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ABSTRACT

Therapeutic ultrasound (US) has been widely used in the treatment of

musculoskeletal injuries for decades. Despite many physiological effects have been

reported to verify the value of US in clinic, very few researches confirmed the real

mechanisms. For years, it has been well-known that heat shock proteins (HSPs) play

important roles in cellular protection by resistance to environmental stresses or enhancing the cell repair. The main purpose of this study is to investigate the

relationship of the synthesis of HSPs and ultrasound.

In this study, adult male Sprague Dawley rats were employed. For every rat,

right hindlimb was true-sonicated, while left one was mock-sonicated as control. The

muscle specimens were removed 15 hours after last sonication. The detection of HSP72

was perfonned by polyacrylamide gel electrophoresis, Westem blot, and

immunochemical stain by monoclonal anti-HSPn antibody.

The study was designed to verifY the fact that US can increase HSP72 synthesis in the skeletal muscle, and to detennine the appropriate dose of US on enhancement of

HSP72 synthesis. The content of this study is as follows.

1. Detect the levels of HSP72 in gastrocnemius muscle after sonication at different intensities of 1/2 W/cm2, 2/3 W/cm2, 5/6 W/cm2, 1 W/cm2. The muscular

temperature was also detected.

2.Compare the levels of HSP72 in gastrocnemius muscle after sonication at the appropriate intensity obtained from the above result for 5 min, 10 min, 15 min,

20 min.

3 .According to the established rat model in studying heat shock response, we

appropriate intensity obtained from the above result with that after whole­ bodily heated by means of electric beating pad.

4.In the mode-dependent study, we compared the level of HSP_n in gastrocnemius muscle after sonication with the pulse or continuous mode.

5.To observe the accumulative effect, rats were assigned into two bTfOUps to

receive 3 times and 5 times sonications respectlvely.Then the levels of HSP_n were detected.

6.The gastrocnemius muscles sonicated with the above appropriate dose were

removed after one day and seven days respectively for morphological

changes.

The results showed that 1) 2/3 W/cm2, 5/6 W/cm2, and I W/cm2 induce more

HSPn synthesis, but the effect of 112 W/cm2 in HSPn induction was unapparent. The

difference of the muscular temperature changes between the bTfOUps receiving 1/2

W/cm2 and 2/3 W /cm2 treatment respectively was significant 2) In the duration­ dependent study, we found that the amount ofHSPn synthesis significantly increased in

the group of receiving 20 min sonication .. 3) US induced more HSP_n synthesis as the electric heating pad does. 4) The rats which were sonicated in the continuous mode

enhanced apparently HSP_n synthesis. 5) the rats which were sonicated daily for 5 days can induce more HSPn synthesis. Finally, 6) Light microscopy demonstrated no

necrosis or inflammatoI)' conditions between mock-and true-sonicated muscles.

In conclusion, application of 1 MHz therapeutic ultrasound at the average

intensity of 213 W/cm2 for 20 minutes in the continuous mode is an appropriate dose in enhancing HSP_n synthesis. The mechanism of HSP_n induction with US is due to

thennal effect. The dose-dependent response and accumulative effect were present.

Apparently, HSPn is an index on the thennal effects of US. The rat model is a reliable

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,

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I ., 1976)

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1994) , ;fl~91tj, rfiJn'itt~tr:l lZQ(t':]ljCj

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1991:

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&

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1992) 0

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(fibroblasts)

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1988) 0

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1988) ,

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1989)

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1994)

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Japan

b.

~~~:

Model S 707, MINK Co., Taiwan

c. $lffJCA~: (J)

Sorvall RT 6000D, Feng Jih Biomedical

&

Instruments

Co., LTD., Taiwan, R.O.C.

(2)

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d.

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Model 357544, Wheaton

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2000, Japan

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gland

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Germany

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' - L

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