Gene 1
Proteoglycan 1 Gene 2
CD53 antigen
Gene 3
Proteosome subunit
Meaningful expression data based on specific cell types.
Histochem J., Vol 8, Issue 4, 387 - 401, 1976
Science
Proteomics, 6: 2259, 2006
Introducing Arcturus
™Microdissection Instrument
Open, modular design platform for maximum research flexibility
LCM – near IR UV laser cutting
Image analysis (MetaMorph) Laser tweezers
Environmental control More sample formats
Arcturus
™based on Nikon TE2000 Research Microscope
Modularity and Expandability
Environmental Control Incubator
Arcturus graphical user interface
Microdissect Tools Panel
Object Color
Texture
Cell Recognition
Cell Recognition
Image Registration
Image Registration
CapSure
Tissue Glass Slide IR Laser LCM
UV Laser Cutting and Ablation
IR Laser LCM IR Laser LCM
CapSure CapSure
Tissue Tissue
UV Laser Cutting
and Ablation UV Laser Cutting
and Ablation Membrane
Slide
Frame slide
Glass Slide Membrane
Ultimate sample preparation flexibility
Arcturus laser cutting – live plant microdissection
Whole mount
preparation. Blade of grass on frame
membrane slide.
Drosophila Embryo X-gal-Stained
Fluorescence
Arcturus fluorescence microdissection
FFPE Human Breast Tissue
Arcturus - large area dissection
Application
Some Topics of Interest
Sequence
PCR, QPCR
Mass Spec
2D Gel Microarray
• Approximately 2000 peer-reviewed articles
Applications
Proteomic Analysis of Alzheimer’s Disease
Journal of Biological Chemistry 279: 37061-37066 (2004)
Genesis, 2004, 39: 58-64
Laser Capture
Laser Capture Microdissection Microdissection of Fluorescently of Fluorescently
Labeled Embryonic Cranial Neural Crest Cells
Labeled Embryonic Cranial Neural Crest Cells
Forensic Applications of LCM Forensic Applications of LCM
Applications
Molecular
Molecular Classifcation Classifcation of 39 Cancer Subtypes: of 39 Cancer Subtypes:
Diagnosis of Carcinoma of Unknown Primary (CUP) Diagnosis of Carcinoma of Unknown Primary (CUP)
11th Annual Meeting of the Association for Molecular Pathology November 11, 2005
Developed a 92 gene PCR-based assay for the classification of 39 tumor types
A two-gene expression ratio predicts clinical outcome in breast cancer patients treated with
tamoxifen
Tamoxifen
• Antiestrogen agent
• early stage, matastasis, ER+
• 25% ER+/PR+, 66% ER+/PR-, 55% ER/PR+ tumors fail to respond
• The mechanisms remain largely
A two-gene expression ratio of HOXB13 and IL-17BR for prediction of recurrence and survival in women receiving adjuvant tamoxifen.
256 pts received 5 years of tamoxifen monotherapy
J Clin Oncol 25:662-668, 2007
independent 1,252 patient retrospective clinical validation study
