Author(s): Lo, SF (Lo, S. -F.); Huang, CM (Huang, C. -M.); Lin, HC (Lin, H. -C.); Chen, WC (Chen, W. -C.); Tsai, CH (Tsai, C. -H.); Tsai, FJ (Tsai, F. -J.)
Title: Cytokine (IL-6) and chemokine (IL-8) gene polymorphisms among rheumatoid arthritis patients in Taiwan
Source: CLINICAL AND EXPERIMENTAL RHEUMATOLOGY, 26 (4): 632-637 JUL-AUG 2008
Language: English Document Type: Article
Author Keywords: interleukin-6; interleukin-8; gene polymorphism; rheumatoid arthritis KeyWords Plus: INTERLEUKIN-8 MESSENGER-RNA; PROMOTER POLYMORPHISMS;
SYNOVIAL FIBROBLASTS; EXPRESSION; ASSOCIATION; PROTEIN; STABILIZATION;
TRANSCRIPTION; SYNOVIOCYTES; ACTIVATION Abstract: Objective
The involvement of cytokines and chemokines in the pathogenesis of rheumatoid arthritis (RA) is well studied: however, the genetic bases behind this is not well understood. The aim of this study was to examine whether -572 G/C polymorphism in the IL-6 gene and 2767 A/G polymorphism in the 3-untranslated region (UTR) of the IL-8 gene are associated with rheumatoid arthritis (RA).
Methods
We enrolled 199 RA patients and 130 normal controls. Polymerase chain reaction was used to identify the IL-6-572G/C and IL-8 3-UTR 2767A/G polymorphisms. The relationships between clinical manifestations of RA and the polymorphisms of each gene were investigated by comparing the genotypes among RA patients with different clinical variables.
Results
We found no significant difference in the genotypic and allelic frequencies of the single nucleotide polymorphisms of IL-6 and IL-8 genes between RA patients and controls. Clinical characteristics such as age at onset, rheumatoid factor positivity, joint erosion and extra- articular manifestations were compared among patients with different genotypes of the IL-6 and IL-8 genes. We found that patients with IL-8 3-UTR 2767AA genotype had a significantly younger age of onset of RA than patients without that genotype.
Conclusion
The IL-6 -572 G/C and IL-8 3-UTR 2767A/G polymorphisms are not associated with the risk of developing rheumatoid arthritis. However, the finding that patients with IL-8 3-UTR 2767AA developed RA at a younger age suggests that this genotype may influence the etiopathology of RA in patients in Taiwan. Therefore, further single nucleotide polymorphism studies of this 3 UTR region may give more novel findings and understanding of the genetic basis of
rheumatoid arthritis.
Addresses: [Tsai, F. -J.] China Med Univ Hosp, Dept Med Genet, Taichung, Taiwan; [Lo, S.
-F.] China Med Univ Hosp, Dept Phys Med & Rehabil, Taichung, Taiwan; [Huang, C. -M.]
China Med Univ Hosp, Dept Internal Med, Div Rheumatol & Immunol, Taichung, Taiwan;
[Chen, W. -C.] China Med Univ Hosp, Dept Urol, Taichung, Taiwan; [Tsai, C. -H.; Tsai, F. -J.]
China Med Univ Hosp, Dept Pediat, Taichung, Taiwan; [Lin, H. -C.] China Med Univ, Coll Hlth Care, Dept Phys Therapy, Taichung, Taiwan; [Huang, C. -M.; Chen, W. -C.] China Med Univ, Grad Inst Integrated Med, Taichung, Taiwan; China Med Univ, Coll Chinese Med, Sch Post Baccalaureate Chinese Med, Taichung, Taiwan; [Tsai, C. -H.] Asia Univ, Grad Inst
Bioinformat, Taichung, Taiwan
Reprint Address: Tsai, FJ, China Med Univ Hosp, Dept Med Genet, 2 Yuh Der Rd, Taichung, Taiwan.
E-mail Address: [email protected]
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Publisher: CLINICAL & EXPER RHEUMATOLOGY
Publisher Address: VIA SANTA MARIA 31, 56126 PISA, ITALY ISSN: 0392-856X
29-char Source Abbrev.: CLIN EXP RHEUMATOL ISO Source Abbrev.: Clin. Exp. Rheumatol.
Source Item Page Count: 6 Subject Category: Rheumatology ISI Document Delivery No.: 349IX
