Appendix Fig.1 generation of muscle-specific NRIP knockout (cKO) mice.
Schematic illustration of genomic structure of wild-type (WT) NRIP, loxP-floxed NRIP, and NRIP-deleted alleles (Chen et al., 2015). The loxP-floxed allele contains a neo cassette with two flanked loxP sequences in intron 1 and a loxP sequence in intron 2.
NRIP exon 2 would be deleted by Cre recombinase driven by MCK promoter.
Appendix Fig.2 Primer sequences.
Appendix Fig.3 Plasmid backbones list.
Primers for Cre recombinase genotyping
Primer name Primer Sequence (5’ to 3’)
Cell staining
Antibody name company Cat No. Dilution
Wheat germ agglutinin (WGA)-555 conjugate
ThermoFisher W32464 1:200
Calnexin abcam ab22595 1:200
Flag Sigma F3165 1:200
GFP Santa Cruz sc-9996 1:50
Western blot
Antibody name company Cat No. Dilution
EGFP abcam ab183734 1:10000
Flag Sigma F3165 1:10000
GAPDH AbFrontier LF-PA0212 1:10000
Tissue staining
Antibody name company Cat No. Dilution
EGFP abcam ab183734 1:200
Alexa-594-conjugated α-bungarotoxin
Life techologies B13423 1:1000
Neurofilament abcam ab8135 1:500
synaptophysin abcam Ab32127 1:250
NeuN Millipore MABN140 1:500
ChAT Millipore AB144P 1:250
Appendix Fig.4 Antibody lists.
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