Conclusion:
D, in vivo MSOT images were further validated through fluorescence imaging detection of
EGF-750 probe in pancreatic tumor and liver tissues analyzed ex vivo on the AMI; ex vivo scan of organs con fi rmed the accumulation in the
pancreas and lack of fluorescent EGF-750 probe distribution in the liver, corresponding to MSOT signal quantified for the liver and
kidney at < 10 MSOT signal units (a.u.).
Comparison of High Sensitivity BLI Imaging Systems for Ultra-weak Signal Applications
High sensitivity BLI image taken with an IVIS 200 and Ami X system. The same C57BL/6 LSL-luciferase TP53fl/fl PTENfl/fl mouse 16 weeks post-viral exposure was imaged following D-luciferin intraperitoneal
injection
Center vs. Corner Measurements for Three High Sensitivity BLI Systems
Corner vs. Center Measurements
All three instruments achieve color range required for high sensitivity BLI imaging, but IVIS 200 and Ami X lenses require less correction resulting in no halo
AmiX 觀察螢光探針Cy7在腫瘤裸鼠體內的螢光成像
注射前 注射後15min 注射後30min
注射後1h 注射後2h
注射2h後犧牲老鼠取出器 官及腫瘤組織成像圖
肺 心
肝 脾
腫瘤 胃腸
腎臟
※ 螢光物質在注射後的30min時間，於 腫瘤處的攝取值達到最高峰，而身體 其他地方的攝取則則相對低許多，顯 示此螢光物質具有高度的靶向性。
※ 注射後2h犧牲老鼠後，取出腫瘤及身
體其他組織器官比較的結果，顯示腫
瘤處的確擁有非常高的螢光信號，與
螢光活體影像的結果一致性的吻合。
Cerenkov Luminescence Imaging
Visual analysis yielded a spatial resolution of 1.2 mm.
Linearity was assessed by placing samples of varying activities in the AMI-1000 and
The CLI image and the PET image both show uptake in the spine and growth plates in the knees.
Cerenkov radiation: It is possible that the beta particle (electron or positron) is relativistic, traveling
faster than the speed of light (v>c/n) in the tissue.
＊ c= speed of light in a vacuum
＊ n= index of refraction of the medium
AmiX 阿黴素體內代謝的螢光成像
對照組小鼠 注射阿霉素後24h影像 小鼠解剖影像
對照小鼠在阿黴素注射前先進行影像掃描擷取，會發現其腋下和下腹部出現訊號，判定為小鼠的自發螢光現象。在通過尾 靜脈注射阿黴素後約24小時成像，發現了上腹部出現了明顯的螢光訊號。藉由動物解剖結果得知，上腹部的螢光訊號主要 來自於膽囊，與阿黴素主要通過膽汁排泄的代謝路徑相符合。由上述影像結果可以清楚看出，使用新型LED燈做為激發光，
老鼠自我產生的自發螢光是極低的，因此不會影響實驗結果及分析數據。
Ex: 465 nm
Em: 630 nm
Scan time: 1s
AmiX 觀察ZnPcG4在裸鼠體內的螢光成像
注射ZnPcG4前 注射後10min影像 注射後1h影像
注射後3h影像 注射後5h影像 注射後6h影像
注射ZnPcG4後於老鼠體內代謝分布的結果。將各個時間點的color map以統一的方式呈現，從影像中可以清楚 看到注射了ZnPcG4後，10min-1h的胸部訊號最強；注射後3h胸部訊號逐漸減弱直到6h後胸部訊號完全消失。
在6h的主要訊號經由X光結果證實來自於腎臟。
注射後6h影像 (俯臥位)
腿部骨腫瘤 大視野X光成像小鼠影像
小視野X光成 像後小鼠影像
AmiX系統X光成像小鼠結果
Photograph overlay X-ray overlay
Images of mice 48 hours post injection of OsteosenseTM 680
SI Imaging 靈敏度最好的活體成像系統
Detect Fewer Cells
Lago Minimum Detectable Radiance is
45 photons/sec/cm2/sr.The Lago specification is 36% better than the IVIS
®Spectrum specification of 70 photos/sec/cm
2/sr. Industry Leading Field of View
High Throughput
Lowest Background Signal
High Performance -90° C Absolute Camera
No External Coolers
Completely Air Cooled
Fourteen LED’s for Fluorescence Excitation
Wavelengths of 360, 405, 430, 465, 500, 535, 570, 605, 640, 675, 710, 745, 770 and 805nm are standard for excitation of fluorescence species
Twenty Emission Filters for Fluorescence and Luminescence Imaging