Inhibition of Endothelial Adhesion Molecule Expression by Monascus Metabolites

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tng-HsiangChenandChih-PeiLiΠ

GraduateInstituteUfIntegratedⅣ Iedicine、 DepaΠnlentUΓ

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IedicaIReseaIch,China

ⅣIedicaIIJniversityandIIUspital,Ta:chung‵ Tai〝an:DepartnlentUfPatllUlUgyand

LabUratUryN.Iedicine,TaipeiX/eteranSGel〕 eΓalHUspitaI‵ Taipei,Tai、van:Depaltnlent

UfBiUtechnUlUgyandLabUfatUrySciencein liedicineandlnstittlteUfBiUtechnUlUgy

inMedici【le,NatiUnalYansN｛ ingUniversity,Taipei,Taiwan

BackgrΦ uid:InflallllllatiUn isan independent㎡ skfactUrUfcafdjUvasculardiseases and assUciated

、vith endUtllelial dysilnctiUn. i↓如Γ9a∫ε2J∫ P〃KpIrΓt外

-ferlllented rice, cUntaining naturally-Uccurring statins and variUus pi:Γ nents, has Iipid-mUdulating,

anti一inflalllIllatUrlandantiUxidativeeΠ治cts

ⅣIethUdsandResⅡ 1ts:TheefrectsUfInUnacUliⅡ K,ankailavin,andlllUnascin,asthe

meItabUlites flUn〕奶〃σ“ 口r‘9-1陌Γ︳lented rice Un the expressiUn Uf cell adhesiUn

mUIecul站 (㏑tercellular adh㏄ iUn mUlecule-l/ICAM-l, vascular cell adllesiUn

mUIeculaf-VVCAM-I,andE-selectin〕

bytLlmUrnecrUsisfactUr(TNΓ

)-a-treated

humanaUlt托

elldUthel㏑

lcells(HAEcslwereinvestigated.SuppIementUfHAECs

with these 〦仍F〃芬σf6-fermented rice metabUlites signi且 cantly suPpressed cellular

bindingbetweenthehumanmUnUc” iccellsU937andTNㄗ

-a一_sti】_{llulatedHAECs‘}

IIllIllunUblUtanalvsisShU〝

一edthat叭 o〃

a“Ir∫-fermentedricemetabUlites si野 iΠcantly

attenuated 丁NF-a-induced UfVCANl-l and E-selectin but nUt ICA小 √＿I prUtein

expressiUn Gel shift assays shUwed that 蟻 UJ9rJ∫aJ∫-felIⅢented rice metabUlites

〔reatment redllcedTNΓ-a-act行_{atedtranscHption fact㏄} _{nuc.leaffactUf(NF)-KB.} FuπherIllU了e. ilrr,,,a‵ 62f∫-fenl9ented ricenletabUIites aIsU attenuated reactiveUxygen

∫pecies｛RUS)generat㏑ nj〃 ╮,′〞

vandinTNF-a-treatedHAECs.SupPlementwithan

RUS sca、

tngeΥ Λ╭-ace妙_{1-cystdne gave similar resul裕 aS CU●}llpafed witll

i五”Γa∫‘︴r∫-feⅡ工lentedricemetabUlites.

CUnC︳田S:UⅡ: 北ㄌ刀血96t公-j陌rllIented fice metabUliteS reduced TNF-a-stimulated

endUthelial adhesiveness as

、vell as dUⅥ

/n-re●_{泌lating intracellular RUS 山}fmatiUn,

NF-KB activatiUn, andVCA∫

I-l/E一selectinexpressiUn inHAECs, suppUl-tingthe

nUtiUn that the variUus nletabUIites 士Ulll Λrr,〃f,∫‘工r∫-feΠllented rice nlight have

potenjal iIIlplicatiUns in clinica︳ atherUscIefUsis disease. IJ∫ ‘jrbθˊ4gⅡt 2()1l Aug

︳∫:91(︳°〕:1951-S.j