折射率檢測器 RI750F
Acme 9000, YOUNG LIN INSTRUMENT, Korea
11 Acme 6000 GC Acme 6000, YOUNG LIN INSTRUMENT, Korea
Table 7、氣相色層分析儀之操作條件
GC 型號 China Chromatography 8700 層析管規格
J&W Scientific, DB-5 Fused Silica Capillary Column, 30m, 0.53m,
5µm film thickness 偵測器 Flame ionized detector (FID)
載流氣體 N2
OVEN控制溫度 110 ℃
注入口控制溫度 210 ℃
偵測器控制溫度 230 ℃
載流氣體控制壓力 0.8 bar
氫氣氣體控制壓力 0.5 bar
空氣氣體控制壓力 0.5 bar
樣品注入量 Liquid sample：0.3 µl Gas sample：0.5 mL
Table 8、高效能液相層析儀之操作條件 HPLC 型號 YOUNGLIN INSTRUMENT
Acme 9000 備註
層析管規格 Hypersil ODS (5µL, 250×4.6 mm)
移動相1 0.1mol / L 丙酮(acetone)
UV& 移 動 相 1 僅 可 測 DMSO
(DMSO2訊號太弱) 移動相2 0.025% / L 硫酸(sulfuric acid) RI& 移 動 相 2 可 測 得
DMSO、DMSO2 流速 1.0 mL/min
UV detector
檢測波長 225nm
Table 9、不同菌種降解 DMSO 之比較實驗組別
組別 1 組別 2 組別 3 組別 4
A 菌種
含 50 mg/L sucrose
A 菌種 無 sucrose
B 菌種
含 50 mg/L sucrose
B 菌種 無 sucrose 註：經過馴養表示為 A 菌種，未經過馴養表示為 B 菌種。
Table 10、緩衝溶液配製表[Dawson et al., 1968]
pH 緩衝溶液種類 所需含量(mL) 配製方法 0.1 M citric acid 238.35
3
0.2 M Na2HPO4 61.65 0.1 M citric acid 145.50 5
0.2 M Na2HPO4 154.50 0.1 M citric acid 52.95 7
0.2 M Na2HPO4 247.05
兩者混合即可，總體積為 300mL。
0.1 M KCl + H3BO3 150 8.5
0.1 M NaOH 30.3 0.1 M KCl + H3BO3 150 10
0.1 M NaOH 131.1
混 合後 以 蒸 餾 水 稀 釋 至 300 mL。
Table 11、不同碳源含量對分解能力之影響實驗組別 蔗糖
含量 (mg/L)
組別 1 控制組 (無菌)
組別 2 (30g 菌)
組別 3 (30g 菌)
組別 4 (30g 菌)
組別 5 (30g 菌)
組別 6 (30g 菌) 低濃度 50 mg 0 mg 50 mg 100 mg 200 mg 300 mg 高濃度 50 mg 0 mg 50 mg 500 mg 1000 mg 1500 mg
Fig. 1、研究架構
利用固定化活性污泥於氣舉式反應器去除光電業廢水中的二甲基亞碸 利用固定化活性污泥於氣舉式反應器去除光電業廢水中的二甲基亞碸 利用固定化活性污泥於氣舉式反應器去除光電業廢水中的二甲基亞碸 利用固定化活性污泥於氣舉式反應器去除光電業廢水中的二甲基亞碸
不同環境因子對活性污泥分解DMSO影響 反應器重覆批次實驗 不同菌種比較搖瓶實驗
懸浮活性汙泥 重覆批次實驗
固定化活性汙泥 重覆批次實驗
懸浮活性汙泥
A 菌 種 + C
A 菌 種 沒
C
B 菌 種 沒 C B
菌 種 + C
懸浮活性污泥 (先前已做)
固定化顆粒
不同 pH
不同 DMSO
固定化顆粒製備
以最佳化條件進行 固定化活性汙泥重覆批次實驗
不同 Sucrose
不同 接菌量
搖瓶重覆批次試驗
分析項目 分析項目分析項目 分析項目：：：：
DMSO濃度、SO42-濃度
pH值、DO、OD、ORP、導電度 NH4+、NO2
-、NO3
-、PO4
3-Biomass(MLSS)
Fig. 2、平面顯示器 ARRAY 製程流程圖
[工業研究院執行經濟部工業局九十三年度專案計畫-平面顯示器產 業環境建構計畫, 2004]
Fig. 3、TFT-LCD 製造程序及廢棄產生源示意圖
[工業研究院執行經濟部工業局九十三年度專案計畫-平面顯示器產 業環境建構計畫, 2004]
Fig. 4、不同菌種降解 DMSO2 之途徑 [Kuniki Kino et al., 2004]
(A)為Hyphomicrobium sp. WU-OM3降解DMSO2的途徑；
(B)為Arthrobacter methylotrophus TGA和Hyphomicrobium
sulfonivorans S1降解DMSO2的途徑。文字縮寫為：DMSO2, dimethyl sulfone; MSA, methanesulfonate; DMSO, dimethyl sulfoxide; DMS, dimethyl sulfide.
Fig. 5、不同 DMSO 處理方法的反應途徑圖 [Koito et al, 1998]
Fig. 6、Set-up of air-lift system:
(1) diaphragm air pump; (2) rotameter; (3) DMSO reservoir;
(4) feeding port; (5) liquid sampling port; (6) settler; (7) airlift;
(8) gas sampling port for DMS using Tedlar bags.
0 20 40 60 80 100 0
25 50 75 100 125 150 175 6.00200 6.25 6.50 6.75 7.00 7.25 7.50
DMSO concentration (mg/L)
TIME (hr)
pH
Fig. 7、Effect of acclimation and addition of sucrose by DMSO or other on dimethyl sulfoxide degradation.
conditions: Temp.: 30oC; the rotational speed: 120 rpm;
initial pH: 7.0; sucrose: 50 mg/L (—■—) A operated with addition of 50 mg/l of sucrose;
(—
●
—) A operated without addition of 50 mg/l of sucrose;(—▲—) B operated with addition of 50 mg/l of sucrose;
(—▼—) B operated without addition of 50 mg/l of sucrose
ٛ
0 10 20 30 40 50 60 70 80 0
20 40 60 80 1002 4 6 8 0.010 2.5 5.0 7.5
TIME(hr) DMSO concentration (mg/L)pHDO (mg/L)
Fig. 8、Effect of pH value on DMSO degradation using the immobilized cells of activated sludge.
Operational conditions: Temp.:30oC;
the rotational speed: 120 rpm Various initial pH value:
(—■—)pH3.0; (—
●
—)pH5.0; (—▲—)pH7.0;(—▽—)pH8.5; (—◇—)pH10.0
0 10 20 30 40 50 60 70 80 0
20 40 60 80 100 2 4 6 8 100 2 4 6 8 10
DMSO concentration (mg/L)
TIME (hr) pHDO (mg/L)
Fig. 9、Effect of pH value on DMSO degradation using the immobilized cells of activated sludge.
Operational conditions: Temp.:30oC;
the rotational speed:120 rpm Various initial pH value:
(—■—)pH3.0; (—
●
—)pH5.0; (—▲—)pH7.0;(—▽—)pH8.5; (—◇—)pH10.0
0 10 20 30 40 50 0
20 40 60 80 100 5.5 6.0 6.5 7.0 7.5 0 2 4 6 8 180 195 210 225 240
DMSO conc. (mg/L)
TIME(hr) pHDO (mg/L)ORP (mv)
Fig. 10、Effect of initial sucrose concentration on DMSO degradation using the immobilized cells of activated sludge.
Operational conditions: Temp.:30oC;
the rotational speed: 120 rpm; initial pH: 7.0;
initial sucrose concentration:
(—□—)control: 50mg/L; (—
●
—)0mg/L;(—▲—)50mg/L; (—▽—)100mg/L;
(—◇—)200mg/L; (—
○
—)300mg/L0 10 20 30 40 0
20 40 60 80 1006.0 6.5 7.0 7.5 4 5 6 7 8 210 220 230 240 250
DMSO conc. (mg/L)
TIME (hr)
pHDO (mg/L)ORP (mv)
Fig. 11、Effect of initial sucrose concentration on DMSO degradation using the immobilized cells of activated sludge.
Operational conditions: Temp.:30oC;
the rotational speed: 120 rpm; initial pH: 7.0;
initial sucrose concentration:
(—■—)control: 50mg/L; (—
●
—)0mg/L;(—▲—)50mg/L; (—▼—)100mg/L;
(—◆—)200mg/L; (—
◄
—)300mg/L0 2 4 6 8 10 12 0
20 40 60 80 100 150 225 300 375 6.0 6.5 7.0 7.5 3.0 4.5 6.0 7.5 180 200 220
DMSO conc. (mg/L)
TIME (hr) Sulfate ion conc. (mg/L)pHDO (mg/L)ORP (mv)
Fig. 12、Effect of initial sucrose concentration on DMSO degradation using the immobilized cells of activated sludge.
Operational conditions: Temp.:30oC;
the rotational speed: 120 rpm; initial pH: 7.0; initial
sucrose concentration:
(—■—)control: 50mg/L; (—
●
—)0mg/L;(—▲—)50mg/L; (—▼—)100mg/L;
(—◆—)200mg/L; (—
◄
—)300mg/L0 5 10 15 20 25 30 35 0
20 40 60 80 100 0 100 200 300 4006.0 6.5 7.0 7.50 2 4 6 1258 150 175 200 225
TIME(hr) DMSO conc. (mg/L)Sulfate conc. (mg/L)pHDO (mg/L)ORP (mv)
Fig. 13、Effect of initial sucrose concentration on DMSO degradation using the immobilized cells of activated sludge.
Operational conditions: Temp.: 30oC;
the rotational speed: 120 rpm; initial pH: 7.0;
initial sucrose concentration:
(—■—)control: 50mg/L; (—●●●●—)0mg/L;
(—▲—)50mg/L; (—▼—)500mg/L;
(—◆—)1000mg/L; (—
◄
—)1500mg/L0 5 10 15 20 0
20 40 60 80 100 120 0 50 100 150 200 250 300 350 6.0 6.5 7.0 7.50 2 4 6 1258 150 175 200
DMSO conc. (mg/L)
TIME(hr) Sulfate conc. (mg/L)pHDO (mg/L)ORP (mv)
Fig. 14、Effect of biomass on DMSO degradation using the immobilized cells of activated sludge.
Operational conditions: Temp.:30oC;
the rotational speed: 120 rpm; initial pH : 7.0;
biomass:
(—■—)0g; (—●●●●—)15g; (—▲—)30g;
(—▼—)45g; (—◆—)60g
0 5 10 15 20 0
20 40 60 80 100 0 100 200 300 400 5.5 6.0 6.5 7.0 7.50 2 4 6 8 140 160 180 200 220 240
DMSO conc. (mg/L)
TIME(hr) Sulfate conc. (mg/L)pHDO (mg/L)ORP (mv)
Fig. 15、Effect of biomass on DMSO degradation using the immobilized cells of activated sludge.
Operational conditions: Temp.:30oC; the rotational
speed: 120 rpm; initial pH : 7.0; biomass:
(—■—)0g; (—●●●●—)15g; (—▲—)30g;
(—▼—)45g; (—◆—)60g
0 100 200 300 400 500
0 2 4 6 8 10 12 14 16
0 20 40 60 80 100 120 6.0 6.5 7.0 7.50 2 4 6 8 165 180 195 210 225
Sulfate conc. (mg/L)DMSO conc. (mg/L)
TIME (hr) pHDO (mg/L)ORP (mv)
Fig. 16、Effect of biomass on DMSO degradation using the immobilized cells of activated sludge.
Operational conditions: Temp.:30oC;
the rotational speed: 120 rpm; initial pH : 7.0;
biomass:
(—■—)0g; (—●●●●—)15g; (—▲—)30g;
(—▼—)45g; (—◆—)60g
0 5 10 15 20 25 0
100 200 300 400 3500 4000 4500 5000 5500 6000 6.0 6.5 7.0 7.5 0 2 4 6 8 10
concentration (mg/L)
TIME (hr) biomass (mg/L)pHDO (mg/L)
Fig. 17、Degradation of 200 mg/L DMSO by the suspend activated sludge as the sole source of carbon.
Operational conditions: initial pH: 7.0± 0.2
Temp.:30oC ;the rotational speed:120 rpm;
(————■————)DMSO; (————
●
————)Sulfate ion.0 20 40 60 80 100 0
200 400 600 800
DMSO concentration (mg/L)
TIME(hr)
Fig. 18、Effect of Dimethyl sulfoxide (DMSO) concentration on adsorption of medium and aeration using air.
Operational conditions:
initial pH:7.0± 0.2; Temp.: 30oC; air rate: 10L/min;
Initial DMSO concentration: 700 mg/L
0 50 100 150 200 250 300 0
100 200 300 400 500 600 700 800 900 1000
DMSO concertration (mg/L)
TIME (hr)
Fig. 19、DMSO biodegradation in air-lift bioreactor under repeated-batch mode.
Operational conditions:
initial pH: 7.0± 0.2; Temp.: 30oC;
air rate: 10L/min
0 20 40 60 80 100 120 140 160 0
200 400 600 800 1000 1200 2 3 4 5 6 7 8 5.5 6.0 6.5 7.0 7.5 8.0 8.5
DMSO concentration (mg/L)
TIME (hr) pHDO (mg/L)
Fig. 20、Repeated batch on DMSO degradation using the immobilized cells of activated sludge.
Operational conditions:
Temp.: 30oC; initial pH: 7.0± 0.2; air rate: 10L/min