2 Faculty of Pharmacy, Kaohsiung Medical University, Kaohsiung 807, Taiwan
3 Graduate Institute of Pharmaceutical Technology & Department of Pharmacy, Tajen University, Pingtung 907, Taiwan
Pittosporum illicioides var. illicioides (Pittosporaceae) is an evergreen shrub that grows in medium-to-high altitude forests throughout China and Taiwan. Sesquiterpene glycosides, triterpenoid saponins, carotenoids, and their derivatives are widely distributed in plants of the genus Pittosporum. Many of these compounds exhibit diverse biological activities, including antimicrobial and cytotoxic activities. In our studies on the anti-inflammatory constituents of Formosan plants, many species have been screened for in vitro anti-inflammatory activity, and P. illicioides var. illicioides has been found to be one of the active species. Phytochemical investigation of the stem of this plant has led to the isolation of a new phthalide derivative, 3-ethyl-5,7- dihydroxy-6-methoxyisobenzofuran-1(3H)-one (1), along with six known compounds (2–7). The structural elucidation of new compound 1 is described herein.
Antioxidant effects of different solvent extracts of chamomile flower Kuo-Cheng Wang, Ming-Chi Hung, Yu-Dan Cheng I-Li Chen, Fu-An Chen,
Po-Chuen Shieh, Shu-Yu Li, Daih-Huang Kuo*
Graduate Institute of Pharmaceutical Technology, Tajen University, Pingtung, Taiwan
Chamomile (Matricariarecutita L.), a member of the daisy family, has been used as a herbal remedy for thousands of year. Recent studies have shown its carminative, stomachic, anti-inflammatory, anti-parasitic, anticancer and hypocholesteroemic properties. In this study, methanol was fully utilized to dissolve the active ingredients of chamomile flower, refluxed at 50℃ for 3 hours, concentrated under vacuum, and syrup product was archived (CME), then the syrup product was fractionated with n-hexane (CHE), dichloromethane (CDE), ethyl acetate (CEE) and water (CWE). DPPH scavenging effect and total phenol content were used to investigate their antioxidant capacity. Among these fractions, CEE contained components with the most effective DPPH scavenging potential and total phenol content. The DPPH free radical scavenging percentages were 12%, 67%, 95%, 75%, 39% and 63% for CHE, CDE, CEE, CME, CWE (1000μg/ml) and vitamin C (60μg/ml) that indicated CDE, CEE and CME (1000μg/ml) were superior to vitamin C (60μg/ml).The absorbances of total phenol content were 0.17, 0.71, 1.01, 0.54, 0.29 and 0.41 for CHE, CDE, CEE, CME, CWE (1000μg/ml) and gallic acid (160μg/ml) that displayed the total phenol contents of CDE, CEE, CME (1000μg/ml) were more than gallic acid (160μg/ml). These results suggest that chamomile flower is expected to be useful for antioxidant.
不同產地之中藥檳榔的品質評估
吳旻峰 1,2、何玉鈴3、黃冠中1、張永勳1,2*
1中國醫藥大學 中國藥學暨中藥資源學系
2中國醫藥大學附設醫院 藥劑部中藥局
3 弘光科技大學 護理學系
檳榔為棕櫚科Palmae植物檳榔Areca catechu的乾燥成熟種子，始載於名醫別 錄，列於草木部中品。歷代以降諸家本草，如新修本草、開寶本草、植物名實圖考 等均有著錄，為中醫臨床常用藥物之一，具破氣消積，化痰散痞之功效。海南、雲 南、印尼、台灣為其主要產地。檳榔富含檳榔鹼(Arecoline)、檳榔次鹼(Arecaidine)、
去 甲 基 檳 榔 次 鹼 (Guvacine) 、 去 甲 基 檳 榔 鹼 (Guvacoline) 等 生 物 鹼 與 Catechin 與 Epicatechin等黃酮類化合物。唯市售檳榔藥材外觀有大小、尖鈍、圓扁之不同，為確 保市售檳榔藥材之品質，進行本研究。
本研究收集上述三個不同產地的檳榔原藥材，以組織切片、粉末顯微鑑定、薄 層層析(TLC)、高效液相層析(HPLC)等鑑定方法，進行組織型態鑑定及生物鹼與黃酮 類的成分含量分析，並測定其對DPPH自由基清除能力、還原力、總多酚含量、類 黃酮含量及黃酮醇含量活性分析，以評估探討不同產地之檳榔間品質的差異。最後，
選擇中藥基準方200方中含檳榔之複方製劑「芍藥湯」，測定其散劑與濃縮顆粒劑 中檳榔鹼之含量多寡，探討以水為溶媒提取製成濃縮顆粒劑，對檳榔鹼之提取成 效。列出部分結果於下：不同產地檳榔之組織切片、粉末顯微鑑定皆相同；生物鹼 含量高低則有不同，其中，臺灣中藥典限定之檳榔鹼(Arecoline)含量以海南產最高為 0.611 ± 0.197%，其次為印尼產為0.526 ± 0.240%，雲南產最低為0.359 ± 0.070%，皆 符合臺灣中藥典檳榔鹼(Arecoline)含量不得少於0.20%之規範；檳榔次鹼(Arecaidine) 含量亦以海南產最高為0.102 ± 0.030%，其次為雲南產為0.039 ± 0.011%，印尼產最 低為0.015 ± 0.009%。海南、雲南、印尼三地所產之Catechin的含量分別為0.308 ± 0.049%、0.205 ± 0.040%、0.358 ± 0.125%；總多酚含量分別為113.736 ± 22.314%、
109.217 ± 25.837%、84.535 ± 34.478%。而對不同劑型之複方製劑，經水為溶媒提 取，對成品中檳榔鹼含量多寡之評估，尚在進行中。
藉由本研究，瞭解不同產地中藥檳榔之品質優劣，以海南所產之商品名為「海 南子」之品質較優，以此供臨床使用之質量參考。
New isoferulylglyceryl ester from Eriocaulon buergerianum Da-Rong Chen (陳達榮) and Jih-Jung Chen (陳日榮) *