1 前言
4.1 懸浮式系統對不同染料之脫色比較
4.3.3 含高濃度芳香胺中間物條件下對 A. hydrophila NIU01PHA 產量
具有高度生物毒性，因此在 MR 培養基質這類貧脊的環境中菌體無法取得 足夠能量抵抗中間物所造成之生物毒性，導致無法生長而致使結果無法測 量，而 3AP 雖未具高度生物毒性，卻也造成生長壓力，所產生之乾菌重及
PHA 含量無論何種培養基質皆較低，但仍以氮源缺乏時所產生之 PHA 含量 較高 (29.70 %)。
4.3.4 含不同濃度染料條件下東西台灣所篩選出之相同菌株(A. hydrophila)之 PHA 產量比較
由(表 4.9)可知對兩種菌株而言染料具部份生物毒性，因此對乾菌重產 生部份影響，亦可能由於染料對菌體產生之毒性影響，致使菌體在PHA 之 合成產生方面造成部分抑制，因此兩種菌株於含染料之環境中其PHA 產率 均較無染料環境之PHA 產率偏低；而比較東西台灣之菌株後可發現由東台 灣所篩選出之菌株(NIU01)具有較佳之 PHA 產生能力，並於含染料環境中 亦展現良好之抵抗染料毒性之能力，對其PHA 產率並無太大影響，而在先 前研究中(Chen et al., 2009b)亦發現 NIU01 具有較佳之抵抗染料生物毒性能 力，可能因此對染料濃度提高所造成之生物毒性反應較西台灣所篩選出之 菌株(YTl1)不明顯，因此 PHA 含量僅略微降低；而將兩種菌株之 PHA 產量 相比較亦可發現無論於何種染料濃度下 NIU01 具較高之產率，而在先前研 究中發現NIU01 具有良好之偶氮染料脫色能力，且其能力優於 YTl1，由此 推論PHA 之產量可能與菌體之脫色能力有相關聯，後續將依此發現更進一 步深入研究其他地區篩選出之菌株是否具相似情況，以及研究其PHA 產率 與染料濃度之相關聯因素為何則為後續研究之重點。
表 4.8 含高濃度(2000 mg L-1)芳香胺中間物(2AP、3AP)條件下對 PHA 產量之影響
對照組 2-Aminophenol (2AP) 3-Aminophenol (3AP) 限制條件
A. hydrophila NIU01(東台灣) A. hydrophila YTl1(西台灣) 染料濃度 0 4.76±0.12 1.40±0.46 29.25±9.03 4.06±0.18 0.89±0.40 21.67±8.78 300 2.49±2.14 0.53±0.30 25.65±10.12 3.00±0.68 0.42±0.16 14.84±8.52 500 3.54±0.44 0.83±0.06 23.87±4.77 3.31±0.08 0.46±0.15 13.87±4.76 700 3.76±0.08 0.88±0.25 23.24±6.10 2.817±0.001 0.37±0.14 13.23±4.85 1000 3.67±0.06 0.95±0.23 25.82±5.95 2.21±0.10 0.28±0.11 12.65±4.45
4.3.5 結論
由上述之各項討論可歸納出幾點結論，當菌體於氮磷比為 1:3 之比例 時具較高之乾菌重，而當氮磷比為1:9 時可得較高之 PHA 產率，於含有芳 香胺中間物之生物毒性環境下PHA 之產率略微受到抑制，而在含有染料之 環境中，隨染料濃度增加將造成部分生物毒性致使菌體之PHA 產率略微受 到影響，而脫色能力良好之菌株(NIU01)在含染料環境下仍能表現優出於其 他脫色菌株(YTl1)之良好 PHA 產率，因此推論 PHA 產率可能與染料脫色能 力相關聯。
5 總結與未來展望
總結上述各小節之結論，本研究中以染料結構將 7 種染料分為萘酚類 及非萘酚類兩大類，並以分子結構來分析結構對脫色難易程度之影響，總 體而言萘酚類較易脫色。並推出可快速計算固定化系統(ICS)之最大處理容 量之計算方法，以比較不同流速及基質濃度與染料種類之最大處理濃度，
流速在 45 ml hr-1 之處理容量最佳，其最大處理容量為 1514.6-1693.3 mg L-1，基質濃度為 0.3X LB 時其處理容量(976.4-1126 mg L-1)趨近於 0.5X LB(1127-1227 mg L-1) ， 而 不 同 染 料 之 處 理 容 量 其 順 序 亦 具 有 萘 酚 類 (RB5(2223.9); RR198(1923.9-2373.9); RG19(1173.9-1573.9); RB171 (873.9-1473.9))大於非萘酚類之趨勢(RY84(873.9-1243.9))，並希望未來可以 實場染整廢水進行實驗，以驗證實場應用之可行性，並探討菌體附著於載 體之菌相附著情況對處理效果之差異，以了解載體之孔隙度及比表面積對 菌體附著及處理效果之影響，並且延續資源回收再利用之理念，發展廢水 處理之同時產生 PHA 之綠色理念，探討於含染料或芳香胺中間物之環境 中，菌體產生PHA 之可行性，不同染料濃度對菌體將造成部份生物毒性，
略為抑制其PHA 的生產，而芳香胺中間物亦會對菌體造成生物毒性；基於 這些基本廢水處理回收之探索，期望能實際應用於染整廢水，並同步達到 PHA 之產出之可能性，而未來可就上述各項結論為研究起點，更進一步深 入研究其他地區篩選出之染料脫色菌株於含有染料之環境下是否具有與本
次研究相似情況，以及研究其PHA 產率與染料濃度之相關聯因素為何，添 加 其 他 種 芳 香 胺 中 間 物(4-Aminophenol, 3-Aminobenzoic acid, Aniline-2 sulfonic acid)是否能對菌體產生刺激 PHA 產率之效果，並將以發酵策略提 高PHA 產量，以各儀器(DSC, TGA, 拉力機等)測量其物性、化性之變化，
如此在染整廢水處理之同時產生資源回收再利用上提供更具永續發展之作 法。由此衍生出更具永續發展性之綠色概念。
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