Author(s): Chen, CY (Chen, Chi-Yuan); Chiou, SH (Chiou, Shih-Hwa); Huang, CY (Huang, Chih-Yang); Jan, CI (Jan, Chia-Ing); Lin, SC (Lin, Shu-Chun); Tsai, ML (Tsai, Ming-Long); Lo, JF (Lo, Jeng-Fan)
Title: Distinct population of highly malignant cells in a head and neck squamous cell carcinoma cell line established by xenograft model
Source: JOURNAL OF BIOMEDICAL SCIENCE, 16: Art. No. 100 NOV 16 2009 Language: English
Document Type: Article
KeyWords Plus: CANCER STEM-CELLS; BMI-1 ONCOPROTEIN; BREAST-CANCER;
SELF-RENEWAL; PROSPECTIVE IDENTIFICATION; EPITHELIAL-CELLS; TUMOR- SUPPRESSOR; EXPRESSION; PATHWAY; SUBPOPULATION
Abstract: The progression and metastasis of solid tumors, including head and neck squamous cell carcinoma (HNSCC), have been related to the behavior of a small
subpopulation of cancer stem cells. Here, we have established a highly malignant HNSCC cell line, SASVO3, from primary tumors using three sequential rounds of xenotransplantation.
SASVO3 possesses enhanced tumorigenic ability both in vitro and in vivo. Moreover, SASVO3 exhibits properties of cancer stem cells, including that increased the abilities of sphere-
forming, the number of side population cells, the potential of transplanted tumor growth and elevated expression of the stem cell marker Bmi1. Injection of SASVO3 into the tail vein of nude mice resulted in lung metastases. These results are consistent with the postulate that the malignant and/or metastasis potential of HNSCC cells may reside in a stem-like
subpopulation.
Addresses: [Chen, Chi-Yuan; Lin, Shu-Chun; Lo, Jeng-Fan] Natl Yang Ming Univ, Inst Oral Biol, Taipei 112, Taiwan; [Chiou, Shih-Hwa; Tsai, Ming-Long] Taipei Vet Gen Hosp, Dept Med Res & Educ, Taipei, Taiwan; [Huang, Chih-Yang] China Med Univ, Grad Inst Chinese Med Sci, Taichung, Taiwan; [Huang, Chih-Yang] China Med Univ, Inst Med Sci, Taichung, Taiwan;
[Huang, Chih-Yang] China Med Univ, Inst Basic Med Sci, Taichung, Taiwan; [Huang, Chih- Yang] Asia Univ, Dept Hlth & Nutr Biotechnol, Taichung, Taiwan; [Jan, Chia-Ing] China Med Univ & Hosp, Dept Pathol, Taichung, Taiwan
Reprint Address: Lo, JF, Natl Yang Ming Univ, Inst Oral Biol, Taipei 112, Taiwan.
E-mail Address: [email protected]; [email protected];
[email protected]; [email protected]; [email protected];
[email protected]; [email protected] Funding Acknowledgement:
Funding Agency Grant Number
National Science Council NSC95N444
NSC97N456
Taipei Veterans General Hospital
V95E2007 V95B2013 V96ER2016 V97ER2018 V98ER2018
Ministry of Education
96ADD122 97ACD113 97ACT302 98ACT302
This study was supported by research grants from National Science Council (NSC95N444 and NSC97N456), Taipei Veterans General Hospital (V95E2007, V95B2013, V96ER2016, V97ER2018 and V98ER2018) and Ministry of Education (96ADD122, 97ACD113, 97ACT302 and 98ACT302) in Taiwan, ROC.
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Publisher: BIOMED CENTRAL LTD
Publisher Address: 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND ISSN: 1021-7770
Article Number: 100
DOI: 10.1186/1423-0127-16-100
29-char Source Abbrev.: J BIOMED SCI ISO Source Abbrev.: J. Biomed. Sci.
Source Item Page Count: 11
Subject Category: Medicine, Research & Experimental ISI Document Delivery No.: 525PS
