白藜蘆醇對綠膿桿菌之抑制效果研究 黃彥能、張耀南
E-mail: [email protected]
摘 要
本研究將市售50%與98%純度之白藜蘆醇 ( resveratrol, 3,5,4’-trihydroxystilbene ) 分別溶於含有不同濃度DMSO ( Dimethyl sulfoxide ) 之 10% 乙醇溶液中，再進行其對綠膿桿菌( Pseudomonas aeruginosa ) 之抑菌效果探討。研究結果顯示，50% 與 98% 純度白藜蘆醇對綠膿桿菌具有相似之抑菌效果，一般而言，98% 純度白藜蘆醇的抑菌效果大於 50% 純度者，但實際 上其差異性不顯著，在第 4 小時培養時間時，其抑菌效果達到最佳狀態，但與白藜蘆醇的添加濃度與純度似乎無任何關係
；白藜蘆醇的抑菌效果隨即降低，在第 8~12 小時培養時間時，其抑菌效果為最差，而後其抑菌效果逐漸增加而至穩定狀 態。另外，DMSO 添加濃度亦影響白藜蘆醇的抑菌效果，其抑菌效果在 0.8% DMSO 添加濃度下達到最佳狀態，但隨其添 加濃度增加而有少許下降趨勢。最後，若以 1.0 mg/ml 白藜蘆醇添加於含有 0.8% DMSO 之 10% 乙醇溶液中，白藜蘆醇對 綠膿桿菌的抑菌效果可能達到最佳狀態。
關鍵詞 : 白藜蘆醇、綠膿桿菌、抑菌活性、濃度
目錄
目錄 封面內頁 簽名頁 授權書... iii 中文摘要... iv 英文摘要... v 誌謝... vi 目錄... vii 圖目錄... ix 表目
錄... xi 1. 前言... 1 2. 文獻回顧... 3 2.1 綠膿桿
菌... 3 2.2 綠膿桿菌感染方式與症狀... 4 2.3 綠膿桿菌的致病機制... 6 2.4 白藜蘆醇之特 性... 9 2.4.1 白藜蘆醇之抑菌性... 18 2.4.2 白藜蘆醇之抗氧化性... 18 2.4.3 白藜蘆醇之抗 發炎力... 19 2.4.4 白藜蘆醇之抗癌力... 19 3. 材料與方法... 20 3.1 實驗材料及方 法... 20 3.1.1 實驗材料... 20 3.1.2 儀器設備... 21 3.2 菌種培養方
法... 21 3.3 實驗方法... 23 3.4 抑菌率之計算... 24 4. 結果與討
論... 25 4.1 乙醇之抑菌性試驗... 25 4.2 DMSO 界面活性劑之抑菌試驗... 25 4.3 50% 白 藜蘆醇之抑菌試驗... 25 4.4 98% 白藜蘆醇之抑菌試驗... 34 5. 結論... 43 參考文 獻... 44
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