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saline( 5ul ) 或
saline( 5ul ) CGS(1ug/5ul) 或 或 埋管 5ul saline 5ul saline 5ul saline NPY(10ug/5ul) CPA(10ug/5ul)
fasting 1hr 2hr 3hr. 4hr
day 1 day2 day 3 day4 day 5 5 分鐘後 記錄攝食量
圖一、腦室內注射神經胜? Y 對腺核? A1(CPA)或 A2a(CGS)接受器致效劑引起降低大白鼠攝食行為作用的影 響之流程圖。 攝食量紀錄時間。
day1 day5 1hr. 2hr. 3hr. 4hr. day6 day7 day8 day9 重複
埋管 ZM 5 分鐘後 紀錄攝食量 間隔 48 小時 ZM 或 或 DPCPX DPCPX
圖二、腦室內注射 1、5、10μg 腺核? A1(DPCPX)或 A2a(ZM)接受器拮抗劑對大白鼠食物攝取的影響之流程圖。
攝食量紀錄時間。
0
cunulated 1hr food intake( g )
saline+saline
cumulated 4h food intake ( g )
##
saline ; *p<0.01;ns(not significant)。上圖為累積 1h 之攝 食量;下圖為累積 4h 之攝食量。
0
cumulated 1hr food intake ( g )
saline+saline
saline ; *p<0.01;ns(not significant)。上圖為累積 1h 之攝
0 5 10 15 20 25 30
1 2 3 4
NPY level ( % area )
ARC PVN
saline CPA 1ug CPA 5ug CPA 10ug
圖五、腺核? 對神經胜 @Y 表現量的調節作用(n=6-10)。
由腦室內注射 1、5、10μg CPA 或 saline 後,對弓形核
(ARC)、室旁核(PVN)之神經胜 @Y 含量之影響。各
0 10 20 30 40 50
1 2 3 4
NPY level ( % area )