Pa tch ou li
0.01%
0.05%
0.1%
In h ib it o ry e ff e c t, %
Figure 6. Inhibitory effect of E. coli growth by 0.01%, 0.05% and 0.1% of seven essential oils after 24 hours of incubation.
Conclusions
We have demonstrated that GC−MS with database NIST 08 is an ideal tool for essential oils routine analysis. Though seven essential oils from Taiwan consist of complex compositions, some of them have high percentage of few compounds. Those essential oils are subject to specific applications. For example, patchouli and clove basil have high content of carvacrol and eugenol; respectively. Both of the compounds are very effective antibacterial reagents, and not surprised that patchouli and clove basil exhibit excellent inhibition to E. coli. The compositions of essential oils are highly dependent on the growing conditions of the plants. We found tea tree essential oils from Taiwan has high percentage of 1,8-cineole and makes its unique flavor.
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Supporting Information
0 20 40 60 80
0 500 1000
0 20 40 60 80 100
0 500 1000
Normalized Abundance
100 200 Time, min
(b) (a)
Time, min
Normalized Abundance
100 200
Temp, o CTemp, o C
Figure S1. GC−MS chromatogram for lemon verbena essential oil. (a) linear gradient.
(b) step gradient.
0 20 40 60 80
Figure S2. GC−MS chromatogram for sweet marjoram essential oil. (a) linear gradient. (b) step gradient.
0 20 40 60 80
Figure S3. GC−MS chromatogram for clove basil essential oil. (a) linear gradient. (b) step gradient.
0 20 40 60 80
Figure S4. GC−MS chromatogram for patchouli essential oil. (a) linear gradient. (b) step gradient.
Figure S5. GC−MS chromatogram for rosemary essential oil. (a) linear gradient. (b) step gradient.
0 100 200 300 400 500 0.0
2.0x106 4.0x106 6.0x106
Abu n d a n c e
ppm geraniol
Y = -234687 + 14189*X R 2 = 0.97546
OH
Figure S6. The regression relationship between the concentration of geraniol and its integrated area of abundance in the GC−MS chromatogram.
0 100 200 300 400 500
0.0 4.0x106 8.0x106 1.2x107