Author(s): Yu, CS (Yu, Chun-Shu); Lai, KC (Lai, Kuang-Chi); Yang, JS (Yang, Jai-Sing);
Chiang, JH (Chiang, Jo-Hua); Lu, CC (Lu, Chi-Cheng); Wu, CL (Wu, Chang-Lin); Lin, JP (Lin, Jing-Pin); Liao, CL (Liao, Ching-Lung); Tang, NY (Tang, Nou-Ying); Wood, WG (Wood, W.
Gibson); Chung, JG (Chung, Jing-Gung)
Title: Quercetin Inhibited Murine Leukemia WEHI-3 Cells In Vivo and Promoted Immune Response
Source: PHYTOTHERAPY RESEARCH, 24 (2): 163-168 FEB 2010 Language: English
Document Type: Article
Author Keywords: quercetin; WEHI-3 cells; BALB/c mice; in vivo; phagocytosis KeyWords Plus: INDUCED GROWTH-INHIBITION; COLON-CANCER CELLS;
CARCINOMA-CELLS; TYROSINE KINASE; FACTOR RECEPTOR; HL-60 CELLS;
FLAVONOIDS; APOPTOSIS; EXPRESSION; METASTASIS
Abstract: Enhanced flavonoid consumption is closely related with a reduced cancer incidence as shown in epidemiological studies. Quercetin (3,5,7,3',4'-pentahydroxylflavone) is one of the active components of flavonoids which exist in natural plants, particularly in onions and fruits.
It was reported that quercetin induced apoptosis in human cancer cell lines, including human leukemia HL-60 cells, but there is no available information as to its effects on leukemia cells in vivo. The purpose of the present studies was to focus on the in vivo effects or quercetin on leukemia WEHI-3 cells. The effects of quercetin on WEHI-3 cells injected into BALB/c mice were examined. Quercetin decreased the percentage of Mac-3 and CD11b markers,
suggesting that the differentiation of the precursors of macrophages and T cells was inhibited.
There was no effect on CD3 levels but increased CD19 levels. Quercetin decreased the weight of the spleen and liver compared with the olive oil treated animals. Quercetin stimulated macrophage phagocytosis or cells isolated from peritoneum. Quercetin also promoted natural killer cell activity. Based on pathological examination, an effect of quercetin was observed in the spleen of mice previously injected with WEHI-3 cells. Apparently, quercetin affects WEHI-3 cells in vivo. Copyright (C) 2009 John Wiley & Sons, Ltd.
Addresses: [Wu, Chang-Lin; Chung, Jing-Gung] China Med Univ, Dept Biol Sci & Technol, Taichung 404, Taiwan; [Yu, Chun-Shu] China Med Univ, Ctr Gen Educ, Taichung 404, Taiwan; [Lai, Kuang-Chi] China Med Univ, Beigang Hosp, Dept Surg, Beigang 651, Yunlin, Taiwan; [Lai, Kuang-Chi] China Med Univ, Sch Med, Taichung 404, Taiwan; [Yang, Jai-Sing]
China Med Univ, Dept Pharmacol, Taichung 404, Taiwan; [Chiang, Jo-Hua; Lu, Chi-Cheng]
Natl Chung Hsing Univ, Dept Life Sci, Taichung 402, Taiwan; [Lin, Jing-Pin; Tang, Nou-Ying]
China Med Univ, Sch Chinese Med, Taichung 404, Taiwan; [Liao, Ching-Lung] China Med Univ, Grad Inst Chinese Med Sci, Taichung 404, Taiwan; [Wood, W. Gibson] Univ Minnesota, Sch Med, Dept Pharmacol, Ctr Geriatr Res Educ & Clin,VA Med Ctr, Minneapolis, MN 55455
USA; [Chung, Jing-Gung] Asia Univ, Dept Biotechnol, Taichung 413, Taiwan
Reprint Address: Chung, JG, China Med Univ, Dept Biol Sci & Technol, 91 Hsueh Shih Rd, Taichung 404, Taiwan.
E-mail Address: [email protected] Funding Acknowledgement:
Funding Agency Grant Number
China Medical University, Taichung, Taiwan CMU95-330
This work was supported by Grant CMU95-330 from China Medical University, Taichung, Taiwan.
Cited References: CHEN TJ, 2004, TOXICOL LETT, V147, P1, DOI 10.1016/j.toxlet.2003.10.012.
GRANADOSERRANO AB, 2006, J NUTR, V136, P2715.
HE Q, 2001, LEUKEMIA RES, V25, P455.
HIBASAMI H, 2005, INT J MOL MED, V15, P805.
HUANG YT, 1999, BRIT J PHARMACOL, V128, P999.
KANDASWAMI C, 2005, IN VIVO, V19, P895.
LEE LT, 2004, BIOCHEM PHARMACOL, V67, P2103, DOI 10.1016/j.bcp.2004.02.023.
LEE TJ, 2006, CANCER LETT, V240, P234, DOI 10.1016/j.canlet.2005.09.013.
LIN HY, 2003, BIOCHEM PHARMACOL, V66, P1821, DOI 10.1016/S0006-2952(03)00422-2.
LOKEN MR, 2000, FLOW CYTOMETRY PRACT, P61.
MERTENSTALCOTT SU, 2003, J NUTR, V133, P2669.
MUTOH H, 2000, JPN J CANCER RES, V91, P686.
NADERI GA, 2003, MOL CELL BIOCHEM, V246, P193.
NGUYEN TTT, 2004, CARCINOGENESIS, V25, P647, DOI 10.1093/carcin/bgh052.
ONG CS, 2004, ONCOL REP, V11, P727.
PIANTELLI M, 2006, J CELL PHYSIOL, V207, P23, DOI 10.1002/jcp.20510.
SHEN SC, 2003, J CELL BIOCHEM, V89, P1044, DOI 10.1002/jch.10559.
SINGHAL RL, 1995, BIOCHEM BIOPH RES CO, V208, P425.
STACHOWSKA E, 2007, LIPIDS, V42, P707, DOI 10.1007/s11745-007-3072-2.
SU CC, 2008, IN VIVO, V22, P63.
TAN WF, 2003, EUR J PHARMACOL, V459, P255, DOI 10.1016/S0014-2999(02)02848-0.
VANERK MJ, 2005, EUR J NUTR, V44, P143, DOI 10.1007/s00394-004-0503-1.
VIJAYABABU MR, 2006, MOL CELL BIOCHEM, V287, P109, DOI 10.1007/s11010-005-9085- 3.
WENZEL U, 2000, CANCER RES, V60, P3823.
YANG JS, 2006, ANTICANCER RES, V26, P219.
YU FS, 2006, IN VIVO, V20, P147.
ZHANG X, 2000, CLIN EXP METASTAS, V18, P415.
ZHANG XM, 2004, CANCER CHEMOTH PHARM, V53, P82, DOI 10.1007/s00280-003-0702- 0.
Cited Reference Count: 28 Times Cited: 0
Publisher: JOHN WILEY & SONS LTD
Publisher Address: THE ATRIUM, SOUTHERN GATE, CHICHESTER PO19 8SQ, W SUSSEX, ENGLAND
ISSN: 0951-418X DOI: 10.1002/ptr.2841
29-char Source Abbrev.: PHYTOTHER RES ISO Source Abbrev.: Phytother. Res.
Source Item Page Count: 6
Subject Category: Chemistry, Medicinal; Pharmacology & Pharmacy ISI Document Delivery No.: 558WD
