Chapter 5 Conclusion
Using the XYZ image stacks acquired by in vivo harmonic generation biopsy system,
14 parameters were defined to inspect morphological changes of viable epidermis and
mainly dermal papillae in a three-dimensional point of view. Among these parameters, 10
parameters were found to be related to age. Only 1 parameter was found related to gender.
From the analysis of whole set of these parameters combined, the deduction of changes of
skin was made to describe the overall effect of intrinsic aging (Fig. 24 and 25).
The results of the in vivo analysis of viable epidermis thickness implied the
flattened dermal-epidermal junction in the aged skin. The results of the in vivo analysis
of isolated dermal papilla indicated that the intrinsic aging resulted in the shrinkage of
the isolated dermal papilla in size, which might reduce the interface area between dermis
and epidermis, and the isolated dermal papilla did not extend its width with age.
Moreover, the analysis also showed that both large and small isolated dermal papillae
existed in the younger skin, but there were only small isolated dermal papillae remained
in the elder skin (Fig. 24). The results of the in vivo analysis of dermal papillae within
dermal papilla zone indicated the dermal papillae were not fewer in the aged skin.
Furthermore, dermal papilla zone became thinner with increasing age, but the dermal
ϲϬ
papillae within dermal papilla zone were not reduced in volume. The independent dermal
papillae connected to each other quicker with age, and the whole dermal papillae within
dermal papilla zone extended in width (Fig. 25). Moreover, the analysis of the 3D
interdigitation index showed the decreasing interface area of the dermal-epidermal
junction with age, which might related to the fragility of the aged skin due to the
weakening of epidermal-dermal adherence.
ϲϭ
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