β-carotene prevents hepatic lipid accumulation in rats under chronic alcohol consumption.

β-胡蘿蔔素抑制長期攝食酒精之大白鼠肝臟脂肪堆積ββ

-carotene prevents hepatic lipid accumulation in rats under

chronic alcohol consumption

朱娟秀

Huang CC;Chen JR;Huang TI;Shieh MJ;Chu JS;Yang SC

摘要 本實驗之目的在探討自β-胡蘿蔔素對於長期攝取酒精所導致大白鼠高三酸甘油 酯血症以及酒精性脂肪肝之影響。以雄性 SD 大白鼠為實驗動物，依肝功能指標 GOT 興 GPT 活性分成三組：控制組、酒精組以及β-胡蘿蔔素添加酒精組，實驗 期 10 週。結果顯示：血漿中 GOT 與 GPT 活性方面，酒精組在第 6、8、10 週時 均顯著較控制組上升，而β-胡蘿蔔素添加酒精組在第 10 週時則是均顯著較酒精 組降低。血漿中總膽固醇濃度方面，酒精組在第 10 適時明顯較其他二組減少。 血漿中三酸甘油酯濃度方面，酒精組則是在第 4、6、8、10 週時明顯較其他二組 增加。此外，β-胡蘿蔔素添加酒精組，血漿中高密度脂蛋白膽固醇濃度在第 10 週時顯著較酒精組增加 13%。至於肝中膽固醇與三酸甘油酯含量方面，酒精組較 控制組分別增加 33%與 73%，而β-胡蘿蔔素添加酒精組則是分別較酒精組減少 20%及 38%。另外，β-胡蘿蔔素添加酒精組大白鼠肝臟中可測得β胡蘿蔔素，而 且β-胡蘿蔔素添加酒精組肝臟中維生素 A 之含量分別較控制組與酒精組增加 33%與 51%。由肝臟組織病理切片觀察顯示，酒精組大白鼠肝臟中有脂質堆積的 現象，而β-胡蘿蔔素添加酒精組大白鼠則無此現象。由以上結果可知，補充β-胡蘿蔔素可以減少血漿中三酸甘油酯濃度，以及肝臟中膽固醇與三酸甘油酯之含 量，進而預防酒精性脂肪肝之形成。 Abstract

The purpose of this study was to investigate the effects of β-carotene on hypertriglycemia and alcoholic fatty liver in rats with chronic alcohol intake. Rats were divided into three groups: the control (c), ethanol (E), and ethanol with β-carotene (E + B) groups. After 10 weeks, results revealed that plasma GOT and GPT activities in group E were significantly higher than those in group C at weeks 6, 8, and 10, but they were significantly lower in group E + B compared to group E at week 10. Plasma total cholesterol (TC) levels in group E were significantly lower than those in the other groups at week 10. When

compared to the plasma triglyceride (TG) concentration in group E, those of groups C and E + B were significantly lower at weeks 4, 6, 8, and 10. Furthermore, plasma HDL-C levels in group E + B were significantly higher, by 13 %, than those in group E at week 10.

On the other hand, the hepatic cholesterol and TG contents of group E were significantly higher, by 33% and 73%, respectively, than those in C group. However, hepatic cholesterol and TG contents in group E + B were significantly lower by 20% and 38%, respectively, compared to those in group E. β-carotene storage was detected in livers of E + B group, and the hepatic retinal content was significantly higher than those in groups C and E by 33% and 51 %, respectively. Furthermore, the apparent accumulation of fat within hepatocytes was observed in the ethanol group. Results demonstrate that β-carotene supplementation can prevent alcoholic fatty liver formation by decreasing the plasma TG concentration, and inhibiting the accumulation of cholesterol and TG contents in the liver.