結論 - 於逆流層析中以具有親和性反微胞萃取蛋白質溶菌酶

本實驗中，藉由CB-Span 85 結合逆流層析儀來萃取蛋白質混合物。在樣品中加入卵白蛋白與溶菌酶混合液來進行萃取，發現雖然在第 5 管時，溶菌酶會受到其它蛋白質靜電作用的影響而出現，在第 11管收集液中，仍然只有溶菌酶的存在，由於卵白蛋白不與 CB染料分子作用，也就是對靜相沒有任何滯留力，在第 11 管的收集液，才只有溶菌酶的存在。接下來使用溶菌酶與牛血清蛋白混和物當樣品，

CB 染料分子對其二者皆有親和力的影響，利用界面活性劑所形成水核中心的大小來調控蛋白質進出反微胞系統，使溶菌酶被萃取，而牛血清蛋白不被萃取進入反微胞系統中。拿溶菌酶、卵白蛋白與牛血清蛋白之混合物來進行萃取，雖然溶菌酶會受到其他蛋白質的靜電作用影響，使其回收率下降，但在CB-Span 85 所形成的反微胞系統下，

其反萃時收集液中只有溶菌酶的存在，這說明了CB-Span 85 只對溶菌酶這種蛋白質有專一性的親和作用力。

在本實驗中，使用CB-Span 85結合逆流層析儀來萃取蛋白質混合物這項技術，不僅製備容易而且價格低廉，是種應用性非常高的萃取系統，接下來我們還可以使用其他種類之界面活性劑或加入其他的萃取劑，來提升萃取效率，以及放大萃取容量，使得這項技術更具實用性。我們預期具親和性的反微胞系統可以成為分離蛋白質的技術。

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在文檔中於逆流層析中以具有親和性反微胞萃取蛋白質溶菌酶 (頁 61-66)