第四節 第四節
第四節 大豆蛋白對於糞便中性固醇與膽酸排出量的影響大豆蛋白對於糞便中性固醇與膽酸排出量的影響 大豆蛋白對於糞便中性固醇與膽酸排出量的影響大豆蛋白對於糞便中性固醇與膽酸排出量的影響
先前的研究指出，大豆蛋白降低血漿膽固醇濃度的可能機制之一，
是由於大豆蛋白在腸道中扮演膳食纖維的角色，與膽酸結合、促進糞便 中膽酸的排出，加速肝臟中膽固醇再合成膽酸的速率，進而降低血漿膽 固醇濃度 (Chen et al., 2003)。然而，由本次的實驗結果指出，飲食中 大豆蛋白的攝取，並不會增加糞便中中性固醇與膽酸的排出，但仍可顯 著降低血漿與肝臟膽固醇濃度及三酸甘油酯濃度，因此推測，市售大豆 蛋白降低血脂質的作用機轉並非藉由促進脂質的排出，而是降低脂質的 合成，但仍需進一步的研究來加以證實。
第五章 第五章 第五章
第五章 總結總結總結總結
飲食中以 50% 大豆蛋白取代酪蛋白，可降低血漿膽固醇與肝臟膽 固醇濃度及三酸甘油酯濃度。大豆蛋白食用量經換算，體重 60 公斤成 人每天攝取 25 公克大豆蛋白，即可達到降低血膽固醇的效果。認為可 進一步應用於心血管疾病的預防。
參考文獻
New Engl J Med 333:276-282.
Anderson JJ, Anthony MS, Cline JM, Washburn SA and Garner SC (1999) Health potential of soy isoflavones for menopausal women.
Publ Health Nutr 2:489-504.
Ascencio C, Torres N, Isoard-Acosta, Gόmez-Pérez FJ, Hernández-Pando R and Tovar AR (2004) Soy protein affects serum insulin and hepatic SREBP-1 mRNA and reduces fatty liver in rats. J Nutr 134:522-529.
Bakhit RM, Klein BP and Essex-Sorlie D (1994) Intake of 25 g of soybean protein with or without soybean fiber alters plasma lipids in men with elevated cholesterol concentrations. J Nutr 124:213-222.
Banz Wj, Davis J, Peterson R and Iqbal MJ (2004) Gene expression and adipocity are modified by soy protein in male Zucker diabetic fatty rat. Obes. Res. 12:1907-1913.
Bodwell CE (1979) Human versus animal assays. In: Wilcke HL, Hopkins DT, Waggle DH, eds. Soy protein and human nutrition.
New York: Academic Press 121-143.
Bodwell CE, Satterlee LD and Hackler LR (1980) Protein digestibility of the same protein preparations by humans and rat assays and by in vitro enzymic digestion methods. J Nutr 33:677-686.
Carroll KK, Huff MW and Roberts DCK (1979) Vegetable protein and
lipid metabolism. In: Wilcke HL, Hopkins DT, Waggle DH, eds. Soy Protein and Human Nutrition. New York, NY: Acadmic Press;
261-280.
Carroll KK and Kurowska EM (1995) Soy consumption and cholesterol reduction: Review of animal and human studies. J Nutr 125:594s-597s.
Chen Z, Peto R, Collins R, MacMahon S, Lu J and Li W (1991) Serum cholesterol and coronary heart disease in population with low cholesterol concentration. BMJ 303:276-282.
Chen JR, Chiou SF, Suetsuna K, Yang HY and Yang SC (2003) Lipid metabolism in hypercholesterolemic rats affected by feeding cholesterol-free diets containing different amounts of non-dialyzed soybean protein fraction. Nutrition 19:676-680.
Davis MJ, Krikler DM and Katz D (1991) Atherosclerosis: Inhibition or regression as therapeutic possibilities. Br Heart J 65:302-310.
Davis JN, Singh B, Bhuiyan M and Sarkar FH (1998) Genistein-induced upregulation of p21WAF1, downregulation of cycline B, and induction of apoptosis in prostate cancer cells. Nutr Cancer 32:123-131.
Dobiasova M and Frohlich J (2001) The plasma parameter log (TG/HDL-C) as an atherogenic index: correlation with lipoprotein particle size and esterification rate in apoB-lipoprotein-depleted plasma (FER(HDL)). Clin Biochem 34:583-588.
Erdman JW (2000) Soy protein and cardiovascular disease.
Circulation 102:2555-2559.
the isolation and purification of total lipids form animal tissues. J Biol Chem 226:497-509.
Food and Drug Administration, (1999) Food labeling, health claims, soy protein, and coronary heart disease. Fed Reg 57:699-733.
Forsythe Ⅲ WA (1986) Comparison of dietary casein or soy protein effects on plasma lipids and hormone concentrations in the gerbil (Meriones unguiculatus) J Nutr 116:1165-1171.
Forsythe Ш WA (1990) Dietary proteins, cholesterol and thyroxine: A propose mechanism. J Nutr Sci Vitaminal 36:101-104.
Gaddi A, Descovich GC, Noseda G, Fragiacomo C, Nicolini A, Montanari G, Vanetti G, Sirtori M, Gatti E and Sirtori CR (1987) Hypercholesterolemia treated by soybean protein diet. Arch Dis Child 62:274-278.
Greaves KA, Wilson MD, Rudel LL, Williams JK and Wagner JD (2000) Consumption of soy protein reduces cholesterol absorption compared to casein protein alone or supplemented with isoflavone extract or conjugated estrogen in ovariectomized cynomologous monkeys. J Nutr 130: 820-826.
Grundy SM, Ahrens EH and Miettinen TA (1965) Quantitative isolation and gas-liquid chromatographic analysis of total fecal bile acids. J Lipid Res 6: 397-410.
Hegsted D (1986) Serum-cholesterol response to dietary cholesterol:
a re-evaluation. Am L Clin Nutr 44:299-305.
Hodgson JM, Croft KD, Puddey IB, Mori TA and Beilin LJ (1996) Soybean isoflavanoids and their metabolic products inhibit in vitro
lipoprotein oxidation in serum. J Nutr Biochem 7:664-669.
Honore EK, Williams JK, Anthony MS and Clarkson TB (1997) Soy isoflavones enhance coronary vascular reactivity in atherosclerotic female macaques. Fertil Steril 67: 148-64
Huff MW, Hamilton RMG and Carroll KK (1977) Plasma cholesterol levels in rabbits fed low fat, cholesterol-free semipurified diets.
Effects of dietary proteins, protein hydrolysates and amino acid mixtures. Atherosclerosis 28:187-195.
Huff MW, Roberts DCK and Carroll KK (1982) Long-term effects of semipurified diets containing casein or soy protein isolate on atherosclerosis and plasma lipoproteins in rabbits. Atherosclerosis 41:327-336.
Iritani N, Suga A, Fukuda H, Katsurada A and Tanaka T (1988) Effects of dietary casein ad soybean protein on triglyceride turnover in rat liver. J Nutr Sci Vitaminol 34:309-315.
Kritchevsky D (1995) Dietary protein, cholesterol and atherosclerosis:
A review of the early history. J Nutr 125: 598s-593s.
Lin DS and Connor WE (1980) The long term effects of dietary cholesterol upon the plasma lipids, lipoproteins, cholesterol absorption, and the sterol balance in man: the demonstration of feedback inhibition of cholesterol biosynthesis and increased bile acid excretion. J Lipid Res 21:1042-1052.
Lin Y, Meijer GW, Vermeer MA and Trautwein EA (2004) Soy protein enhances the cholesterol-lowering effect of plant sterol esters in cholesterol-fed hamsters. J Nutr 134:143-148.
Coronary Primary Prevention Trial Results. II. The relationship of reduction in incidence of coronary heart disease to cholesterol lowering. J Am Med Assoc 251:365-374.
Meeker DR and Kesten HD (1941) Effect of high protein diet on experimental atherosclerosis of rabbits. Arch Pathol 31:147-162.
Mokady S and Liener IE (1982) Effect of plant proteins on cholesterol metabolism in growing rats fed atherogenic diets. Ann Nutr Metab 25:138-144.
Morita T, Oh-hashi A, Takei K, Ikai M, Kasaoka S and Kiriyama S (1997) Cholesterol-lowering effects of soybean, potato and rice proteins depend on their low methionine contents in rats fed a cholesterol-free purified diet. J Nutr 127:470-477.
Mukhin DN, Orekhov AN, Andreeva ER, Schindeler EM and Sminov VN (1991) Lipids in cells of atherosclerotic and uninvolved human aorta. III. Lipid distribution in intimal sublayers. Exp Mol Pathol 54:22-30.
Murray RK, Granner DK, Mayes PA and Rodwell VW (1993) Cholesterol synthesis, transport and excretion. In: Harper’s Biochemistry. P266-279. Mayes, P.A. ed. Lange Medical Publications. Connecticut.
Nagata Y, Ishiwaki N and Sugano M (1982) Studies on the mechanism of the antihypercholesterolemia action of soy protein and soy protein-type amino acid mixtures in relation to their casein counterparts in rats. J Nutr 112: 1614-1625.
Nagata C, Tanaka K and Sugano M (1981) Further studies on the hypocholesterolemic effect of soybean protein in rats. Br J Nutr.
45:233-241.
Ness GC, Pendleton LC, Li YC and Chiang JYL (1990) Effect of thyroid hormone on hepatic cholesterol 7α-hydroxylase, LDL receptor, HMG-CoA reductase, farnesyl pyrophosphate synthase and apolipoprotein A-I mRNA levels in hypophysectomized rats.
Biochem Biophys Res Commun 172:1150-1156.
Ogawara H, Akiyama T and Watanabe S (1989) Inhibition of tyrosine protein kinase activity by synthetic isoflavones and flavones. J Antibiotic 42:340-343.
Peng SK, Tham P, Taylor CN and Mikkelson B (1979) Cytotoxicity of oxidation derivatives of cholesterol on cultures aortic smooth muscle cells and their effect on cholesterol biosynthesis. Am J Clin Nutr 32:1033-1042.
Potter SM (1995) Overview of proposed mechanisms for the hypocholesterolemic effects of soy. J Nutr 125:606s-611s.
Potter SM (1996) Soy protein and serum lipids. Curr Opin Lipidol 7:260-264.
Reeves PG, Nielsen FH and Fahey GC (1993) AIN-93 Purified diets for laboratory rodents: Final report of the American Institute of Nutrition ad hoc writing committee on the reformulation of the AIN-76A rodent diet. J Nutr 123:1939-1951.
Roberts WC (1995) Preventing and arresting coronary atherosclerosis.
Am Heart J 130(3) Pt 1:580-600.
Rodwell VW, Nordstrom JL and Mitschelen JJ (1976) Regulation of HMG-CoA reductase. Adv Lipid Res 14:1-74.
insulin/glucagons ratio as an explanation for the dietary protein modulation of atherosclerosis. Med Hypotheses 35:324-329.
Sanchez S and Hubbard RW (1989) Dietary protein modulation of serum cholesterol: the amino acid connection. In Absorption and Utilization of Amino Acids; Friedman M, Ed.; CRC, Boca Raton:FL;
Vol. 3, pp 247-273.
Sarwar G, Peace RW, Botting HG (1985) Corrected relative net protein ratio (CRNPR) method based on differences on rat and human requirements for sulfur amino acids. J Asso Official Ana Chem 68:689-693.
Sautier C, Flament C, Doucet C and Suquet JP (1986) Effect of eight dietary proteins and their amino acid content on serum, hepatic and fecal steroids in the rat. Nutr Rep Int 34:1051-1059.
Sirtori CR. Professor CR. Sirtori replies. (1983) Trends Pharmacol Sci 4:63. Letter.
Sirtori CR, Pazzucconi F, Colombo L, Battistin P, Bondioli A and Descheemaeker K (1999) Double-blind study of the addition of high-protein soya milk v. cows’ milk to the diet of patients with severe hypercholesterolemia and resistance to or intolerance of statins. Br J Nutr 82:91-96.
Stange EF, Schneider MAA, Ditschuneit H and Poley JR (1981) Influence of dietary cholesterol, saturated and unsaturated lipid on 3-hydroxy-3-methylglutaryl-CoA reductase activity in rabbit intestine and liver. J Lipid Res 22:47-56.
Sugano M, Yamada Y, Yoshida K, Hashimoto Y, Matsuo T and Kimoto M (1988) The hypocholesterolemic action of the undigested
fraction of soybean protein in rats. Atherosclerosis 72:115-122.
Teixeira SR, Potter SM, Weigel R, Hannum S, Erdman JW Jr and Hasler CM (2000) Effects of feeding 4 levels of soy protein for 3 and 6 wk on blood lipids and apolipoproteins in moderately hypercholesterolemia men. Am J Clin Nutr 71:1077-1084.
Terpstra AHM, Harkes L and van der Veen FH (1981) The effect of different proportions of casein in semipurified diets on the concentration of serum cholesterol and the lipoprotein composition in rabbits. Lipids 16:114-119.
Torun B, Viteri FE and Young VR (1981) Nutritional role of soya protein for humans. J Am Oil Chem Soc 58:400-406.
Wang W, Higuchi CM and Zhang R (1997) Individual and combinatory effects of soy isoflavones on the in vitro potentiation of lymphocyte activation. Nutr Cancer 29:29-34.
Wayler A, Queiroz E, Scrimshaw NS, Steinke FH, Rand WM and Young VR (1983) Nitrogen balance studies in young men to assess the protein quality of an isolated soy protein in relation to meat proteins. J Nutr 113:2485-2491.
Weihua N, Yasuyuki T, Masanobu S and Katsumi I (1998) Dietary soy protein isolate, compared with casein, reduces atherosclerotic lesion area in apolipoprotein E-deficient mice. J Nutr 128:1884-1889.
Wong WW, Smith EO, Stuff JE, Hachey DL, Heird WC and Pownell HJ (1998) Cholesterol-lowering effects of soy protein in normocholesterolemic and hypercholesterolemic men. Am J Clin
Yashiro A, Oda S and Sugano M (1985) Hypocholesterolemic effect of soya protein for humans. J Am Oil Chem Soc 58: 400-406.
Young VR (1991) Soy protein in relation to human protein and amino acid nutrition. J Am Diet Assoc 91:828-835.
Zezulka AY and Calloway DH (1976) Nitrogen retention in men fed varying levels of amino acids from soy protein with or without added L-methionine. J Nutr 106:212-221.
表 一、各組飼料組成百分比 (%)
Table1. The composition of hamster diets (%)
H S1 S2
1Corn starch: ASMYANG GENEX CORP.
2Casein: ICN-901293
3Soy protein: MIKI Protein 95
4Mineral mixture: ICN-905455
5Cellulose: ICN-900453
6Vitamin mixture: ICN-905454
7Cholesterol: SIGMA C8503
8Methionine: ICN-190955
9Choline: SIGMA C1629
10Chloic acid: SIGMA C1254
表 二、不同飼料對倉鼠體重與攝食量的影響
Table 2. Body weight and food intake of hamsters fed different diets
H S1 S2
Weight (g) 96.2 ± 5.6 99.2 ± 7.6 99.0 ± 5.1
Food intale (g/day) 8.4 ± 1.2 8.6 ± 0.9 8.9 ± 1.9
Values are presented as the mean ± SD (n=8).
abcValues in a row with different letters of superscript mean significant difference (p < 0.05).
表 三、不同飼料對倉鼠血脂與肝功能指數的影響 Table3. Plasma profiles of hamsters fed different diets
H S1 S2
Plasma
Total cholesterol (mmol/L) 6.2 ± 0.6a 6.1 ± 0.3a 3.9 ± 0.2b
Triglyceride (mmol/L) 1.0 ± 0.2 1.0 ± 0.4 0.7 ± 0.2
LDL-C (mmol/L) 3.0 ± 0.5a 3.1 ± 0.3a 1.5 ± 0.2b
HDL-C (mmol/L) 2.7 ± 0.2a 2.7 ± 0.2a 1.6 ± 0.2b
GOT (IU/L) 20.6 ± 8.9 22.6 ± 3.2 19.3 ± 6.9
GPT (IU/L) 51.0 ± 21.0 52.2 ± 9.3 66.2 ± 22.8
Values are presented as the mean ± SD (n=8).
abcValues in a row with different letters of superscript mean significant difference (p < 0.05).
表四、不同飼料對倉鼠肝重及肝中脂質濃度的影響
Table4. Liver weight and liver lipids concentration of hamsters fed different diet
H S1 S2
Liver
Liver weight 6.5 ± 0.9a 6.1 ± 0.5a 3.6 ± 0.8b
Cholesterol (µmol/liver) 0.6 ± 0.0a 0.6 ± 0.1a 0.2 ± 0.0b
Triglyceride (µmol/liver) 1.0 ± 0.5a 0.8 ± 0.2a 0.5 ± 0.1b
Values are presented as the mean ± SD (n=8).
abcValues in a row with different letters of superscript mean significant difference (p < 0.05).
表 五、不同飼料對倉鼠糞便中性固醇與糞便膽酸的影響
Table5. Fecal neutral steroids and bile acid excretion of hamsters fed different diet
H S1 S2
Fecal
Neutral steroids (µmol/day) 8.17 ± 0.59 9.81 ± 2.06 7.54 ± 0.77
Bile acid (µmol/day) 0.15 ± 0.01 0.17 ± 0.01 0.12 ± 0.00
Values are presented as the mean ± SD (n=8).
abcValues in a row with different letters of superscript mean significant difference (p < 0.05).