牛樟芝保護大鼠肝臟避免酒精餵食引發傷害之研究
Protective effect of Antrodia camphorata on liver from injury of alcohol feeding in rat
中文摘要
許多研究顯示，慢性飲酒會導致血液中的 adiponectin 蛋白質降低，進而影響肝 細胞的脂質代謝，導致脂肪肝的形成與肝臟的纖維化病變。關於急性飲酒是否對 肝臟與血中 adiponectin 濃度造成影響，則尚未有研究報導。另一方面，研究報 導傳統中藥樟芝具有保護肝臟、抗氧化與抗發炎等功能；對於急性飲酒，樟芝是 否具有快速有效降低血中酒精濃度等解酒的作用，對血液中 adiponectin 濃度又 是否有影響，也是值得探討的問題。
因此本研究以大鼠為材料，實驗分成三組，樟芝(A)組(1.0 g/kg)，酒精(E)組(5.5 g/kg)，以及樟芝與酒精的混合液(A+E)組。分別在餵食後不同時間(0 分鐘、30 分鐘、60 分鐘、120 分鐘、240 分鐘與 360 分鐘)進行採血，利用酵素免疫分析試 劑(ELISA kits)測量血中酒精以及 adiponectin 濃度。結果顯示：A 組血中酒精濃 度在不同時間點與尚未餵食的 0 分鐘比較，皆沒有顯著變化。E 組血中酒精濃度 會逐漸上升，在 120 分鐘左右達到最高值(185.6 ± 31.4 mg/dL)，而後逐漸下降，
在 360 分鐘後血中酒精濃度與 0 分鐘的基準值比較沒有統計差異。另外，A+E 組對血中酒精濃度上升的趨勢與 E 組並沒有明顯不同。所以結果顯示，餵食樟 芝無法有效降低急性酒精餵食後上升的血中酒精濃度。
另一方面，E 組在 120 分鐘內並不會改變血液中 adiponectin 的濃度；但是在 240 分鐘與 360 分鐘之後，血液中 adiponectin 的濃度分別約為 0 分鐘的 0.94 倍與 0.95 倍，有些微下降的現象，但是不具統計意義。A 組對血液中 adiponectin 濃度沒 有顯著影響。A+E 組血液中 adiponectin 濃度在不同時間點也都沒有上升的情形。
綜合以上結果顯示，急性飲酒後，服用樟芝並不影響血液中酒精與 adiponectin 的濃度。因此樟芝的解酒與保肝作用，推測可能是透過保護肝臟細胞膜上的 adiponectin receptor 2，避免酒精的傷害。目前正積極分析急性飲酒以及餵食樟芝 後是否影響肝臟細胞的 adiponectin receptor 2 含量。期待能更進一步了解樟芝保 護肝細胞的機轉。
英文摘要
Several studies found that chronic ethanol intake decreased circulating adiponectin level, disrupted lipid metabolism in hepatocytes, and caused excessive lipid
accumulation with the development of fatty liver and hepatic steatosis. However, the effects of acute ethanol feeding on circulating adiponectin level and liver have not been examined. Besides, Antrodia camphorate, a traditional Chinese medicine, was found to exert hepatoprotective, anti-oxidative and anti-inflammation effects.
In this study, we examined the role of Antrodia camphorata in circulating ethanol and adponectin levels upon acute ethanol feeding in rats. Rats were divided into three groups: one group was Antrodia camphorata (1.0 g/kg) feeding, another group was ethanol (5.5g/kg) feeding, and the other group was fed with the mixture of Antrodia camphorata and alcohol. Blood sample was collected and assayed by ELISA kits at indicated time points. The results showed that Antrodia camphorata feeding did not affect the circulating ethanol levels. After acute alcohol intake, the serum ethanol levels were significantly increased to the maximum value at 120 minutes (185.6 ± 31.4 mg/dL) and then decreased to the basal level (94.2 ± 23.6 mg/dL) at 360 minutes in ethanol-fed rats. Furthermore, the circulating ethanol levels in mixture-fed rats were similar to those in ethanol-fed rats at each indicated time points. Therefore, Antrodia camphorata administration did not attenuate acute ethanol feeding-induced elevation of serum ethanol levels.
On the other hand, there was no effect of alcohol feeding on serum adiponectin levels during 120 minutes. Adiponectin levels were increased to 1.16-fold and 1.15-fold at 240 minutes and 360 minutes, respectively. However, there was no significant difference compared with 0 minute group. Given the mixture of Antrodia camphorata and alcohol or Antrodia camphorata alone did not significantly affect adiponectin serum level.
These results suggested that Antrodia camphorata intake did not suppress serum ethanol or adiponectin levels in ethanol-fed rats. We proposed that the
hepatoprotective effect of Antrodia camphorata might be due to protect the adiponectin receptor 2 (Adipo R2) on cell membrane of hepatocyte from ethanol injury. It is important to examine the Adipo R2 content in ethanol-fed and in Antrodia camphorate-fed groups. Furthermore, the mechanism of hepatoprotective effect of Antrodia camphorate could be explored.
