Deng, Jeng-Shyan (鄧正賢)
Department of Health and Nutrition Biotechnology, Asia University
Massa medicata fermentata (Shenqu) consists of the mixture of fermented powders of wheat flour, Semen Armeniacae Amarum, sprout of Semen Phaseoli, Herba Artmisiae Annuae, Fructus Xanthii and Herba Polygoni Hydropiperis. Shenqu has sweet, pungent and warm properties, and attributive to the spleen and stomach meridians.
The actions of Shenqu is to promote digestion and harmonize the stomach.
Within the past two projects, we proceeded the above mycotoxin project to establish the method of analysis of Ochratoxin A in Chinese medicines. The method was applied to about 58 samples collected from retail shops in China and Taiwan, Ochratoxin A was detected in 52 samples of Massa Medicata Fermentata, measurable at 0.15-45.2 µg/kg. Evidence has suggested that mycotoxins contaminated in Shenqu produced in traditional processing, related to exposure fungal in fermented preparation. Shenqu was naturally contaminated by fungi that may become toxic for the human organism if the total amount ingested through consumption exceeds a certain tolerable dose. The quality management of Shenqu materials is very important for protectting consumer health. Therefore, a new process for developing Massa Medicata Fermentata was recommended in order to assess the optimal conditions for microbial fermentation of Shenqu.
The duration of this research project is approximately one years and divided into three parts.
1. The sub-subject I: To culture and determine the kinds of beneficial microorganism in Shenqu fermentation process.
The first part comprises the basic research dealing with identifing and classifing the microorganisms in Shenqu fermentation process. Beside, total plate count test were performed and yeast and mold testing represents the total fungi in sample.The results showed an enormous difference among the different batches of samples in the count of microorganisms.Twenty six of Shenqu were analysed in order to determine the microbial contamination level. The total viable aerobic count of Shenqu varied from 1.7×102-9.0×107 CFU/g and in 11 samples out of 26 was equal to or higher than 107 CFU/g. Rate of unqualified of the total viable aerobic count of Shenqu was 42.3 ％.
Total yeasts and molds count of Shenqu varied from 1×101-1.1×107 CFU/g and in 8 samples out of 26 was equal to or higher than 104 CFU/g. Rate of unqualified of the total yeasts and molds count of Shenqu was 30.7％.Microorganisms in the SQTCM Shenqu (No. 21) Sample collected from Sichuan sources were separated and identified. Three strains of microorganisms were isolated .2. The sub-subject II: To evaluate the effect of isolated unidentified strains and pure inoculation fermentation
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The objective of sub-subject II was to evaluate the effect of isolated unidentified strains on promoting digestion. The active ingredients evaluated were lipase activity, protease activity and amylase activity, and screen out the microorganisms for the presence of aflatoxins.
In this survey, 26 samples were purchased in Mainland, Taiwan and Japan in order to investigate the aflatoxins contamination and evaluate the enzyme activity in Shenqu.
The samples were analyzed of aflatoxins using LC with Post-Column Photochemical Derivatization. The method was applied to 26 samples and aflatoxins were detected in 12 samples, measurable at 1.5-76.8 µg/kg, 5 samples exceed standard requested of the total aflatoxin 15μg/kg.
For selecting better fermentation strains, the quality of various Shenqu was evaluated by lipase activity, protease activity and amylase activity. The enzyme activities were found in 24 samples, measurable at 2.2-89.5 U/g (Amylase), 0.8-49.8 mU/g (Protease) and 7.3-331.2 (mU/g) (lipase). With regard to amylase activity and lipase, the SQTCM Shenqu (No. 21) demonstrated higher activity than the others.
3. The sub-subject III: DNA sequencing analysis for the species identification of isolated microorganisms
Identification of Shenqu important microorganisms by ITS sequencing, especially using the ITS region, is reliable and can be used as an accurate alternative to conventional identification methods. Three strains of microorganisms isolated from the SQTCM Shenqu (No. 21), they are Bacillus safensis (Bacillaceae) and Lichtheimia ramosa (Mucoraceae).
Keywords：
Massa Medicata Fermentata ,Shenqu, aflatoxins, lipase, protease, amylase35
鄧正賢 助理教授簡介 姓名：鄧正賢
地址：Department of Health and Nutrition Biotechnology, Asia University, 500 Lioufeng Road, Wufeng, Taichung 41354, Taiwan, ROC
電話：886 + (0)4 + 2332-3456 ext. 1836 E-mail address: [email protected]
現職：亞洲大學保健營養生技學系 助理教授
鄧正賢，專長為層析分析分離技術及保健食品開發，十一 年來在教學研究工 作上積極投入，帶 領學生從事台灣本土植物資源暨天然保健食品之生物活性篩選
建立平台，連續三年獲得亞洲大學學術卓越研究獎及亞洲大學 102 年度學術成
果豐碩獎，持續發表並進行台灣本土植物之活性研究。此外並獲得99年度亞洲大
學「獎助創新教材獎」、98年度亞洲大學「獎助創新教材獎」、102年度亞洲大
學產學合作獎。
Publications in Last Five Years
Jeng-Shyan Deng, Shyh-Shyun Huang, Tsung-Hui Lin, Min-Min Lee , Ching-Chuan Kuo, Ping-Jyun Sung, Wen-Chi Hou, Guan-Jhong Huang , Yueh-Hsiung Kuo. The analgesic and anti-inflammatory bioactivities of eburicoic acid and dehydroeburicoic acid isolated from Antrodia camphorata on the inflammatory mediator expression in mice. Journal of Agricultural and Food Chemistry, 2013; DOI: 10.1021/jf303820k.
Publication Date (Web): March 15, 2013. (SCI )
Shyh-Shyun Huang, Hsien-Jung Chiu, Tsung-Hui Lin, Min-Min Lee, Chao-Ying Lee, Shu-Jen Chang, Wen-Chi Hou, Guan-Jhong Huang, Jeng-Shyan Deng. Antioxidant and Anti-inflammatory Activities of Aqueous Extract of Centipeda minima. Journal of Ethnopharmacology, 2013, DOI: http://dx.doi.org/10.1016/j.jep.2013.03.025.
Received date: 13 December 2012. Revised date: 6 March 2013. Accepted date: 8 March 2013. (SCI )
Guan-Jhong Huang, Shyh-Shyun Huang, Jeng-Shyan Deng＊. Anti-Inflammatory Activities of Inotilone from Phellinus linteus through the Inhibition of MMP-9, NF-κB, and MAPK Activation In Vitro and In Vivo. PLoS One. 2012 May, vol. 7, issue 5, p. e35922. (SCI )
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Deng-Shyan Deng, Chuan-Sung Chi, Shyh-Shyun Huang, Pei-Hsin Shie, Tsung-Hui Lin, Guan-Jhong Huang. Antioxidant, Analgesic, and Anti-inflammatory activities of the ethanolic extracts of Taxillus liquidambaricola. Journal of Ethnopharmacology, 2011 Oct 11;137(3):1161-71. (SCI )
Guan-Jhong Huang, Shyh-Shyun Huang, Pei-Hsin Shie, Tsung-Hui Lin, Jeng-Shyan Deng. Anti-inflammatory activities of aqueous extract of Mesona procumbens in experimental mice, Journal of the Science of Food and Agriculture - 2012 Apr;
92(6):1186-93. (SCI )
Jeng-Shyan Deng, Yi-Chih Chang, Chi-Luan Wen, Jung-Chun Liao, Wen-Chi Hou, Sakae Amagaya,Shyh-Shyun Huang, Guan-Jhong Huang. Hepatoprotective effect of the ethanol extract of Vitis thunbergii on carbon tetrachloride-induced acute hepatotoxicity in rats through anti-oxidative activities，Journal of Ethnopharmacology, 2012 Aug 1;142(3):795-803. (SCI )
Guan-Jhong Huang, Jeng-Shyan Deng(co-first author), Shyh-Shyun Huang, Chao-Ying Lee, Wen-Chi Hou, Sheng-Yang Wang, Ping-Jyun Sung, Yueh-Hsiung Kuo. Hepatoprotective effects of eburicoic acid and dehydroeburicoic acid from Antrodia camphorata in a mouse model of acute hepatic injury.
Food Chemistry, 2013.03.18 Accept. (SCI )
Ming-Jyh Sheu, Jeng-Shyan Deng (co-first author), Ming-Hsing Huang, Jung-Chun Liao, Chieh-Hsi Wu, Shyh-Shyun Huang, Guan-Jhong Huang. Antioxidant and anti-inflammatory properties of Dichondra repens Forst. and its reference compounds.
Food Chemistry, Volume 132, Issue 2, 15 May 2012, Pages 1010–1018. (SCI )
Huang Guan-Jhong, Jeng-Shyan Deng (co-first author), Hsien-Jung Chen, Shyh-Shyun Huang, Jung-Chun Liao, Wen-Chi Hou, Yaw-Huei LIN. Defensin protein from sweet potato (Ipomoea batatas [L.] Lam ‘Tainong 57’) storage roots exhibits antioxidant activities in vitro and ex vivo. Food Chemistry, 2012 Apr, 135:861-867. (SCI ) (Impact factor 3.655，5-Year Journal Impact Factor 4.268，
ranking 6/128=4.6%, 2011 JCR)
Guan-Jhong Huang, Jeng-Shyan Deng(co-first author), Jung-Chun Liao, Wen-Chi Hou, Sheng-Yang Wang, Ping-Jyun Sung, and Yueh-Hsiung Kuo. Inducible Nitric Oxide Synthase and Cyclooxygenase-2 Participate in Anti-inflammatory Activity of Imperatorin from Glehnia littoralis. Journal of Agricultural and Food Chemistry,
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2012 Feb 22; 60(7):1673-81. (SCI )
Jung-Chun Liao, Jeng-Shyan Deng(co-first author), Chuan-Sung Chiu, Shyh-Shyun Huang, Wen-Chi Hou, Wang-Ching Lin, Guan-Jhong Huang. Chemical compositions, Anti-inflammatory, Antiproliferative and Radical-scavenging Activities of the methanol extracts of Actinidia callosa var. ephippioides，AMERICAN JOURNAL OF CHINESE MEDICINE，2012; 40(5):1047-62. (SCI )
Guan-Jhong Huang, Jeng-Shyan Deng(co-first author), Hsien-Jung
Chen, Shyh-Shyun Huang, Chun-Ching Shih and Yaw-Huei Lin. Dehydroascorbate reductase and monodehydroascorbate reductase activities of two metallothionein-like proteins from sweet potato (Ipomoea batatas [L.] Lam. ‘Tainong 57’) storage roots.
Received:2 May 2013 Accepted:26 June 2013. Botanical Studies, 2013. (SCI ) Jung-Chun Liao, Jeng-Shyan Deng, Chuan-Sung Chiu, Shyh-Shyun Huang, Wen-Chi Hou, Wang-Ching Lin, Guan-Jhong Huang. Chemical compositions,
Anti-inflammatory, Antiproliferative and Radical-scavenging Activities of the
methanol extracts of Actinidia callosa var. ephippioides，AMERICAN JOURNAL OF CHINESE MEDICINE， 2012; 40(5):1047-62. (SCI )
Chia-Yu Liu, Ying-Chih Lin, Jeng-Shyan Deng, Jung-Chun Liao, Wen-Huang Peng and Guan-Jhong Huang. Antioxidant, Anti-inflammatory, and Antiproliferative Activities of Taxillus sutchuenensis，AMERICAN JOURNAL OF CHINESE MEDICINE. 2012 Mar, 40(2):335-348. (SCI )
Liao Jung-Chun, Jeng-Shyan Deng, Chao-Ying Lee, Min-Min Lee, Wen-Chi Hou, Shyh-Shyun Huang, Guan-Jhong Huang and Ying-Chih Lin. Antioxidant,
Anti-nociceptive, and Anti-inflammatory Activities from Actinidia callosa var. callosa in vitro and in vivo. Evidence-Based Complementary and Alternative Medicine. (has now been accepted for publication) (SCI )
Tien-Ning Chang, Jeng-Shyan Deng, Yi-Chih Chang, Chao-Ying Lee, Liao Jung-Chun, Min-Min Lee, Wen Huang Peng, Shyh-Shyun Huang, and
Guan-JhongHuang. Ameliorative Effects of Scopoletin from Crossostephium chinensis against Inflammation Pain and Its Mechanisms in Mice. Volume 2012, Article ID 595603, 10 pages. doi:10.1155/2012/595603. Evidence-Based
Complementary and Alternative Medicine. (SCI )
Jung-Chun Liao, Jeng-Shyan Deng, Chuan-Sung Chi, Wen-Chi Hou, Shyh-Shyun
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Huang, Pei-Hsin Shie, Huang Guan-jhong. Anti-inflammatory Activities of Cinnamomum cassia Constituents in vitro and in vivo. Evidence-based Complementary and Alternative Medicine, 2012 March 27.
doi: 10.1155/2012/429320. (SCI )
Shu-Ling Liu, Jeng-Shyan Deng, Chuan-Sung Chiu, Wen-Chi Hou, Shyh-Shyun Huang, Wang-Ching Lin, Jung-Chun Liao, Huang Guan-Jhong. Involvement of heme oxygenase-1 participate in anti-inflammatory and analgesic effects of aqueous extract of Hibiscus taiwanensis. Evidence-based Complementary and Alternative Medicine.
2012;2012:132859. Epub 2012 Jun 21. (SCI )
Huang Guan-Jhong, Jeng-Shyan Deng, Shyh-Shyun Huang, Yi-Yuan Shao, Chin-Chu Chen, Yueh-Hsiung Kuo. Protective effect of antrosterol from Antrodia camphorata submerged whole broth against carbon tetrachloride-induced acute liver injury in mice. Food Chemistry, 2012 May, 132(2):709-716. (SCI )
Guan-Jhong Huang, Jeng-Shyan Deng, Shyh-Shyun Huang, and Miao-Lin Hu.
Hispolon Induces Apoptosis and Cell Cycle Arrest of Human Hepatocellular Carcinoma Hep3B Cells by Modulating ERK Phosphorylation. Journal of Agricultural and Food Chemistry. 2011 Jul 13;59(13):7104-13. (SCI ) Guan-Jhong Huang, Jeng-Shyan Deng, Chuan-Sung Chiu, Jung-Chun
Liao,Wen-Tsong Hsieh, Ming-Jyh Sheu, and Chieh-HsiWu. Hispolon protects against acute liver damage in the rat by inhibiting lipid peroxidation, proinflammatory
cytokine, oxidative stress and down-regulating the expressions of iNOS, COX-2, MMP-9. Evidence-based Complementary and Alternative Medicine,
2012 Mar,Volume 2012, Article ID 480714, 12 pages. (SCI )
Guan-Jhong Huang, Jeng-Shyan Deng, Hsien-Jung Chen, Shyh-Shyun Huang, Chieh-Hsi Wud, Jung-Chun Liao, Shu-Jen Chang, Yaw-Huei Lin, Inhibition of reactive nitrogen species invitro and exvivo by thioredoxin h2 from sweet potato
‘Tainong 57’storage roots. Food Chemistry, Volume 131, Issue 2, 15 March 2012, Pages 552–557. (SCI )
Guan-Jhong Huang, Jeng-Shyan Deng, Shyh-Shyun Huang, Chi-I Chang, Tien-Ning Chang, Pei-Hsin Shie, and Yueh-Hsiung Kuo. Anti-inflammatory Activities of 6β -Acetoxy-7r-hydroxyroyleanone 2 from Taiwania cryptomerioides Hayata ex Vivo and in Vivo. Journal of Agricultural and Food Chemistry, 2011, 59 (20), pp
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11211–11218. (SCI )
Yu-Ling HO, Shyh-Shyun HUANG, Jeng-Shyan DENG, Yaw-Huei LIN,Yuan-Shiun CHANG, Guan-Jhong HUANG. In vitro antioxidant properties and total phenolic contents of wetland medicinal plants in Taiwan. Bontanical Studies, 2012,
53(1):55-66. January. (SCI )
Tien-Ning Chang, Shyh-Shyun Huang, Yuan-Shiun Chang, Chi-I Chang, Hsin-Ling Yang, Jeng-Shyan Deng, Yueh-Hsiung Kuo,and Guan-Jhong Huang. Analgesic Effects and Mechanisms of Anti-inflammation of Taraxeren-3-one from Diospyros maritima in Mice. Journal of Agricultural and Food Chemistry, 2011, 59 (17):
9112–9119. (SCI )
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