磁熱應答水膠相轉變行為分析

分具有相轉變的性質。Sample 25A 和 sample 25D 可發現到具有和未 加磁性粒子的 CFC 共聚物一樣的 sol-gel-sol 的特性。由實驗結果可得 知，我們改變粒子濃度時，水凝膠的相轉變特性會受到粒子含量多寡

表 7、不同比例的 Pluronic、磁性奈米粒子和 DI 水組成的水凝膠之 相轉變圖。

4.3.2 以 CoFe

O

@ DCFCD 及 CFC 製備磁熱水膠之分析

列成膠態。因此可以歸納出，當 F127 高分子濃度上升時，LCST 會 下降。而 CFC 高分子水膠因為末端接枝疏水的 PCL 片段，疏水性較 強，在高分子濃度 25 wt%時與 F127 水膠相比較易形成 gel，且因末 端接枝 PCL，使高分子本身的亂度上升，導致高分子濃度對 LCST 的 影響較小， 25 wt%與 30 wt%的 CFC 水膠相比，LCST 的改變皆不明 顯。

表 8、CoFe2O4@DCFCD 與不同濃度的 CFC 組成的水膠之相轉變圖

表 9、CoFe2O4@DFD 與不同濃度的 F127 組成的水膠之相轉變圖

第五章、結論

5. 熱敏性高分子以化學鍵結方式接枝於鈷鐵氧化物粒子上，使 的粒子可以均勻分散在高分子溶液中，因此不會產生沉澱而 影響高分子本身的成膠性質，也解決了磁性水膠一開始相分 離的問題。

6. F127 高分子濃度上升時，LCST 會下降。而 CFC 高分子水 膠因末端接枝疏水的 PCL 片段，疏水性較強，在高分子濃 度 25 wt%時與 F127 水膠相比有較低的 LCST (19.7℃)，且 因末端接枝 PCL，使高分子本身的亂度上升，導致高分子濃 度對 LCST 的影響較小，CFC 水膠於 25 wt%與 30 wt%時具 有相近的 LCST。

7. 未來，磁性敏感塊狀共聚水膠經過細胞相容性測試後，可藉 由磁場控制溫度，使溫敏性水膠的 sol-gel 相變化，應用於 藥物釋放。

第六章、參考文獻

Arruebo, M., Fernández-Pacheco, R., Ibarra, M. R. and Santamaría, J.

(2007) Magnetic nanoparticles for drug delivery. Nano Today. 2, 22-32.

Alexander, V. K., Elena V. B. and Valery Y. A., (2002) Pluronic block copolymers as novel polymer therapeutics for drug and gene delivery.Journal of Controlled Release .82,189-212.

Anna, G., Jong, S.B., Ick, C. K., You, H. B., Younsik, C. and Sung, W.

K., (1997) Squeezing hydrogels for controlled oral drug delivery.

Journal of Controlled Release.48,141-148.

Byeongmoon J., Sung W. K. and You H. B., (2002) Thermosensitive sol–gel reversible hydrogels. Advanced Drug Delivery Reviews. 54, 37-51.

Cai, K. Y., Luo, Z., Hu, Y., Chen, X. Y., Liao, Y. J., Yang, L. and Deng, L. H. (2009) Magnetically triggered reversible controlled drug

delivery from microfabricated polymeric multireservoir devices. Adv.

Mater. 21, 4045-4049.

Chang, C. L., Yi, Y. G., Yang, F. P., , Ji, D. Z., Wei, W., Mei, J. H.,

Yong, S. W., Ke, W., Ma, L. G., Ming, J. T., Yu, Q. W. and Zhi,Y. Q., (2007) Synthesis and characterization of a thermosensitive hydrogel based on biodegradable amphiphilic PCL-Pluronic (L35)-PCL block copolymers. Colloids and Surfaces. 302 ,430-438.

Chatterjee, J., Haik, Y. and Chen, C. J. (2003) Biodegradable magnetic gel: synthesis and characterization. Colloid Polym. Sci. 281, 892-896.

Chen, M. C., Liu, C. T., Tsai, H. W., Lai, W. Y., Chang, Y. and Sung, H.

W. (2009) Mechanical properties, drug eluting characteristics and in vivo performance of a genipin-crosslinked chitosan polymeric stent.

Biomaterials 30, 5560-5571.

Corchero, J. L. and Villaverde, A. (2009) Biomedical applications of

Daou, T. J., Pourroy, G., Bégin-Colin, S., Grenèche, J. M.,

Ulhaq-Bouillet, C., Legaré, P., Bernhardt, P., Leuvrey, C. and Rogez, G. (2006) Hydrothermal synthesis of monodisperse magnetite

nanoparticles. Chem. Mat. 18, 4399-4404.

Frey, N. A., Peng, S., Cheng, K. and Sun, S. (2009) Magnetic nanoparticles: synthesis, functionalization, and applications in bioimaging and magnetic energy storage. Chem. Soc. Rev. 38, 2532-2542.

Furukawa, H., Shimojyo, R., Ohnishi, N., Fukuda, H. and Kondo, A.

(2003) Affinity selection of target cells from cell surface displayed libraries: a novel procedure using thermo-responsive magnetic nanoparticles. Appl. Microbiol. Biotechnol. 62, 478-483.

Grief, A. D. and Richardson, G. (2005) Mathematical modelling of magnetically targeted drug delivery. J. Magn. Magn. Mater. 293, 455-463.

Hernández, R., Sarafian, A., Lopez, D. and Mijangos, C. (2004)

Viscoelastic properties of poly(vinyl alcohol) hydrogels and ferrogels obtained through freezing-thawing cycles. Polymer 45, 5543-5549.

Hernández, R., Zamora-Mora, V., Sibaja-Ballestero, M., Vega-Baudrit, J., López, D. and Mijangos, C. (2009) Influence of iron oxide

nanoparticles on the rheological properties of hybrid chitosan ferrogels. Journal of Colloid and Interface Science 339, 53-59.

Hong, R. Y., Feng, B., Liu, G., Wang, S., Li, H. Z., Ding, J. M., Zheng, Y.

and Wei, D. G. (2009) Preparation and characterization of Fe³O⁴/polystyrene composite particles via inverse emulsion polymerization. J. Alloy. Compd. 476, 612-618.

Huang, H. Y., Hu, S. H., Chian, C. S., Chen, S. Y., Laia, H. Y.and Chen, Y. Y. (2012) Self-assembling PVA-F127 thermosensitive

Hu, S. H., Liu, T. Y., Liu, D. M. and Chen, S. Y. (2007) Controlled pulsatile drug release from a ferrogel by a high-frequency magnetic field. Macromolecules 40, 6786-6788.

Huang, W. C., Hu, S. H., Liu, K. H., Chen, S. Y. and Liu, D. M. (2009) A flexible drug delivery chip for the magnetically-controlled release of anti-epileptic drugs. J. Control. Release 139, 221-228.

Jain, A. K. and Panchagnula, R. (2000) Skeletal drug delivery systems.

Int. J. Pharm. 206, 1-12.

Jain, T. K., Reddy, M. K., Morales, M. A., Leslie-Pelecky, D. L. and Labhasetwar, V. (2008) Biodistribution, clearance, and

biocompatibility of iron oxide magnetic nanoparticles in rats. Mol.

Pharm. 5, 316-327.

Janes, K. A., Fresneau, M. P., Marazuela, A., Fabra, A. and Alonso, M. J.

(2001) Chitosan nanoparticles as delivery systems for doxorubicin. J.

Control. Release 73, 255-267.

Jeanie L. and Drury, D. J., (2003) Hydrogels for tissue engineering:

scaffold design variables and applications. Biomaterials.

24,4337-4351.

Jeong, B., Bae, Y. H. and Kim, S. W. (2000) Drug release from biodegradable injectable thermosensitive hydrogel of

PEG-PLGA-PEG triblock copolymers. J. Control. Release 63, 155-163.

Jeong, B., Choi, Y.K., Bae, Y.H., Zentner, G., Kim , S.W., (1999) New biodegradable polymers for injectable drug delivery systems. Journal of Controlled Release.62 ,109-114.

Jeong, U., Teng, X., Wang, Y., Yang, H. and Xia, Y. (2007) Superparamagnetic colloids: controlled synthesis and niche applications. Adv. Mater. 19, 33-60.

Jun, Y. W., Seo, J. W. and Cheon, J. (2008) Nanoscaling laws of magnetic nanoparticles and their applicabilities in biomedical sciences. Accounts Chem. Res. 41, 179-189.

Kim, S. J., Lee, C. K. and Kim, S. I. (2004) Electrical/pH responsive properties of poly(2-acrylamido-2-methylpropane sulfonic

acid)/hyaluronic acid hydrogels. J. Appl. Polym. Sci. 92, 1731-1736.

Kim, Y. I., Kim, D. and Lee, C. S. (2003) Synthesis and characterization of CoFe²O⁴ magnetic nanoparticles prepared by

T. and Weissleder, R. (2000) Tat peptide-derivatized magnetic

nanoparticles allow in vivo tracking and recovery of progenitor cells.

Nat. Biotech. 18, 410-414.

Li, F., Wang, H., Wang, L. and Wang, J. (2007a) Magnetic properties of ZnFe2O4 nanoparticles produced by a low-temperature solid-state reaction method. J. Magn. Magn. Mater. 309, 295-299.

Li, L. L., Chen, D., Zhang, Y. Q., Deng, Z., Ren, X. L., Meng, X. W., Tang, F. Q., Ren, J. and Zhang, L. (2007b) Magnetic and fluorescent multifunctional chitosan nanoparticles as a smart drug delivery system. Nanotechnology 18, 5102-5017.

Li, X. H., Xu, C. L., Han, X. H., Qiao, L., Wang, T. and Li, F. S. (2010) Synthesis and magnetic properties of nearly monodisperse CoFe²O⁴ nanoparticles through a simple hydrothermal condition. Nanoscale Res. Lett. 5, 1039-1044.

Lin, J. J., Chen, J. S., Huang, S. J., Ko, J. H., Wang, Y. M., Chen, T. L.

and Wang, L. F. (2009) Folic acid-Pluronic F127 magnetic

nanoparticle clusters for combined targeting, diagnosis, and therapy applications. Biomaterials 30, 5114-5124.

Liu, T. Y., Hu, S. H., Liu, D. M., Chen, S. Y. and Chen, I. W. (2009a) Biomedical nanoparticle carriers with combined thermal and magnetic responses. Nano Today 4, 52-65.

Liu, T. Y., Hu, S. H., Liu, K. H., Shaiu, R. S., Liu, D. M. and Chen, S. Y.

(2008) Instantaneous drug delivery of magnetic/thermally sensitive nanospheres by a high-frequency magnetic field. Langmuir 24, 13306-13311.

Liu, T. Y., Liu, K. H., Liu, D. M., Chen, S. Y. and Chen, I. W. (2009b) Temperature-sensitive nanocapsules for controlled drug release caused by magnetically triggered structural disruption. Adv. Funct.

surface modification and applications in chemotherapy. Adv. Drug Deliv. Rev. 63, 24-46.

Munnier, E., Cohen-Jonathan, S., Linassier, C., Douziech-Eyrolles, L., Marchais, H., Souc, M., Herv, K., Dubois, P. and Chourpa, I. (2008) Novel method of doxorubicin-SPION reversible association for magnetic drug targeting. Int. J. Pharm. 363, 170-176.

Muzzarelli, R. A. A. (2009) Genipin-crosslinked chitosan hydrogels as biomedical and pharmaceutical aids. Carbohydr. Polym. 77, 1-9.

Nigam, S., Barick, K. C. and Bahadur, D. (2010) Development of citrate-stabilized Fe3O4 nanoparticles: Conjugation and release of doxorubicin for therapeutic applications. J. Magn. Magn. Mater. 323, 237-243.

Park, J. H., Saravanakumar, G., Kim, K. and Kwon, I. C. (2010) Targeted delivery of low molecular drugs using chitosan and its derivatives.

Adv. Drug Deliv. Rev. 62, 28-41.

Peniche, C., Argüelles-Monal, W., Peniche, H. and Acosta, N. (2003) Chitosan: An attractive biocompatible polymer for

microencapsulation. Macromol. Biosci. 3, 511-520.

Qin, J., Laurent, S., Jo, Y. S., Roch, A., Mikhaylova, M., Bhujwalla, Z.

M., Muller, R. N. and Muhammed, M. (2007) A high-performance magnetic resonance imaging t2 contrast agent. Adv. Mater. 19, 1874-1878.

Qin, R., Li, F., Jiang, W. and Liu, L. (2009) Salt-assisted low temperature solid state synthesis of high surface area CoFe²O⁴ nanoparticles. J.

Mater. Sci. Technol. 25.

Qiu, Y. and Park, K. (2001) Environment-sensitive hydrogels for drug delivery. Adv. Drug Deliv. Rev. 53, 321-339.

Richert, H., Surzhenko, O., Wangemann, S., Heinrich, J. and Görnert, P.

(2005) Development of a magnetic capsule as a drug release system for future applications in the human GI tract. J. Magn. Magn. Mater.

293, 497-500.

Satarkar, N. S. and Hilt, J. Z. (2008) Magnetic hydrogel nanocomposites for remote controlled pulsatile drug release. J. Control. Release 130, 246-251.

Steinfeld, U., Pauli, C., Kaltz, N., Bergemann, C. and Lee, H. H. (2006) T lymphocytes as potential therapeutic drug carrier for cancer treatment.

superparamagnetic nanoparticles for targeted cellular uptake and detection by MRI. J. Biomed. Mater. Res. Part A 78A, 550-557.

Sun, Y., Chen, Z. L., Yang, X. X., Huang, P., Zhou, X. P. and Du, X. X.

(2009) Magnetic chitosan nanoparticles as a drug delivery system for targeting photodynamic therapy. Nanotechnology 20, 1-8.

Varga, Z., Filipcsei, G., Szilagyi, A. and Zrinyi, M. (2005) Electric and magnetic field-structured smart composites. Macromol. Symp. 227, 123-133.

Varga, Z., Filipcsei, G. and Zrinyi, M. (2006) Magnetic field sensitive functional elastomers with tuneable elastic modulus. Polymer 47, 227-233.

Weng, Y. J., Weng, Y. C., Yang, S. Y. and Wong, J. L. (2009) A novel electromagnetism-assisted imprinting technology to replicate

microstructures onto a large-area curved surface using a flexible magnetic mold. Polym. Adv. Technol. 20, 92-97.

Xu, J., Yang, H., Fu, W., Du, K., Sui, Y., Chen, J., Zeng, Y., Li, M. and Zou, G. (2007) Preparation and magnetic properties of magnetite nanoparticles by sol-gel method. J. Magn. Magn. Mater. 309, 307-311.

Xulu, P. M., Filipcsei, G. and Zrínyi, M. (2000) Preparation and responsive properties of magnetically soft

poly(N-isopropylacrylamide) gels. Macromolecules 33, 1716-1719.

Yapar, E., Kayahan, S. K., Bozkurt, A. and Toppare, L. (2009)

Immobilizing cholesterol oxidase in chitosan-alginic acid network.

Carbohydr. Polym. 76, 430-436.

Yong Qiu, Kinam Park, (2001) Environment-sensitive hydrogels for drug delivery. Advanced Drug Delivery Reviews. 53,321-339.

Zhang, J. L., Srivastava, R. S. and Misra, R. D. K. (2007) Core/shell

Colloid Polym. Sci. 278, 98-103.

Zrínyi, M., Barsi, L. and Buki, A. (1997) Ferrogel: a new

magneto-controlled elastic medium. Polym. Gels Networks 5, 415-427.

Zrínyi, M., Szabo, D. and Kilian, H. G. (1998) Kinetics of the shape change of magnetic field sensitive polymer gels. Polym. Gels Networks 6, 441-454.