Accession number:20091412006742
Title:Development of a novel BIA enzyme calorimetric biosensor and detection system for hydrogen peroxide determination at hazardous level
Authors:Wei, Hsien-Chin (1); Huang, Su-Hua (2); Lee, Yeun-Chung (1) Author affiliation:(1) National Taiwan University, Department of Bio- Industrial Mechatronics Engineering, No. 1, Roosevelt Road, Taipei, 10617, Taiwan; (2) Asia University, College of Health Science,
Department of Biotechnology, 500, Lioufeng Rd., Wufeng, Taichung 41354, Taiwan
Corresponding author:Wei, H.-C.
Source title:American Society of Agricultural and Biological Engineers Annual International Meeting 2008
Abbreviated source title:Am. Soc. Agric. Biol. Eng. Annu. Int. Meet.
Volume:5
Monograph title:American Society of Agricultural and Biological Engineers Annual International Meeting 2008
Issue date:2008
Publication year:2008 Report number:084035 Pages:3061-3071
Language:English
ISBN-13:9781605605364
Document type:Conference article (CA)
Conference name:American Society of Agricultural and Biological Engineers Annual International Meeting 2008
Conference date:June 29, 2008 - July 2, 2008 Conference location:Providence, RI, United states Conference code:75694
Publisher:American Society of Agricultural and Biological Engineers, 2950 Niles Road, St. Joseph, MI 49085-9659, United States
Abstract:This paper presents a calorimetric biosensor and detection system based on a medical thermistor and a disposable polymeric reaction cell for detecting hydrogen peroxide in a batch- injection- analysis (BIA) process. Single thermistor was used as a calorimetric sensing element to measure temperature change in an enzyme-
substrate exothermic reaction. The detection system consists of a transducer, an analog-to-digital conversion device with 16-bits resolution, a single chip microcontroller, and a universal-serial-bus (USB) interface to communication with personal computer. A
computer program for dynamic data acquisition and recording was developed to monitor and record temperature profile of the
calorimetric biosensor during reaction. Disposable PMMA polymeric cell was used as a reaction cell to fill enzyme and the reaction
medium. As results, after linearization of calorimetric biosensor and primary grade calibration, the accuracy of temperature
measurement for the developed system is ± 0.001 °C and correlation coefficient R<sup>2</sup> is 0.999 at temperature between 20 and 30 °C. When the calorimetric biosensor was incorporated with the detection system, it showed a linear range between 3.125 x 10&<sup>-2</sup> M to 1 M for hydrogen peroxide concentration detection with a correlation coefficient R<sup>2</sup> of 0.9991. Compare with other types of hydrogen peroxide sensor and detection systems, the developed BIA enzyme calorimetric biosensor and detection system has a potential of low cost and high precision for hydrogen peroxide determination for the concentration at toxic and hazardous level. The system could be applied further for other biocalorimetry researches.
Number of references:17 Main heading:Biosensors
Controlled terms:Calorimetry - Enzymes - Functional polymers - Hydrogen - Hydrogen peroxide - Interfaces (computer) - Oxidation - Personal computers - Pumps - Signal transduction - Temperature measurement - Thermal effects - Thermistors
Uncontrolled terms:Analog-to-digital conversions - Batch-injection- analysis (BIA) - Biocalorimetry - Calorimetric biosensor - Calorimetric sensing - Computer programs - Correlation coefficients - Detection systems - High precision - Hydrogen peroxide concentrations - Hydrogen peroxide sensors - Linear ranges - Low costs - Medical thermistor - Polymeric reactions - Reaction cells - Reaction mediums - Serial bus - Single-chip microcontrollers - Temperature changes - Temperature profiles
Classification code:722.4 Digital Computers and Systems - 732 Control Devices - 801.2 Biochemistry - 802.2 Chemical Reactions -
951 Materials Science - 804 Chemical Products Generally - 804.2 Inorganic Compounds - 815.1 Polymeric Materials - 944.6
Temperature Measurements - 804.1 Organic Compounds - 722.2 Computer Peripheral Equipment - 714.2 Semiconductor Devices and Integrated Circuits - 421 Strength of Building Materials; Mechanical Properties - 461.2 Biological Materials and Tissue Engineering - 461.8 Biotechnology - 461.9 Biology - 462.1 Biomedical Equipment, General - 531 Metallurgy and Metallography - 618.2 Pumps - 641 Heat and Mass Transfer; Thermodynamics
Database:Compendex
Compilation and indexing terms, Copyright 2009 Elsevier Inc.
