Author(s): Chan, KC (Chan, Kung-chi); Mong, MC (Mong, Mei-chin); Yin, MC (Yin, Mei-chin) Title: Antioxidative and Anti-Inflammatory Neuroprotective Effects of Astaxanthin and Canthaxanthin in Nerve Growth Factor Differentiated PC12 Cells
Source: JOURNAL OF FOOD SCIENCE, 74 (7): H225-H231 SEP 2009 Language: English
Document Type: Article
Author Keywords: anti-inflammatory; antioxidative; astaxanthin; canthaxanthin; membrane stability; neurodegeneration; neuroprotection; PC12 cells
KeyWords Plus: OXIDATIVE STRESS; PARKINSONS-DISEASE; MITOCHONDRIAL DYSFUNCTION; INFLAMMATORY MARKERS; RAT-BRAIN; NEUROTOXICITY;
APOPTOSIS; ACTIVATION; EXPRESSION; STRIATUM
Abstract: Nerve growth factor differentiated PC12 cells were used to examine the
antioxidative and antiinflammatory effects of astaxanthin (AX) and canthaxanthin (CX). PC12 cells were pretreated with AX or CX at 10 or 20 mu M, and followed by exposure of hydrogen peroxide (H2O2) or 1-methyl-4-phenylpyridinium ion (MPP+) to induce cell injury. H2O2 or MPP+ treatment significantly decreased cell viability, increased lactate dehydrogenase (LDH) release, enhanced DNA fragmentation, and lowered mitochondrial membrane potential (MMP) (P < 0.05). The pretreatments from AX or CX concentration-dependently alleviated H2O2 or MPP+-induced cell death, LDH release, DNA fragmentation, and MMP reduction (P < 0.05).
Either H2O2 or MPP+ treatment significantly increased malonyldialdehyde (MDA) and reactive oxygen species (ROS) formations, decreased glutathione content, and lowered glutathione peroxidase (GPX) and catalase activities (P < 0.05). The pretreatments from AX or CX significantly retained GPX and catalase activities, and decreased MDA and ROS formations (P
< 0.05). H2O2 or MPP+ treatment significantly decreased Na+-K+-ATPase activity, elevated caspase-3 activity and levels of interleukin (IL)-1, IL-6, and tumor necrosis factor (TNF)-alpha (P < 0.05); and the pretreatments from these agents significantly restored Na+-K+-ATPase activity, suppressed caspase-3 activity and release of IL-1, IL-6, and TNF-alpha (P < 0.05).
Based on the observed antioxidative and anti-inflammatory
Addresses: [Yin, Mei-chin] China Med Univ, Dept Nutr, Taichung, Taiwan; [Chan, Kung-chi]
Providence Univ, Dept Food & Nutr, Taichung Cty, Taiwan; [Mong, Mei-chin] Asia Univ, Dept Hlth & Nutr Biotechnol, Taichung Cty, Taiwan
Reprint Address: Yin, MC, China Med Univ, Dept Nutr, Taichung, Taiwan.
E-mail Address: [email protected] Funding Acknowledgement:
Funding Agency Grant Number
China Medical Univ., Taichung City, Taiwan CMU97-203
This study was supported by a grant from China Medical Univ., Taichung City, Taiwan, ROC (CMU97-203).
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Times Cited: 0
Publisher: WILEY-BLACKWELL PUBLISHING, INC
Publisher Address: COMMERCE PLACE, 350 MAIN ST, MALDEN 02148, MA USA ISSN: 0022-1147
DOI: 10.1111/j.1750-3841.2009.01274.x 29-char Source Abbrev.: J FOOD SCI ISO Source Abbrev.: J. Food Sci.
Source Item Page Count: 7
Subject Category: Food Science & Technology ISI Document Delivery No.: 490JY
