Figure 1 I/O and PPF results recorded in the contralateral Dm
(A) Brain slice was placed over the electrodes on the MED probe and a stimulating cathode (yellow square) and a recording cathode (red square)
20 50 100 150 200
were chosen. (B) Divisions of dorsal telencephalon slice of zebrafish. (C) The input-output relationship of Dl-Dm pathway of contralateral side. (D) Pair-pulse facilitation of Dl-Dm pathway of contralateral side. (E) Single trace of the components of contralateral Dm field potential. (F) Single trace of the components of ipsilateral Dm field potential.
Figure 2
Dl-evoked long-term potentiation (LTP) in the ipsilateral and contralateral side of Dm. (A) LTP of the contralateral side of Dm (group data). (B) LTP of the ipsilateral side of Dm (group data). High frequency stimulation (100 Hz) was applied (arrow) in the Dl division.
The amplitude of population spike of both sides of LTP was 1.5 times larger than the amplitude of baseline and can last at least 1 hour. Each point represents the mean ± SEM of the population spike amplitude. n=16
-10 0 10 20 30 40 50 60 70 PS amplitude (% of baseline)
B LTP-ipsilateral PS amplitude (% of baseline)
LTP-contralateral
Figure 3
NMDA receptor antagonist, D-AP5, block the formation of LTP in Dl-Dm pathway of contralateral and ipsilateral side. (A) D-AP5 on LTP induction in the contralateral side of Dm induction (group data). (B) D-AP5 on LTP induction in the ipsilateral side of Dm induction (group data). 30 µM of D-AP5 (solid line) was applied after 10 mins of baseline recording. The first HFS (arrow) was delivered 15 mins after the start of drug perfusion. The second HFS (arrow) was delivered 30 mins after the washout of D-AP5. LTP was not able to induct under the effect of D-AP5, but the second HFS was able to induce LTP after the washout of D-AP5.
Each point represents the mean ± SEM of the population spike amplitude.
n=9 PS amplitude (% of baseline)
AP5-ipsilateral PS amplitude (% of baseline)
AP5-contralateral
Figure 4
Differences of LTP amplitude in PS between stimulation given from left or right hemisphere of Dl in contralateral and ipsilateral side. (A) Early phase of LTP (5-15 mins after HFS), the amplitude of population spike of contralateral side of Dm, following HFS given from the left hemisphere of Dl showed the trend of stronger than stimulation given from the right hemisphere. (B) The early phase (5-15 mins after HFS), the middle phase (25-35 mins after HFS) and the late phase (45-55 mins after HFS) of LTP, the amplitude of population spike of ipsilateral side of Dm, following HFS given from the left hemisphere of Dl was stronger than stimulation given from the right hemisphere. Each point represents the mean ± SEM of the population spike amplitude.
-10 0 10 20 30 40 50 60 70 PS amplitude (% of baseline)
L R
ipsilateral side. (B) Following stimulation given from right hemisphere of Dl, the early phase, middle phase, late phase and 60 mins after HFS, the amplitude of population spike of contralateral side of Dm were stronger than the one of ipsilateral side. Each point represents the mean ± SEM of the population spike amplitude. PS amplitude (% of baseline)
contralateral ipsilateral
A Stimulation on the left side
-10 0 10 20 30 40 50 60 70 PS amplitude (% of baseline)
contralateral
Figure 6
LFS-evoked long-term depression (LTD) in the ipsilateral and contralateral side of Dm. (A) LTD of the contralateral side of Dm. (B) LTD of the ipsilateral side of Dm. Low frequency stimulation (1 Hz) was applied (arrow) in the Dl division. The amplitude of population spike of both sides of LTP was 80 % smaller than the amplitude of baseline and can last at least 1 hour. Each point represents the mean ± SEM of the PS amplitude (% of baseline)
LTD-ipsilateral PS amplitude (% of baseline)
LTD-contralateral
Figure 7
Long-term depression induced by mGluR-1 agonist, DHPG induced LTD in the ipsilateral and contralateral side of Dm. (A) DHPG on LTD induction in the contralateral side of Dm induction. (B) DHPG on LTD induction in the ipsilateral side of Dm induction. (C) The comparison on PS amplitude of DHPG-induced LTD in contralateral and ipsilateral Dm. 40 µM of DHPG was applied for 10 mins after 10 mins of baseline recording. The amplitude of population spike of both sides of PS amplitude (% of baseline)
DHPG-contralateral PS amplitude (% of baseline)
DHPG-ipsilateral fEPSP amplitude (% of baseline)
DHPG
contralateral ipsilateral C
*
LTD was 70 % smaller than the amplitude of baseline and can last at least 1 hour. Each point represents the mean ± SEM of the population spike amplitude. n=9
Figure 8
The input-output (IO) curve of Dl-Dm pathway in the ipsilateral and contralateral side of Dm. (A) IO curve of contralateral side of Dm. (B) IO curve of ipsilateral side of Dm. The amplitude of population spike gradually increased from threshold following the increasing stimulation intensity until smoothly saturated in both ipsilateral and contralateral side of Dm. n=14
Figure 9
Comparison of input-output (IO) curve between stimulation given from left or right hemisphere of Dl in contralateral and ipsilateral side. (A) Comparison of contralateral side of Dl-Dm pathway following stimulation given from left and right side (B) Comparison of ipsilateral
Figure 10
Comparison of input-output (IO) curve of contralateral and ipsilateral side of Dm at Dl-Dm pathway. (A) IO comparison of contralateral side and ipsilateral side of Dl-Dm pathway following stimulation given from left side (B) IO comparison of contralateral side and ipsilateral side of Dl-Dm pathway following stimulation given from right side. Each point represents the mean ± SEM of the population spike amplitude. n=7 A Stimulation on the left side
0 10 20 30 40 50 B Stimulation on the right side
Figure 11
The paired pulse ratio (PPF) of Dl-Dm pathway in the ipsilateral and contralateral side of Dm. (A) PPF of contralateral side of Dm. (B) PPF of ipsilateral side of Dm. (C) & (D) Paired pulse facilitation trace of Dm division of contralateral and ipsilateral side, respectively. Population spike in the Dm division were collected using varying interpulse intervals
20ms
(20, 50, 100, 150, 200 ms). Pair pulse ratio refers to the second field potential area divide the first field potential area. n=12
Figure 12
Differences of paired pulse ratio (PPF) between stimulation given from left or right hemisphere of Dl in contralateral and ipsilateral side. (A) PPF comparison of contralateral side of Dl-Dm pathway following stimulation given from left and right side (B) PPF comparison of ipsilateral side of Dl-Dm pathway following stimulation given from
Figure 13
Differences of paired pulse ratio (PPF) between ipsilateral and contralateral side of Dm following stimulation given from left and right hemisphere of Dl. (A) PPF comparison of contralateral side and ipsilateral side of Dl-Dm pathway following stimulation given from left side (B) PPF comparison of contralateral side and ipsilateral side of Dl-Dm pathway following stimulation given from right side. Each point represents the mean ± SEM of the population spike amplitude. n=12
20 50 100 150 200 B Stimulation on the right side
Figure 14
NMDA receptor distribution of left and right hemisphere of telencephalon. In NMDAR1a, the mRNA expression of left hemisphere showed the tendency of higher expression than right hemisphere, whereas NMDAR1b, no difference or tendency was observed. n=4
NMDAR1a-L NMDAR1a-R NMDAR1b-L NMDAR1b-R 0
1 2 3 4
group
NMDAR relative mRNA expression
NMDAR1a-L NMDAR1a-R NMDAR1b-L NMDAR1b-R