以下列出
control（CBe）及黃柏（BBe）、黃連（LBe）
、黃芩（TBe）的 house-keeping gene GAPDH 以及 Ubiquitin C 的原始讀數及校正後的數值.Control 組對 1107CBe校正
黃柏、黃連、黃芩對 1107CBe校正後
ArrayAD HsID Title 1107BBe bb 3rd 1118 BB 1107BBe" bb 3rd" 1118BB"
<60,084> NA GAPDH 36384 38417 48687 33957.89 26581.64 35718.91
1107LBe lb 3rd 1118 LB
1107LBe" lb 3rd" 1118LB"36811 27983 54249
31953.38 16616.08 37008.321107TBe tb 2nd 1118TB2
1107TBe" tb 2nd" 1118TB2"0 0 230
3074.90 2328.60 1797.43<66,037> Hs.183704 Ubiquitin C 1107BBe bb 3rd 1118 BB 1107BBe" bb 3rd" 1118BB"
42238 40004 57434 39357.62 27757.76 41875.05
1107LBe lb 3rd 1118 LB
1107LBe" lb 3rd" 1118LB"46766 39543 65535
40557.48 24587.85 44174.931107TBe tb 2nd 1118TB2
1107TBe" tb 2nd" 1118TB2"44466 50242 51256
32355.76 32699.89 31264.94ArrayAD HsID Title 1107CBe cb 3rd 1118 CB
cb 3rd" 1118 CB"<60,084> NA GAPDH 29418 26708 45430 29170.28 34719.82
<66,037> Hs.183704 Ubiquitin C 41723 35309 55540 38567.73 42300.30
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５１
ABSTRACT
Traditional Chinese medicine ( TCM ) has been extensively used to treat many diseases in eastern society throughout thousands of years. However, the diagnostic and therapeutic concepts were not identical to those of modern western medicine. The major principles of TCM are treating patients based on the clinical disease patterns. For example, hot disease patterns could be treated with so-called cold medicines and they have the ability to relieve internal hotness of the human body, decrease metabolic rate,and sympathetic tone, etc.
In the past few years, a new technology, DNA microarray ( DNA chip ), has made it possible to simultaneously study the expression of thousands genes in a single chip.
In this pilot study, we try to elucidate the gene expression of the HEK 293 cell in vitro under the treatment by 3 traditional Chinese medicine (Scutellaria baicalensis Georgi 、 Coptis chinensis Franch. and Phellodendron Chinese Schneid.), which belongs to the “cold” medicine. For this, gene expression of herbs treated cells and none treated cells was compared simultaneously using Dr. Peck Konan microarray system. Dual color staining method of enzyme colorimetry detection system was utilized by biotin-16-dUTP during reverse transcription. A color microarray image was rendered by the chromogens yielded by the enzymatic reactions.
The result showed changes in expressions of 21 genes up regulation and 386 genes down regulation by Scutellaria baicalensis and; 17 genes up regulation and 112 genes down regulation by Coptis chinensis and ; 103 genes up regulation and 108 genes down regulation by Phellodendron.
This study established a method to the understanding of the genetic influence of TCM(Scutellaria baicalensis Georgi 、 Coptis chinensis Franch. and Phellodendron Chinese Schneid.). Further studies are needed to verify these results.