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In this study, we found that eGFR was moderately correlated with cognitive

function, cortical thickness, and GM volume. Study participants with reduced kidney

function, had impaired cognitive performance, less cortical thickness, and smaller

hippocampal volume and GM volume than did subjects with normal renal function. In

further regression analysis, we found that renal function and age were major

contributing factors for cortical thickness, GM and hippocampal volume after

controlling for confounding factors.

A few studies have reported brain atrophy and WMH burden in patients with

CKD or renal failure (9, 10, 28-31). A meta-analysis of numerous structural and

functional neuroimaging studies that examined children and adults with CKD

identified several clear trends, including cerebral atrophy, WMH, cerebral infarction,

microbleeding, and cerebral blood flow pattern with affective disorders (31). In the

retrospective population-based Rotterdam Scan Study, subjects with low eGFR had

smaller brain and deep WM volumes and more WMH (30). No close relationship was

found between eGFR and GM or WM volume, which is not fully consistent with our

results (30). The demographic characteristics of participants in the Rotterdam Scan

Study differed from those in the present study subjects, such as lower percentage of

diabetes and hypertension, and the higher ratio of male patients to female patients

than those in the present population. Nevertheless, the Rotterdam Scan Study lacked

neuropsychological assessment, which precluded correlation of brain measurements

with cognitive performance. Most previous imaging analyses were focus on grading

of WMH and detection of ischemic infarction, which were based on qualitative visual

rating, rather than objective quantitative measurement. However, little about

quantitative measurement of brain structure in patients with CKD was reported.

Furthermore, the present study quantitatively showed the GM, WM and hippocampus

volumes decreased by 6.2%, 8.9% and 10.7%, respectively, in patients with CKD

compared with control subjects. The hippocampal volume seems to be more affected

by eGFR and age than global GM and WM volume.

In present study, significant cortical thinning in bilateral superior temporal sulci

and medial lingual gyri was found in these patients with CKD and they showed

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lower global cognition scores and poorer performance in working memory (forward

digit span) than those in the control group. In patients with mild cognitive impairment

or dementia, cortical thinning has been found in association with cardiovascular risk

factors, such as hypertension and diabetes (32). However, eGFR and age were

independently related to GM volume and cortical thickness in analyses controlling for

the vascular component in the present study. The CKD group also had a significant

reduction in hippocampal volume. Hippocampal atrophy has been shown to be an

effective marker differentiating people with normal and impaired cognition,

regardless of WMH and lacunar infarcts (33). Thus, renal function seems to be an

independent factor affecting cortical atrophy and thinning via an uncertain mechanism

rather than cerebrovascular risk factors. Nevertheless, the difference in WMH

between patients with CKD and control subjects didn’t reach significant level in this

study. This result may be explained by the more frequent occurrence of WMH in

patients with advanced CKD and those receiving hemodialysis, whereas about half of

patients in our study had moderate CKD.

In line with previous studies (2, 5, 34), the present study also showed that

patients with CKD had poorer global cognitive function, executive function, verbal

and working memory than the control subjects. A recent meta-analysis found that

both cross-sectional and longitudinal studies have demonstrated a significantly

increased risk of cognitive impairment in patients with CKD (35). Furthermore,

another longitudinal cohort study conducted in Japan found that CKD was associated

with the risk of dementia in patients with v independent of vascular components (36).

These results are consistent with the hypothesis of a connection between renal

impairment and dementia risk.

The close relationship between cardiovascular risk factors and dementia with

small vessel disease is well established (37). Recently, CKD was found to be related

to neurological disorders and this brain–renal connection is thought to involve small

vessel disease in kidney and brain, based on hemodynamic similarities (38). The main

pathologic vascular feature of CKD and albuminuria, implied impaired kidney

vascular integrity, which might also be found in other organs with similar vascular

bed. Recent study showed lower eGFR was independently associated with lower

cerebral blood flow. The impaired cerebral autoregulation, probably leading to

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hypoperfusion, was thought to increased risk of dementia (39). Furthermore it has

been suggested that various toxins have been involved in the pathogenesis of

cognitive impairment in uremic patients(40). Oxidative radicals, elevated serum

homocysteine level and inflammation have also been related to cognitive impairment

in dialysis patients. These toxic substance could lead to vascular endothelial

dysfunction and thus aggravate atherosclerosis and increase risk of dementia(41).

These studies implied that impaired renal function, as measured by decreased eGFR,

with alterations in water and electrolytes balance, accumulation of vasoactive species,

and chronic inflammation is related to cerebral small vessel disease, independent of

cardiovascular risk factors (30). Such findings are consistent with our study results:

impaired eGFR is correlated with reduced GM volume and global cognitive

performance, independent of other cardiovascular disease. In contrast, some study

showed that mild CKD was associated with an increased risk of Alzheimer disease

(AD) (36). The longitudinal BRain IN Kidney disease (BRINK) study found smaller

GM volume in region of interests (ROIs) associated with both AD (tempo-parietal

areas) and vascular cognitive impairment (VCI )(frontal lobes), worse cognition

function than patients without CKD(42, 43). Neuropathology Group of the Medical

Research Council Cognitive Function and Ageing Study (MRC CFAS) reported both

Alzheimer type and vascular pathology were found in most elder sample with

cognitive declineby necropsy(44). Thus the possibilities of mixed AD and VCI in

CKD group should be also taken into account.

The strengths of this study include the examination of patients with moderate

CKD and the use of automated MRI analysis, which make it possible to accurately

quantify cortical thinning and GM and WM atrophy and to investigate subcortical

WM lesions. The associations between CKD and cognitive performance, cortical GM

volume, cortical thickness, as well as eGFR were clearly demonstrated. To my

knowledge, this study is the first study on cognitive impairment in patients with CKD

based on comprehensive quantitative measurement of brain structure and renal

function.

This study has some limitations. First, diabetes was more prevalent among

patients with CKD than among the control subjects, reflecting a similar difference in

the Taiwanese population (the prevalence of diabetes is about 15% in individuals with

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and 4% in those without CKD) (45). To eliminate the effect of this difference,

analysis with multiple linear regression model adjusted for age, education, diabetes,

and other comorbidities was applied. Second, patients in the present study were

recruited from a tertiary medical center, rather than from a community-based hospital,

which may have led to selection bias. Third, smoking is indeed a major cardiovascular

factor and it was not controlled in the present study. This might cause some bias.

Forth, the present study did not perform CSF markers check-up and PET isotope study,

the possibilities of prodrome of AD in CKD group cannot be excluded. Finally,

though cortical thickness, GM volume, and MMSE score were lower in the CKD

group than in the control subjects, the cause–effect relationships between renal

function, brain morphometric features and cognitive performance remain uncertain

because of the cross-sectional study design and lack of longitudinal follow-up.

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