國立成功大學「邁向頂尖大學計畫」
延攬優秀人才工作報告表
NCKU’s “Aim for the Top University Project”
Work Report Form for Distinguished Scholars
□續聘continuation of employment █離職resignation
100 年 7 月 13 日更新
受聘者姓名 Name of the Employee
Periasamy
Srinivasan 男 女Male Female
聘 期 Period of Employment
from 年(y) 2009 月(m) 6 日(d) 1 to 年(y) 2013 月(m) 7 日(d) 26 研究或教學或科技研發與
管理計畫名稱 The project title of research,
teaching, technology development and management
探討多種重金屬與感染的交互 作用對環境衛生安全的重要性
計畫主持人
（申請單位主管）
Project Investigator (Head of Department/Center)
劉明毅
補助延聘編號
Grant Number HUA100-3-7-119
一、研究、教學、科技研發與管理工作全程經過概述。（由受聘人填寫）
Please summarize the entire research, teaching, or science and technology R&D and management work process (To be completed by the employee)
Comparative potential therapeutic effect of sesame oil and peanut oil against acute monocrotaline (Crotalaria) poisoning in a rat model.
Many Crotalaria plant species contain hepatotoxic pyrrolizidine alkaloids (such as monocrotaline) that can cause acute and chronic poisoning in cattle and other animals. Peanut oil, atropine sulfate, and antidiarrheal agents are used to treat acute monocrotaline poisoning. The effect of sesame on acute monocrotaline poisoning has never been investigated. Monocrotaline significantly decreased (P < .05) serum amylase activity, but, over time, increased (P < .05) pancreatic and lung injury. AST and ALT activity and liver injury peaked at 24 hours. Sesame oil and peanut oil (P < .05) inhibited the changes in all tested parameters in acute monocrotaline poisoning. Although peanut oil inhibited acute monocrotaline poisoning, it induced steatosis, but sesame oil did not. We hypothesize that early pancreatic and lung injury and late liver injury contribute to acute monocrotaline poisoning and that sesame oil is more efficacious than peanut oil against acute monocrotaline poisoning in rats. However, additional studies are needed to confirm that these oils have the same effects in cattle and other animals.
Effect of sesamol and sesame oil on monocrotaline induced-sinusoidal obstruction syndrome in rats (Protective and therapeutic role).
Sinusoidal obstruction syndrome (SOS) occurs in patients undergoing hematopoietic cell transplantation and chemotherapy. The chemotherapeutic drugs oxaliplatin and cyclophosphamide cause SOS. Male Sprague-Dawley rats were gavaged with a single dose of sesame oil (0.5, 1, 2, or 4 mL/kg) One hour later, those rats were gavaged with monocrotaline (90 mg/kg) to induce SOS. and sesamol (5, 10, 20, and 40 mg/kg) was subcutaneously injected 24 h after monocrotaline treatment. Aspartate transaminase, alanine transaminase, laminin, collagen, myeloperoxidase, nitrate content, lipid peroxidation,
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glutathione levels, matrix metalloproteinase (MMP)-9, and tissue inhibitor of matrix metalloproteinases (TIMP)-1 were assessed.
Effect of sesamol on isoproterenol-induced acute myocardial infarction in rats.
The protective role of sesamol and its possible action against isoproterenol-induced myocardial injury and infarction is unknown. We tested the hypothesis that sesamol's protection against myocardial infarction is associated with the inhibition of matrix metalloproteinase (MMP)-2 and MMP-9.Four groups of experimental rats were subcutaneously injected with sesamol (0, 1, 3, or 10 mg/kg) and then, 2 h later, intraperitoneally injected with isoproterenol (100 mg/kg 24 h apart on 2 consecutive days) to induce myocardial infarction. Blood pressure (BP), heart rate (HR), and electrocardiography (ECG) wave durations, serum creatine phosphokinase isoenzymes (CKMB), lactate dehydrogenase (LDH), myocardial histology, MMP-2, and MMP-9 were assessed 24 h after the last dose of isoproterenol was given.
Ostensibly ineffectual doses of cadmium and lipopolysaccharide causes liver damage in rats.
We found that co-exposing rats to ostensibly ineffectual doses of Cd and LPS significantly increased their AST, ALT, NO, peroxynitrite, and LPO levels but decreased their MT level. In addition, they underwent marked histopathological damage. The present study, however, clearly shows that even ostensibly ineffectual doses of Cd plus LPS injure rats’ livers. LPS initiated liver injury by increasing IL-1b levels and decreasing MT levels. Cd synergistically acted with LPS by increasing NO,
peroxynitrite, and LPO levels, but decreasing MT levels, which caused oxidative stress and liver damage.
In addition to proposed project, I was also involved in the following studies.
Effect of sesame oil on trinitrobenzene sulfonic acid induced Crohn’s disease in rats.
I have established Crohn’s disease model using trinitrobenzene sulfonic acid (TNBS) in rats and treated with sesame oil for 7 days. After 7-day treatment of sesame oil showed significant decrease in the weight/length, thickness, extension of lesion in colon, macroscopic, microscopic score and diahorrea. Preliminary data shows promising inhibitory role of sesame oil on Crohn’s disease.
Further studies are in progress.
Histopathological, histochemical and immunohistochemical staining
Apart from the other studies in progress, I have also done some considerable work in histochemical analysis in various disease and toxicology animal models. Extensive histopathological work was done in gentamicin induced renal damage and its recovery by sesame oil, which includes masson
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trichrome, periodic acid schiffs, mast cell and AgNOR staining, in addition we have also done immunohistochemical staining for laminin in Crohn’s disease and the role of sesame oil.
Publications
Hsu DZ, Chen YW, Chu PY, Periasamy S, Liu MY. Protective effect of 3,4-
methylenedioxyphenol (sesamol) on stress-related mucosal disease in rats. Biomed Res Int.
2013; 2013:481827.
Liu CT, Periasamy S, Chang CC, Mo FE, Liu MY. Sesame Oil Therapeutically Ameliorates Cardiac Hypertrophy by Regulating Hypokalemia in Hypertensive Rats. JPEN J Parenter Enteral Nutr. 2013 Jun 10.
Hsu DZ, Liu CT, Chu PY, Li YH, Periasamy S, Liu MY. Sesame oil attenuates ovalbumin- induced pulmonary edema and bronchial neutrophilic inflammation in mice. Biomed Res Int.
2013; 2013:905670.
Periasamy S, Hsu DZ, Chandrasekaran VR, Liu MY. Sesame oil accelerates healing of 2,4,6- trinitrobenzenesulfonic Acid-induced acute colitis by attenuating inflammation and fibrosis.
JPEN J Parenter Enteral Nutr. 2013; 37(5):674-82.
Srinivasan P, Liu MY. Comparative potential therapeutic effect of sesame oil and peanut oil against acute monocrotaline (Crotalaria) poisoning in a rat model. J Vet Intern Med. 2012;
26(3):491-9.
Pandurangan AK, Periasamy S, Anandasadagopan SK, Ganapasam S, Srinivasalu SD. Green tea polyphenol protection against 4-nitroquinoline 1-oxide-induced bone marrow lipid peroxidation and genotoxicity in Wistar rats. Asian Pac J Cancer Prev. 2012; 13(8):4107-12.
Periasamy S, Liu MY. Sinusoidal injury induction: monocrotaline dose and hepatic sinusoidal injury in rats not correlated. J Surg Oncol. 2013; 107(4):447.
Periasamy S, Yang SS, Chen SY, Chang CC, Liu MY. Prophylactic sesame oil attenuates sinusoidal obstruction syndrome by inhibiting matrix metalloproteinase-9 and oxidative stress.
JPEN J Parenter Enteral Nutr. 2013; 37(4):529-37.
Periasamy S, Chien SP, Liu MY. Therapeutic oral sesame oil is ineffectual against
monocrotaline-induced sinusoidal obstruction syndrome in rats. JPEN J Parenter Enteral Nutr.
2013; 37(1):129-33.
Periasamy S, Hsu DZ, Chen SY, Yang SS, Chandrasekaran VR, Liu MY. Therapeutic sesamol attenuates monocrotaline-induced sinusoidal obstruction syndrome in rats by inhibiting matrix metalloproteinase-9. Cell Biochem Biophys. 2011; 61(2):327-36.
Periasamy S, Mo FE, Chen SY, Chang CC, Liu MY. Sesamol attenuates isoproterenol-induced acute myocardial infarction via inhibition of matrix metalloproteinase-2 and -9 expression in rats. Cell Physiol Biochem. 2011; 27(3-4):273-80.
Hsu DZ, Li YH, Chu PY, Periasamy S, Liu MY. Sesame oil prevents acute kidney injury
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induced by the synergistic action of aminoglycoside and iodinated contrast in rats. Antimicrob Agents Chemother. 2011; 55(6):2532-6.
Periasamy S, Chen SY, Hsu DZ, Liu MY. Comments on Vennila and Pugalendi. Vennila L, Pugalendi KV. Protective effect of sesamol against myocardial infarction caused by
isoproterenol in Wistar rats. Redox Rep 2010; 15: 36-42. Redox Rep. 2010; 15(6):288-9.
Raghavendran HR, Srinivasan P, Rekha S. Immunomodulatory activity of fucoidan against aspirin-induced gastric mucosal damage in rats. Int Immunopharmacol. 2011; 11(2):157-63.
Periasamy S, Chen SY, Liu MY. The study of ISO induced heart failure rat model, Exp Mol Pathol. 2010; 88:299-304. Exp Mol Pathol. 2011; 90(1):84.
Chandrasekaran VR, Periasamy S, Liu LL, Liu MY. 17β-Estradiol protects against
acetaminophen-overdose-induced acute oxidative hepatic damage and increases the survival rate in mice. Steroids. 2011; 76(1-2):118-24.
Periasamy S, Liu CT, Hsu DZ, Liu MY. Sesame oil accelerates kidney healing following gentamicin-induced kidney injury in rats. Am J Nephrol. 2010; 32(5):383-92.
Srinivasan P, Li YH, Hsu DZ, Su SB, Liu MY. Ostensibly ineffectual doses of cadmium and lipopolysaccharide causes liver damage in rats. Hum Exp Toxicol. 2011; 30(7):624-35.
二、研究或教學或科技研發與管理成效評估（由計畫主持人或單位主管填寫）
Please evaluate the performance of research, teaching or science and technology R&D and management Work: ( To be completed by Project Investigator or Head of Department/Center)
(1)是否達到延攬預期目標？
Has the expected goal of recruitment been achieved? – Yes, the expected goal of recruitment has been achieved.
(2)研究或教學或科技研發與管理的方法、專業知識及進度如何？
What are the methods, professional knowledge, and progress of the research, teaching, or R&D and management work?-As shown in the report.
(3)受延攬人之研究或教學或科技研發與管理成果對該計畫(或貴單位)助益如何？
How have the research, teaching, or R&D and management results of the employed person given benefit to the project (or your unit)?- The employed person benefited to the project and he has published papers.
(4)受延攬人於補助期間對貴單位或國內相關學術科技領域助益如何？
How has the employed person, during his or her term of employment, benefited your unit or the relevant domestic academic field?- The employed person benefited my unit and the relevant academic fields.
(5)具體工作績效或研究或教學或科技研發與管理成果：
Please describe the specific work performance, or the results of research, teaching, or R&D and management work:
Specific work performance, the results of research have been described above in the work report.
(6)是否續聘受聘人? Will you continue hiring the employed person? █續聘Yes □不續聘No
※此報告表篇幅以三～四頁為原則。This report form should be limited to 3-4 pages in principle.
※此表格可上延攬優秀人才成果報告繳交說明網頁下載。
This report form can be downloaded in http://scholar.lib.ncku.edu.tw/explain/
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