以推剪法及旋風處理法收獲之草蛉卵粒，
如圖3。推剪法與次氯酸鈉法兩種處理結果比 較 (表 1)：推剪試驗中以 1 mm 高度之刀頭組 別結果最優良，而次氯酸鈉處理組別中以1.5%
濃 度 搭 配60 s 處理時間之效果最優。推剪法 及 次 氯 酸 鈉 法 處 理 結 果， 最 優 組 別 比 較， 卵 收穫率分別為90.2 ± 0.6 vs. 80.0 ± 2.3%，兩
組無顯著差異 (Levene’s test: P = 0.048; Welch’s ANOVA: F6, 8.00 = 155.43, P < 0.001; Dunnett’s T3 test: P = 0.135)，孵化率為 80.8% ± 2.8% vs.
70.0% ± 3.6%， 兩 組 間 無 顯 著 差 異 (Levene’s test: P = 0.119; ANOVA: F6, 21.00 = 110.574, P <
0.001; Fisher’s LSD test: P = 0.142)，成蟲獲得率 為54.2% ± 3.4% vs. 46.7% ± 4.1%，兩組間無顯 著差異 (Levene’s test: P = 0.026; Welch’s ANO-VA: F6, 8.00 = 849.23, P < 0.001; Dunnett’s T3 test:
P = 0.251)。 對 照 組 (剪 刀 剪 取 法) 之 卵 收 穫 率、孵化率及成蟲獲得率，則分別為 100.0%
± 0.0%、90.8% ± 1.6% 及 60.8% ± 0.8%。若以 3 種處理組之卵收穫率最優組別：1 mm 推剪 法、60 s 次氯酸鈉法及剪刀剪取法相比較操作 之時間效率，操作時間分別為1 s、60 s 及 2–3 min，以推剪法搭配旋風處理法為最佳，次氯 酸鈉法次之，而剪刀剪取之對照組最耗費收穫 時間。剪刀法雖具最佳的卵收穫率、孵化率及 成蟲獲得率，但所耗費之時間成本巨大，不適 合應用於實務操作。推剪法搭配旋風離心法收 獲草蛉卵粒，相較於次氯酸鈉法有操作時間及 空間應用之優勢，且有較佳的卵收穫率、孵化 率及成蟲獲得率。
1 mm 1 mm
圖3. 推剪搭配旋風分離法收穫的基徵草蛉卵。
Fig. 3. Eggs of Mallada basalis harvested using a hair clipper with cyclonic separation.
259 以電動推剪收獲基徵草蛉卵
草 蛉 卵 柄 易 受 重 力 彎 曲， 使 卵 粒 緊 靠 底 面， 本 文 設 計 之 推 剪 法 先 以 負 壓 氣 流 拉 伸 卵 柄，使卵粒遠離刀刃，避免受上部刀刃撞擊，
並 將 剪 下 的 卵 粒 立 即 移 除， 以 避 免 受 二 次 剪 取 造 成 損 傷。 提 前 抽 風 可 除 去 表 面 髒 污， 配 合 以 鐵 氟 龍 塗 布 之 刀 刃 表 面， 避 免 灰 塵 及 成 蟲 的 黏 性 排 遺 沾 黏 於 刀 刃， 造 成 卵 柄 黏 附。
本 文 採 用 旋 風 離 心 法 (Hsiu et al. 2019) 來收 穫 草 蛉 卵 粒， 以 避 免 濾 網 式 吸 塵 易 使 大 量 卵 粒 於 濾 網 處 堵 塞 且 擠 壓 受 損， 且 濾 網 處 的 高 速 氣 流 易 使 卵 柄 產 生 靜 電， 導 致 卵 粒 彼 此 沾 黏形成叢集 (clump) 而不易分離 (Nordlund &
Correa 1995a)。本文推剪法步驟較以往的化
學 溶 解 及 電 熱 熔 融 (melted) 法簡易，且操作 時間極短、安全性高，並且無化學浸泡法所需 之液體空間，無尼龍球或刮鬍刀法造成的卵柄 糾結現象，也無電熱法之高耗電帶來的安全問 題 (Nordlund & Correa 1995a, 1995b; Bezerra et al. 2014)，可有效率的收獲草蛉卵粒並縮小 占用空間，有機會發展為小體積之自動收穫系 統。
誌謝
本試驗承行政院農業委員會農業試所應用 動物組益蟲研究室助理張敏專小姐及王俞良小 姐協助執行，謹此致謝。
表 1. 推剪法搭配次氯酸鈉溶解法取下基徵草蛉卵粒之成功率、孵化率及成蟲獲得率。
Table 1. Egg harvest (%), hatch (%), and egg-adult (%) for removing the egg stalk of Mallada basalis using the clipper and sodium hypochlorite method.
Method of removing egg stalk Eggs harvest %
(Mean ± SEMz) Hatch %
(Mean ± SEM) Egg-adult % (Mean ± SEM)
Scissors 100.0 ± 0.0 ay 90.8 ± 1.6 a 60.8 ± 0.8 a
Clipper with cyclonic separation
1 mm 90.2 ± 0.6 ab 80.8 ± 2.8 ab 54.2 ± 3.4 ab
2 mm 63.8 ± 4.8 c 70.8 ± 1.6 b 47.5 ± 1.6 b
3 mm 45.8 ± 1.8 d 54.2 ± 4.4 c 35.8 ± 1.6 cd
Sodium hypochlorite solution
1.5%, 60 s 80.0 ± 2.3 b 70.0 ± 3.6 b 46.7 ± 4.1 bc
3.0%, 30 s 80.1 ± 4.7 b 51.7 ± 3.2 c 33.3 ± 3.0 d
6.0%, 10 s 78.1 ± 5.1 bc 0.3 ± 0.0 d 0.0 ± 0.0 e
z SEM: standard error of the mean.
y Mean ± SEM within the same column followed by the same letters are not significantly different at the 5% level by least significant difference (LSD) test.
附錄 以不同濃度及處理時間之次氯酸鈉溶解法取下基徵草蛉卵粒之成功率及孵化率。
Appendix Egg harvest (%) and hatch (%) for removing the egg stalk of Mallada basalis using the sodium hypo-chlorite method with different concentrations and treatment durations.
Sodium hypochlorite concentration (%) Treatment duration
(s) Eggs harvest %
(Mean ± SEMz) Hatch % (Mean ± SEM)
1.5 10 18.0 ± 2.5 dey 84.9 ± 7.9 a
30 51.3 ± 3.1 d 73.4 ± 5.4 a
60 73.2 ± 4.5 bc 76.4 ± 3.2 a
3.0 10 46.0 ± 3.8 d 48.5 ± 4.8 b
30 79.9 ± 3.6 abc 44.8 ± 6.1 bc
60 86.5 ± 2.6 ab 26.0 ± 4.2 cd
6.0 10 69.8 ± 4.3 c 7.1 ± 2.4 de
30 89.5 ± 2.0 a 3.5 ± 1.5 e
60 92.5 ± 2.9 a 3.4 ± 1.5 e
z SEM: standard error of the mean.
y Mean ± SEM within the same column followed by the same letters are not significantly different at the 5% levels by least signifi-cant difference (LSD) test.
261 以電動推剪收獲基徵草蛉卵
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