-6) ύᆶჹྣಔ࣬КᗨԖڙډӢଳײԶԋϐғߏ෧ᄌǴՠࢂϝ߄
ᆢ҅தғߏǴځύᗨ OsADF2-3 ࠔسϐਲ਼ယТԖрߙનಕᑈຝǴՠ
ނϐယТǵׇࣣᆢ҅த߄ǴӢԜёаፕНዿ OsADF2 ዴჴᔈᆶ
ނ य़ ᖏ ଳ ײ ნ ϐ ϸ ᔈ Ԗ ᜢ ǹ Զ 35S::OsADF11 ޑ ᙯ ਲ਼ Ο ঁ ࠔ س (OsADF11-1ǵOsADF11-2ǵOsADF11-3) ύࣣ߄ᆢ҅தғߏǴЪғߏࠠᄊ ᆶჹྣಔ࣬Кคܴᡉৡ౦ǴယТϝև଼நރᄊǶௗុаଳײೀಃ 12 Ϻࡕёᢀჸډ N3 ςֹӄǴഁғࠠ Col-0 ߾ࢂယТԔϐำࡋΜϩᝄ ख़Ǵ 35S::OsADF2 ᙯਲ਼ΟঁࠔسϐယТ߾ࢂ໒ۈрԔያᄙϐຝǴЪ
ׇрॹҷޑຝǴԶ 35S::OsADF11 ޑᙯਲ਼Οঁࠔسନ OsADF11-1 ࠔس ϐယТԔϐำࡋၨεѦǴځдٿঁࠔسϐယТр٤ያᖩԔǶ
ௗՉൺНࡠൺࡕᢀჸਲ਼ϐࡠൺϸᔈǴൺНಃϺ 35S::OsADF2 ᙯਲ਼ ΟঁࠔسаϷ 35S::OsADF11 ޑᙯਲ਼ΟঁࠔسࣣҥջԖܴᡉӣൺϐຝрǴ
46
Զа 35S::OsADF11 ޑᙯਲ਼ϐӣൺਏ݀ၨ٫Ǵځύբࣁ҅ӛڋಔϐ OE2 ( 35S::LfNCED3) ᙯਲ਼ΨԖ٤ӣൺϐ߄Ǵॄӛڋಔϐ N3 ߾ࢂคӣൺ (Eun et al., 1997) ǶԶӧԋਲ਼ϐଳײნೀϩύᡉҢǴ35S::OsADF2 ᙯ
ਲ਼ӧаଳײೀ 12 ϺࡕယТϐำࡋၨεǴฅԶ OsADF11 ᙯਲ਼Ԗ٤
ယТያᕭຝǴЪҗൺНࡕϐࡠൺ߄ύёᢀჸډᗨฅ OsADF2 Ϸ OsADF11 ᙯਲ਼ࣣԖܴᡉၨഁғࠠ Col-0 Ϸჴᡍჹྣಔ OE2 ϐӣൺૈΚଯǴསҢН
ૈёаճҔ Actin binding protein ᆶ GFP ϐᑼӝೈқ (ABP-GFP) ᙯܭߓ
47 2007)ǶҁጇፕЎ٬Ҕ PEG ᙯמೌՉНዿϐ OsADF1-GFPǵOsADF2-GFPǵ OsADF3-GFPǵOsADF4 –GFP ǵOsADF5-GFP ǵOsADF6-GFP ǵOsADF7-GFP ǵ OsADF11-GFP ᑼ ӝ ೈ қ ϐ ԛ ಒ झ ۓ Տ ϩ Ǵ ่ ݀ ว Н ዿ OsADF1-GFP ǵ OsADF2-GFPǵOsADF4-GFPǵOsADF5-GFP Ϸ OsADF6-GFP ᑼӝೈқ߄Տܭယ ᆘᡏǴԶ OsADF2-GFPǵOsADF3-GFP Ϸ OsADF11-GFP ᑼӝೈқ߄Տܭಒझਡ Ϸಒझ፦Ǵځύ OsADF2-GFP ӕਔӸӧܭಒझਡ፦аϷယᆘᡏύ߄ǴаϐН ዿচғ፦ᡏԛಒझۓՏჴᡍࣣख़ፄΟԛǴ٠ЪࣣԖа CYH8-Ub::GFP բࣁჹྣಔ ዴۓᙯࢂցԋфǴ٠Ъीᆉᙯਏ (ᙯԋф > 50ǴջຎഁΠԿϿԖъ AtADF2ǵAtADF3ǵAtADF4ǴԶ F ဂ߾ࢂѝ֖ԖНዿ OsADF7ǵOsADF8 Ϸ OsADF10Ǵ
48
ᡉҢёૈڀԖځϯϐ߄ՏǴЪҗΓࣴزύࣣԖࡰр ADF ёૈୖᆶ٤
ނಒझϣϐૻ৲ሀǴՠࢂނϐ ADFs ೈқࢂցᆶނϐ ADF/Cofilins ኬёբ ࣁ ௗ ڙ ڈ ᐟ ϸ ᔈ ϐ ፓ ޣ Ǵ Ҟ ς Ԗ Ᏽ ᡉ Ң PIP2 (phospholipid phosphatidylinositol-4,5-bisphosphate) аϷ protein kinase C ୖᆶ ADF ᆶಒझମࢎϐ ໔ᄊѳᑽǴฅԶځ၁ಒϐᐒڋϝฅ҂ޕ (Nick, 2013) ǴԶယᆘᡏϐၮ౽аϷф
ૈ߄ΨڙډಒझମࢎϐፓǴӢԜፕԜ٤Нዿ ADF ߄ܭယᆘᡏёૈᆶယᆘ ᡏϐ٤࣬ᜢϐғ৩࣬ᜢǴฅځфૈϷୖᆶ৩ϝۘࡑࡕុჴᡍӆǶ
Ѥ
Ѥǵ ่ᇟ
ނϐಒझମࢎᆶځғߏวػǵғࢲаϷߚғނნऐڙ܄৲৲࣬ᜢǴ ځᛙۓ܄ӧނय़ᖏߚғނნԶقࢂߚதख़ाޑǶҁጇࣴزЬाݙܭНዿϐ OsADF2 Ϸ OsADF11 ϐфૈ܄ࣴزǴᙖҗ౦Տεໆ߄ OsADF2 Ϸ OsADF11 ୷Ӣ ܭߓդਲ਼ύࣴزԜΒ୷ӢϐғфૈǶҗаᅿᅿ่݀ᡉҢεໆ߄
OsADF2 аϷ OsADF11 ϐߓդᙯਲ਼ၨഁғࠠёૈڀԖଯᡶნаϷଳײ
ნऐڙ܄ǴฅԶځύ܌ୖᆶϐғᐒڋǴϝӆа୷Ӣ߄ϩǴаϷځдғ
ࡰፓϩǴаࣴزНዿ OsADF2 аϷ OsADF11 ӵՖቹៜނӧଯᡶϷଳײ
ნΠϐნऐڙ܄Ƕ
49
ୖ
ୖԵЎ
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