Values are mean ± S. D. Control rats; Diabetes Mellitus (DM group) rats;
GABA Tea, diabetic with different dose GABA Tea rats for 3.01
μg/day/rat (DM-GABA-3 group) spp.; 30.1 μg/day/rat (DM-GABA-30 group) spp.; *P <0.05, ** P <0.01, *** P <0.001, significant differences between control group. #P <0.05, ## P <0.01, ### P <0.001, significant differences between DM group.
57
Fig 1.(A) The representative protein products of TNF-α and TNFR1 from cortex of brains in 4 groups, control, Diabetes Mellitus (DM) , DM + GABA tea-3, DM+GABA tea-30 were measured by Western blotting analysis.
-actin was use as a internal control.
(B)
Fig 1.(B) Bars represent the relative proteins quantification of TNF-α, TNFR1 based on β-actin and indicates the mean values ±SD(n=3). Values for DM sides were normalized to the corresponding control sides value. *** P<0.001.
** P<0.01. Values for DM+GABA tea-3 and DM+GABA tea-30 sides were normalized to the corresponding DM sides value . ### P<0.001. # P<0.05.
TNF-α
58
Fig 2.(A) The representative protein products of IL-6 from cortex of brains in 4 groups, control, Diabetes Mellitus (DM) , DM + GABA tea-3, DM+
GABA-30 tea were measured by Western blotting analysis. -actin was use as a internal control.
(B)
Fig 2.(B) Bars represent the relative proteins quantification of IL-6 based on β-actin and indicates the mean values ±SD(n=3). Values for DM sides were normalized to the corresponding control sides value.** P<0.01. Values for DM+GABA tea-3 and DM+GABA tea-30 sides were normalized to the corresponding DM sides value . ## P<0.01.
β-actin
59
Fig 3.(A) The representative protein products of p-JNK and p-NFκB from cortex of brains in 4 groups, control, Diabetes Mellitus (DM) , DM + GABA tea-3, DM+GABA tea-30 were measured by Western blotting analysis.
-actin was use as a internal control.
(B)
Fig 3.(B) Bars represent the relative proteins quantification of p-JNK and p-NFκB based on β-actin and indicates the mean values ±SD(n=3). Values for DM sides were normalized to the corresponding control sides value.***
P<0.001. ** P<0.01. Values for DM+GABA tea-3 and DM+GABA tea-30 sides were normalized to the corresponding DM sides value . ### P<0.001.
β-actin
60
Fig 4. (A) The representative protein products of Fas receptor and FADD extracted from cortex of brains in 4 groups, control, Diabetes Mellitus (DM) , DM + GABA tea-3, DM+GABA tea-30 were measured by Western blotting analysis. -actin was use as a internal control.
(B)
Fig 4. (B) Values for DM sides were normalized to the corresponding control sides value.*** P<0.001. * P<0.05. Values for DM+GABA tea-3 and DM+GABA tea-30 sides were normalized to the corresponding DM sides value . ### P<0.001. ## P<0.01.
61
Fig 5. (A) T The representative protein products of Caspase8 extracted from cortex of brains in 4 groups, control, Diabetes Mellitus (DM) , DM + GABA tea-3, DM+GABA tea-30 were measured by Western blotting analysis.
-actin was use as a internal control.
(B)
Fig5.(B) Bars represent the relative proteins quantification of cleaved-Caspase8 based on β-actin and indicates the mean values ±SD(n=3).
Values for DM sides were normalized to the corresponding control sides value.**P<0.01. Values for DM+GABA tea-3 and DM+GABA tea-30 sides were normalized to the corresponding DM sides value . ### P<0.001. # P<0.05.
β-actin 43 kDA
62
Fig 6. (A) The representative protein products of BH3 interacting domain death agonist (BID) and truncated BID (t-BID) extracted from cortex of brains in 4 groups, control, Diabetes Mellitus (DM) , DM + GABA tea-3, DM + GABA tea-30 were measured by Western blotting analysis. -actin was use as a internal control.
(B)
Fig 6.(B) Bars represent the relative proteins quantification of t-BID based on β-actin and indicates the mean values ±SD(n=3). Values for DM sides were normalized to the corresponding control sides value. **P<0.01. Values for DM+GABA tea-3 and DM+GABA tea-30 sides were normalized to the corresponding DM sides value . ## P<0.01. # P<0.05.
63
Fig 7. (A) The representative protein products of Bax and Cytochrome C extracted from cortex of brains in 4 groups, control, Diabetes Mellitus (DM) , DM + GABA tea, DM + 10x GABA tea were measured by Western blotting analysis. -actin was use as a internal control.
(B)
Fig 7. (B) Bars represent the relative proteins quantification of Bax and Cytochrome C based on β-actin and indicates the mean values ±SD(n=3).
Values for DM sides were normalized to the corresponding control sides value.**P<0.01. *P<0.05. Values for DM+GABA tea-3 and DM+GABA tea -30sides were normalized to the corresponding DM sides value . ## P<0.01.
Cytochrome C 11 kDA
43 kDA
64
Fig 8. (A) The representative protein products of Caspase-9 extracted from cortex of brains in 4 groups, control, Diabetes Mellitus (DM) , DM + GABA tea-3, DM+GABA tea-30 were measured by Western blotting analysis.
-actin was use as a internal control.
(B)
Fig 8. (B) Bars represent the relative proteins quantification of Caspase-9 based on β-actin and indicates the mean values ±SD(n=3). Values for DM sides were normalized to the corresponding control sides value.** P<0.01.
Values for DM+GABA tea-3 and DM+GABA tea-30 sides were normalized to the corresponding DM sides value . ## P<0.01. #P<0.05.
Cleaved-Caspase-9 35 kDA
57 kDA
65
Fig 9. (A) The representative protein products of cleaved-Caspase-3 extracted from cortex of brains in 4 groups, control, Diabetes Mellitus (DM) , DM + GABA tea-3, DM+GABA tea-30 were measured by Western blotting analysis.
-actin was use as a internal control.
(B)
Fig 9. (B) Bars represent the relative proteins quantification of Caspase-3 based on β-actin and indicates the mean values ±SD(n=3). Values for DM sides were normalized to the corresponding control sides value.** P<0.01.
Values for DM+GABA tea-3 and DM+GABA tea-30 sides were normalized to the corresponding DM sides value . ##P<0.01.
Caspase-3
66
Fig 10. (A) The representative protein products of Beclin-1 and ATG7 from cortex of brains in 4 groups, control, Diabetes Mellitus (DM) , DM + GABA tea-3, DM + GABA tea-30 were measured by Western blotting analysis.
-actin was use as a internal control.
(B)
Fig 10. (B)
Bars represent the relative proteins quantification of Beclin-1 and ATG7 based on β-actin and indicates the mean values ±SD(n=3). Values for DM sides were normalized to the corresponding control sides value.***
P<0.001.** P<0.01. Values for DM+GABA tea-3 and DM+GABA tea-30 sides were normalized to the corresponding DM sides value . ###P<0.001.##
P<0.01.
67
Fig 11.(A) The representative protein products of ATG12 from cortex of brains in 4 groups, control, Diabetes Mellitus (DM) , DM + GABA tea-3, DM + GABA tea-30 were measured by Western blotting analysis. -actin was use as a internal control.
(B)
Fig 11.(B) Bars represent the relative proteins quantification of ATG12 based on β-actin and indicates the mean values ±SD(n=3). Values for DM sides were normalized to the corresponding control sides value.** P<0.01. Values for DM+GABA tea-3 and DM+GABA tea-30 sides were normalized to the corresponding DM sides value . ### P<0.001.
ATG12 52 kDA
68
Fig 12.(A) The representative protein products of LC3 from cortex of brains in 4 groups, control, Diabetes Mellitus (DM) , DM + GABA tea-3, DM + GABA-30 tea were measured by Western blotting analysis. -actin was use as a internal control.
(B)
Fig 12. (B)
Bars represent the relative proteins quantification of Beclin-1 and ATG7 based on β-actin and indicates the mean values ±SD(n=3). Values for DM sides were normalized to the corresponding control sides value.**
P<0.01. Values for DM+GABA tea-3 and DM+GABA tea-30 sides were normalized to the corresponding DM sides value . ## P<0.01.
LC3Ⅰ
69
Fig 13.(A) The representative RNA products of Fas receptor and Caspase3 from cortex of brains in 4 groups, control, Diabetes Mellitus (DM) , DM + GABA tea-3, DM + GABA tea-30 were measured by RT-PCR analysis.
GAPDH was use as a internal control.
(B) Fig 13.(B) Bars represent the relative RNA quantification of Fas receptor and Caspase3 based on GAPDH and indicates the mean values ±SD(n=3). Values for DM sides were normalized to the corresponding control sides value.***
P<0.001. Values for DM+GABA tea-3 and DM+GABA tea-30 sides were normalized to the corresponding DM sides value . ### P<0.001.
Fas
70
Fig.14
Caspase-9
Fas rceptor FasL
FADD
Pro-caspase-8
Caspase-8
bid
t-bid
Bax
Cyt-c
＋Apaf‐1
Cyt-c
Caspase-3 Beclin-1
P-NFκB P-JNK
IL-6
Inflammation
ATG 12
LC3 Ⅱ LC3 Ⅰ
ATG 7
STZ