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表一、中藥酒精萃取物冷凍乾燥後所得的重量及實驗刺激的濃度 酒精萃取物
乾燥前體 積(ml)
乾燥後
重量(g) 濃縮液(ul) 刺激量(mg) 實驗刺激的濃度 (mg/ml)
黃柏 5 0.0598 25 0.299 0.0299
黃連 5 0.0711 25 0.3555 0.03555
鴉膽子 5 0.1601 25 0.8005 0.08005
苦參 5 0.0082 25 0.041 0.0041
秦皮 5 0.0342 25 0.171 0.0171
大黃 5 0.0470 25 0.235 0.0235
巴豆 5 0.0174 25 0.087 0.0087
牽牛子 5 0.0638 25 0.319 0.0319
火麻仁 5 0.0256 25 0.128 0.0128
表二、中藥水萃取物冷凍乾燥後所得的重量及實驗刺激的濃度 水萃取物
乾燥前體 積(ml)
乾燥後
重量(g) 濃縮液(ul) 刺激量(mg) 實驗刺激的濃度 (mg/ml)
黃柏 5 0.0311 25 0.1555 0.01555
黃連 5 0.0269 25 0.1345 0.01345
鴉膽子 5 0.0202 25 0.0101 0.00101
苦參 5 0.0290 25 0.145 0.0145
秦皮 5 0.0334 25 0.167 0.0167
大黃 5 0.0184 25 0.092 0.0092
巴豆 5 0.0214 25 0.107 0.0107
牽牛子 5 0.0646 25 0.323 0.0323
火麻仁 5 0.0040 25 0.02 0.002
表三、離子成分分析 離子成分
Mg(mM) K(mM) Na(mM) Ca(mM)
黃柏 0.49 2.09 0.21 0.0525
黃連 0.84 2.82 0.34 0.085
鴉膽子 7.57 0.74 undectable undectable
苦參 4.23 1.91 0.61 0.1525
秦皮 5.42 1.66 0.04 0.01
大黃 5.57 0.15 undectable undectable
巴豆 2.35 1.87 0.09 0.0225
牽牛子 1.93 1.76 1.26 0.315
火麻仁 2.6 5.7 2.79 0.6975
表四、不同藥材酒精抽出物對於 Forskolin 刺激大白鼠腸道上皮組織 間離子電流的影響，數值單位為μA，括弧內為標準差。
藥材 樣本數 起始值 加 Forskolin 加抽出物 電流變化值
對照組 3 57.3 105.7 105.0 -0.7(1.5)
黃連 6 58.8 109.2 95.7 -13.5(8.4)
黃柏 6 47.3 103.2 96.2 -7.0(2.4)
鴉膽子 6 50.0 94.0 86.0 -8.0(3.0)
苦參 6 52.3 79.3 73.7 -5.6(1.7)
秦皮 6 35.3 88.5 80.0 -8.5(2.0)
大黃 6 45.2 95.5 109.8 +14.3(3.1)
巴豆 6 42.0 98.7 113.3 +14.6(3.0)
牽牛子 6 40.8 95.3 113.7 +18.4(4.1)
火麻仁 6 47.0 99.7 112.8 +13.1(2.9)
表五、不同藥材水抽出物對於 Forskolin 刺激大白鼠腸道上皮組織間 離子電流的影響，數值單位為μA，括弧內為標準差。
藥材 樣本數 起始值 加 Forskolin 加抽出物 電流變化值
對照組 3 57.3 105.7 105.0 -0.7(1.5)
黃連 3 62.4 109.2 107.2 -2(3.46)
黃柏 3 45.2 110.6 107.2 -3.4(1.15)
鴉膽子 3 53.8 121.4 119.0 -2.4(1.15)
苦參 3 56.8 110.3 105.9 -4.4(1.53)
秦皮 3 43.3 91.2 90.2 -1(1.0)
大黃 3 43.9 98.7 100.4 +1.7(0.57)
巴豆 3 54.3 113.6 120.3 +6.7(2.08)
牽牛子 3 48.4 110.4 113.0 +2.6(1.52)
火麻仁 3 54.1 109.5 112.2 +1.7(1.15)
圖一、forskolin 對於大白鼠腸道短路電流的影響。大白鼠腸道上皮在 架上 Ussing chamber 後，箝制上皮兩端電壓同時，可測得上皮間一短 路電流，此電流約在 40µA 左右，為上皮細胞進行正常離子主動運輸 所造成，在漿膜端加入 5µM forskolin，引發細胞內 cAMP 增加並導 致氯離子分泌增加，而使短路電流驟升至 70µA 上下，此上升之短路 電流通常可持續一兩個小時。
圖二、黃柏酒精抽出物對於接受 forskolin 刺激大白腸道上皮短路電流 的影響。接受 forskolin 刺激大白鼠腸道上皮，在加入黃柏酒精抽出物 後，短路電流明顯下降。
圖三、秦皮酒精抽出物對於接受 forskolin 刺激大白腸道上皮短路電流 的影響。接受 forskolin 刺激大白鼠腸道上皮，在加入秦皮酒精抽出物 後，短路電流明顯下降。
圖四、巴豆酒精抽出物對於接受 forskolin 刺激大白腸道上皮短路電流 的影響。接受 forskolin 刺激大白鼠腸道上皮，在加入巴豆酒精抽出物 後，短路電流明顯上升。
圖五、大黃酒精抽出物對於接受 forskolin 刺激大白腸道上皮短路電流 的影響。接受 forskolin 刺激大白鼠腸道上皮，在加入大黃酒精抽出物 後，短路電流明顯上升。
圖六、不同藥材酒精抽出物對於 forskolin 刺激大白鼠腸道上皮離子電 流的影響,括弧內為樣本數。具止瀉功能之中草藥黃連、鴉膽子、秦 皮、苦參、黃柏酒精抽出濃縮物質均則可使接受 forskolin 刺激之大白 鼠腸道上皮短路電流下降。具瀉下功能之中草藥酒精抽出濃縮物質則 可使接受 forskolin 刺激之大白鼠腸道上皮短路電流上升。
圖七、鈉離子與氯離子移動與秦皮酒精抽出物影響腸道上皮短路電流 之關係。接受 forskolin 刺激大白鼠腸道上皮之短路電流之後分別給予 ouabain 及 bumetanide 處理，再加入秦皮酒精抽出物，腸道上皮短路 電流變化值。以 ouabain 處理，再加秦皮酒精抽出物，短路電流變化 值與對照組差異不甚明顯，以 bumetanide 處理，再加秦皮酒精抽出 物，短路電流變化值對照組則有明顯差異。（n=6），*p＜0.05 v.s control。
圖八、鈉離子與氯離子移動與黃連酒精抽出物影響腸道上皮短路電流 之關係。以 ouabain 處理，再加黃連酒精抽出物，短路電流變化值為 與對照組差異不甚明顯（p=0.187）（n=6）。以 bumetanide 處理，再加 黃連酒精抽出物，短路電流變化值也與對照組（p=0.09）差異不明顯。
（n=6）。
圖九、鈉離子與氯離子移動與苦參酒精抽出物影響腸道上皮短路電流 之關係。以 ouabain 處理，再加苦參酒精抽出物，短路電流變化值對 照組有明顯差異。以 bumetanide 處理，再加苦參酒精抽出物，短路電 流變化值與對照組差異不甚明顯（n=6）。*p＜0.05 v.s control。
圖十、鈉離子與氯離子移動與大黃酒精抽出物影響腸道上皮短路電流 之關係。以 ouabain 處理，再加大黃酒精抽出物，短路電流變化值與 對照組（14.33 ±3.07µA）差異明顯。以 bumetanide 處理，再加大黃 酒精抽出物，短路電流變化值與對照組也有明顯差異。（n=6），*p＜
0.05 v.s control。
圖十一、鈉離子與氯離子移動與巴豆酒精抽出物影響腸道上皮短路電 流之關係。以 ouabain 處理，再加巴豆酒精抽出物，短路電流變化值 與對照組差異明顯。以 bumetanide 處理，再加巴豆酒精抽出物，短路 電流變化值與對照組也有明顯差異。（n=6），*p＜0.05 v.s control。
圖十二、鈉離子與氯離子移動與火麻仁酒精抽出物影響腸道上皮短路 電流之關係。以 ouabain 處理，再加火麻仁酒精抽出物，短路電流變 化值與對照組)差異明顯（p=0.003）。以 bumetanide 處理，再加火麻 仁酒精抽出物，短路電流變化值，與對照組也有明顯差異。（n=6），
*p＜0.05 v.s control。
圖十三、苦參酒精抽出物對鈉離子移動的影響。接受 forskolin 刺激大 白鼠腸道上皮，給予苦參酒精抽出物，結果顯示在加入苦參之後，短 路電流明顯下降，再以 ouabain 處理，短路電流變化值再度下降，平 均下降值與對照組(接受 forskolin 刺激之後給予 ouabain) 電流平均值 下降值差異明顯。（n=4），*p＜0.05 v.s control。
圖十四、秦皮酒精抽出物對氯離子移動的影響
。
接受 forskolin 刺激 大白鼠腸道上皮，給予秦皮酒精抽出物，結果顯示在加入秦皮之後，短路電流明顯下降，再以 bumetanide 處理之後，短路電流變化值再度 下降，與對照組(接受 forskolin 刺激之後給予 bumetanide) 電流平均 下降值差異明顯。（n=4），*p＜0.05 v.s control。