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表格與圖片
表一. UBQ10、UBQ11 和 UBQ14 基因之啟動子作一連串刪除使用引子資料表(一) 名稱 限制酵素切點 序列(5'-3')
UBQ14-F2 Kpn I GGGGTACCCACCATCCCATATTAATATCGATTC UBQ14-F3 Kpn I GGGGTACCCATGCTCATTCGTAATCGAAATC UBQ14-F4 Kpn I GGGGTACCGCGATGTGGGACATTGTACAC UBQ14-F5 Kpn I GGGGTACCCAATTGTATTAATGCTTCTATTCTGG UBQ14-F6 Kpn I GGGGTACCGGATTTAACTCCTAGTTCTATCGC UBQ14-F7 Kpn I GGGGTACCCATACTATCCCATGTTATTATCCAC UBQ14-F8 Kpn I GGGGTACCCTAAGATTTCTCTCGCAGTATATC UBQ14-F9 Kpn I GGGGTACCCCAAACCCGTCCTACCATATAAC UBQ14-F10 Kpn I GGGGTACCCAATATCGACCCAAAATTCATCATTG UBQ14-F11 Kpn I GGGGTACCGATTTTTCCTATAATATATCGTCTCG UBQ14-F12 Kpn I GGGGTACCTCCGAGTAAAATCGGATAACTATT UBQ14-F13 Kpn I GGGGTACCCCAGGATCCGAACAGAGTTAAAC
UBQ14-F14 Kpn I GGGGTACCATTATCTTCAAAAACCTCCTACAAAAATG UBQ14-F15 Kpn I GGGGTACCCATGGGTAACGGTTCAATATGATC
UBQ14-F16 Kpn I GGGGTACCGCTCTAATAACCGGGACGATAG UBQ14-F17 Kpn I GGGGTACCGTAAAAGTTACGTGTCAAGCTTTG UBQ14-F18 Kpn I GGGGTACCCCTAAGACCTATTCTTCTTCACA UBQ14-R2 Sal I CGCGTCGACCTTGATCCCGATTGAGAGATTTGA UBQ14-R3 Sal I CGCGTCGACCTGTAATTCACAAAAAACTGAGA UBQ14-R4 Sal I CGCGTCGACACCACCACGGAGCCTGAGAAC UBQ11-F2 Kpn I GGGGTACCCGCCATTTTCAATATCACGGTCC UBQ11-F3 Kpn I GGGGTACCCAGTACCAAAGAGTTGCTTCATG
UBQ11-F4 Kpn I GGGGTACCGAACTTGATTGGGATCATTTACTG UBQ11-F5 Kpn I GGGGTACCCTGAATGTCCACCTGAATCCTTG
UBQ11-F16 Kpn I GGGGTACCGAATGGGTGAGAGACGAATGCC UBQ11-F17 Kpn I GGGGTACCGTGTGATACTGATGTTAACTGTG UBQ11-F18 Kpn I GGGGTACCGTAGACGCTGCTTGAAACTTAAAG
表一. UBQ10、UBQ11 和 UBQ14 基因之啟動子作一連串刪除使用引子資料表(二) 名稱 限制酵素切點 序列(5'-3')
UBQ11-F19 Kpn I GGGGTACCCCGAGTCAAAATGTGTTGTGTG UBQ11-F20 Kpn I GGGGTACCGATTTATCTGCATCAAATGCTTTC UBQ11-F21 Kpn I GGGGTACCCACTTGTGAACCTTACACAAAGG
UBQ11-F22 Kpn I GGGGTACCGACAAACAAAACCCATCTCTTTCAG UBQ11-F23 Kpn I GGGGTACCGATCTCATCTAATGGTAATTCAAC
UBQ11-F24 Kpn I GGGGTACCCAACTTTGCGTGTGAACAACGC UBQ11-R2 Sal I CGCGTCGACCTTGATCACGGTTGAGAGATTTG UBQ11-R3 Sal I CGCGTCGACCTGTTAATCAGAAAAACCGAGA UBQ10-F2 Kpn I GGGGTACCCTTCTGGCCCTTAAGTCACAATG UBQ10-F3 Kpn I GGGGTACCGAATCGGGATAATGACAGCACAG UBQ10-F4 Kpn I GGGGTACCGAGACTTGTTCACCAACTTGATAC UBQ10-R2 Cla I CCATCGATCTTGATCACGGTAGAGAGAATTG UBQ10-R3 Cla I CCATCGATCTGTTAATCAGAAAAACTCAGAT UBQ10-R4 Cla I CCATCGATACCACCACGGAGCCTGAGGA
UBQ10-R5 Cla I CCATCGATAATCTGCATACCACCACGGAGCCTGAGGA UBQ10-F5 Kpn I GGGGTACCGTGGATTTTATCCTATTTGTAGGTG
UBQ10-F6 Kpn I GGGGTACCCAGCGATCAAGCTTCTCTTATAAG UBQ10-F7 Kpn I GGGGTACCGAAATCTCATTGCAACATCAAATGG UBQ10-F8 Kpn I GGGGTACCGGCTCAACAACAAACTTTCCATTC UBQ10-F9 Kpn I GGGGTACCGAATACAACTTTAGATCATAATTCTC UBQ10-F10 Kpn I GGGGTACCGATACTTTATGTCTTGGATAATTGG UBQ10-F11 Kpn I GGGGTACCCATAACACCTTCCCTGTATGATC UBQ10-F12 Kpn I GGGGTACCGTGGTGGAGCTTCCCATGTTTC UBQ10-F13 Kpn I GGGGTACCCCTTACTAATACCTCATTGGTTCC UBQ10-F14 Kpn I GGGGTACCGGACTAAAGCCTCCACATTCTTC UBQ10-F15 Kpn I GGGGTACCCATAGCGAACTTATTCAAAGAATG UBQ10-F16 Kpn I GGGGTACCGGACCAGGCCCCAAATAAGATC UBQ10-F17 Kpn I GGGGTACCCACAATATGTTATACGATAAAGAAG UBQ10-F18 Kpn I GGGGTACCACGAAATACGCTTCAATGCAGTG UBQ10-F19 Kpn I GGGGTACCCGTAACGATCGTTAAATCTCAACG UBQ10-F20 Kpn I GGGGTACCGCACACACGAGTCGTGTTTATC
表二. RT-PCR實驗所使用的引子資料表
名稱 序列(5'-3') 預測放大序列長度
UBQ14-F1 TTTCGTGAAGACTCTCACTGG
UBQ14-R1 ACTTCTTCTGATTCATAACAGAGATAA 256 bp UBQ11-F1 CACTCTTGAAGTTGAGAGCTC
UBQ11-R1 ATACATGAACTTGGTTCAGTAACC 240 bp UBQ10-F1 CTTCGTCAAGACTTTGACCG
UBQ10-R1 CTTCTTAAGCATAACAGAGACGAG 251 bp UBQ5-F1 GGTGGTGCTAAGAAGAGGAAGA
UBQ5-R1 GATCAAGCTTCAACTCCTTCTT 254 bp PP2A F1 GTGGCCAAAATGATGCAATCTC
PP2A R1 CAATGGTCACGTACGTGTAAAG 270 bp eIF-1A-1F GATAATAATGATGATGACTATGTTG eIF-1A-1R CTTATGCAACAAGAACACCAAG 217 bp CBF2-F1 GACGTGTCCTTATGGAGCTATTA
CBF2-R1 CCATTTACATTCGTTTCTCACAAC 131 bp
表三. 阿拉伯芥泛素基因之微陣列資料分析
取自於GENEVESTIGATOR資料庫的微陣列資料，可分為在不同突變株(mutant) 和接受不同環境處理(stimulus)之後，各基因的表現情況，以1為標準植。將取 得的數值計算平均值(mean)以及標準誤(standard error)，平均值愈趨近於1，標 準誤愈小，則表示此基因的表現愈穩定。
Name Accession Number Mutant Mean ± Standard Error
Stimulus Mean ± Standard Error UBQ3 BAH19791 1.25± 0.09 1.2± 0.06
UBQ4 BAF00213 1.08± 0.024 1.07±0.023 UBQ10 CAB81074 1.03± 0.014 1.01± 0.012
UBQ11 CAB81047 1.11± 0.04 1.14± 0.03 UBQ14 BAH19554 1.02± 0.02 1.11± 0.025
UBQ1 AAK59652 1.00± 0.01 1.00± 0.013
UBQ2 AAM15407 1.01± 0.016 0.97±0.017 UBQ5/UBQ6 BAE98979/AAC34235 1.00± 0.009 1.01±0.014
UBQ5/6 like ABD59101 1.15±0.06 1.02±0.052
表四. UPE1、UPE2以及UPE3序列在PLACE比對出的已知順式作用元素
由MEME比對出UBQ10/UBQ11/UBQ14啟動子上的保守序列，再比較PLACE資 料庫中已知的順式作用元素序列。
UPE1 Factor or Site Name Loc.(Str.) Signal Sequence
CAATBOX1 7 (-) CAAT
CBFHV 35 (+) RYCGAC
DOFCOREZM 25 (-) AAAG DRE2COREZMRAB17 35 (+) ACCGAC DRECRTCOREAT 35 (+) RCCGAC GTGANTG10 32 (+) GTGA GTGANTG10 13 (-) GTGA LTRECOREATCOR15 36 (+) CCGAC
MYB1AT 2 (-) WAACCA
POLLEN1LELAT52 26 (-) AGAAA REALPHALGLHCB21 1 (-) AACCAA RHERPATEXPA7 30 (-) KCACGW WBOXNTERF3 33 (+) TGACY
WRKY71OS 33 (+) TGAC
UPE2 CAATBOX1 9 (+) CAAT DOFCOREZM 26 (-) AAAG SEF4MOTIFGM7S 17 (-) RTTTTTR TATABOX5 1 (-) TTATTT TBOXATGAPB 25 (+) ACTTTG UPE3 ASF1MOTIFCAMV 4 (-) TGACG
CACTFTPPCA1 10 (-) YACT
DOFCOREZM 8 (+) AAAG
TBOXATGAPB 7 (-) ACTTTG WBOXATNPR1 5 (-) TTGAC
WRKY71OS 5 (-) TGAC
U 的E2 結合使泛素鍵結到蛋白受質(substrate)上。參與在不同生理反應的受質常 鍵結不同種形式的Ub 或是 Ub 鏈。 表示為 Ub protein。 U
SF
UBQ10/UBQ11/UBQ14 延伸胺基酸為苯丙胺酸為第二群。Ub 表示為 ubiquitin coding sequence、S 為絲胺酸(serine)、F 為苯丙胺酸(phenylalanine)。
圖三. UBQ11、UBQ11 和 UBQ14 的基因表現
由GENEVESTIGATOR 網站所得之資料，在阿拉伯芥中的每個生長時期，都 以UBQ10 的基因表現量為最高。圖示 UBQ3(●)、UBQ4(●)、UBQ10(●)、
UBQ11(●)and UBQ14(●)。
A
Promoter region intron Ub 5’UTR
Promoter region intron Ub nos 5’UTR
LUC
nos LUC
LUC nos Promoter region intron
5’UTR
Promoter region 5’UTR
nos Promoter region LUC
GUS 35S promoter nos GUS 35S promoter nos
GUS 35S promoter nos GUS 35S promoter nos
GUS 35S promoter nos GUS 35S promoter nos
GUS 35S promoter nos GUS 35S promoter nos
圖四. 聚泛素基因的基因結構以及不同組合的調節序列質體構築構築
A. 聚泛素基因的共同結構次序是啟動子序列，5’端非轉譯區，唯一的內插 子，以及緊接在內插子後的Ub 編碼序列(coding sequence)。
B. 組合上游序列的不同區段，並接上 LUC 報導基因。在同一質體內亦含有 CaMV 35S 啟動子所驅動的 GUS 作為控制組。
Promoter region intron Ub (-2044 to +389)
pUB10-1
+1
pUB10-2 (-1948 to +389)
(-1849 to +389) pUB10-3
(-1747 to +389) pUB10-4
(-1434 to +389) pUB10-5
LUC LUC (-118 to +389)
pUB10-7(-731 to +389) pUB10-6
pUB10-8 pUB10-9
pUB10-10
(-2044 to +617) (-1948 to +617)
(-1849 to +617)
+84 +389 轉譯區以及內插子序列，pUB10-8 到 pUB10-10 為不同長度啟動子序列加上 5’端非轉譯區、內插子序列以及一個 Ub 編碼序列。
C. UBQ10 啟動子活性分析，計算 LUC/GUS 比值與標準誤差(standard error)，
其中以GUS 活性為控制組。每次實驗均重複三次。
A B
C
Promoter region intron (-1046 to +426)
(-557 to +426)
Promoter region intron
UPE1：TTGGTTATTGCTTCACCGCCTTAGCTTTCTCGTGACCGACTCGTCCTCG
UBQ14 TGG TTGGTTATTGCTTCACCGCCTTAGCTTTCTCGTGACCGACTCGTCCTCG TCT UBQ10 TTA TTAGCTATTGCTTCACCGCCTTAGCTTTCTCGTGACCTAGTCGTCCTCG TCT UBQ11 TAA TTGGCTATTGCTTCCACGCCTTAGCTTTCTCGTGACCGACCGAGTCGTC CTC
5’ 3’
(-1962 to -1913)
(-2366 to -2317)
(-2034 to 1985)
圖八. UBQ10, UBQ11 和 UBQ14 啟動子調控序列 1(UPE1)之序列及其相似度和位置 A. MEME比對出的UPE1序列。
B. 使用Weblogo排出UPE1的相似度，字形越大代表越保守。
C. UPE1在UBQ10, UBQ11和UBQ14中的相對位置。