固相微萃取使用方式

Appendix 5. Solid-phase microextraction steps.

(Daniel, 2003) 附錄 6、氣相層析裝置

Appendix 6. Gas chromatography device.

附錄 7、以 12 個性狀分析 30 大蒜品種間之相似性矩陣

Appendix 7. The similary matrix of 30 garlic cultivars based on 11 horticulture characters.

和美 古宅大

附錄 8、大蒜‘大片黑’葉片揮發性成分以固相微萃取法重複試驗所得之總離子層析圖。

Appendix 8. Total ion chromatogram of volatile components of garlic ‘Large Black Leaf’ replicated leaf samples by SPME-GC/MS.

附錄 9、大蒜‘廣西崇左市扶綏縣’葉片揮發性成分以固相微萃取法重複試驗所得之總離子層析圖。

Appendix 9. Total ion chromatogram of volatile components of garlic ‘Guang-Shi Tsung-Tzo-Shr Fu-Suei-Shien’ replicated leaf samples by SPME-GC/MS.

附錄 10、大蒜‘和美’鱗莖揮發性成分以固相微萃取法重複試驗所得之總離子層析圖。

Appendix 10. Total ion chromatogram of volatile components of garlic ‘He-Mei’ replicated cloves samples by SPME-GC/MS.

附錄 11、大蒜‘大片黑’鱗莖揮發性成分以固相微萃取法重複試驗所得之總離子層析圖。

Appendix 11. Total ion chromatogram of volatile components of garlic ‘Large Black Leaf’ replicated cloves samples by SPME-GC/MS.

附錄 12、大蒜‘宜蘭白’鱗莖揮發性成分以固相微萃取法重複試驗所得之總離子層析圖。

Appendix 12. Total ion chromatogram of volatile components of garlic ‘Yi-Lan White’ replicated cloves samples by SPME-GC/MS.

附錄 13、大蒜‘和美’鱗莖揮發性成分以溶劑萃取法重複試驗所得之總離子層析圖。

Appendix 13. Total ion chromatogram of volatile components of garlic ‘He-Mei’ replicated cloves samples by solvent extraction-GC/MS.

附錄 14、大蒜‘大片黑’鱗莖揮發性成分以溶劑萃取法重複試驗所得之總離子層析圖。

Appendix 14. Total ion chromatogram of volatile components of garlic ‘Large Black Leaf’ replicated cloves samples by solvent extraction-GC/MS.

附錄 15、大蒜‘宜蘭白’鱗莖揮發性成分以溶劑萃取法重複試驗所得之總離子層析圖。

Appendix 15. Total ion chromatogram of volatile components of garlic ‘Yi-Lan White’ replicated cloves samples by solvent extraction-GC/MS.

附錄 16、大蒜‘和美’鱗莖揮發性成分以溶劑萃取法重複試驗所得之總離子層析圖。

Appendix 16. Total ion chromatogram of volatile components of garlic ‘He-Mei’ duplicated cloves samples by solvent extraction-GC/MS.

附錄 17、大蒜‘大片黑’鱗莖揮發性成分以溶劑萃取法重複試驗所得之總離子層析圖。

Appendix 17. Total ion chromatogram of volatile components of garlic ‘Large Black Leaf’ duplicated cloves samples by solvent extraction-GC/MS.

附錄 18、大蒜‘宜蘭白’鱗莖揮發性成分以溶劑萃取法重複試驗所得之總離子層析圖。

Appendix 18. Total ion chromatogram of volatile components of garlic ‘Yi-Lan White’ duplicated cloves samples by solvent extraction-GC/MS.

附錄 19、大蒜‘和美’葉身揮發性成分於葉齡 20、40 及 60 天以固相微萃取法測定之總離子層析圖，化 合物編號對照表 17。

Appendix 19. Total ion chromatogram of volatile components of garlic ‘He-Mei’ leaf blade at leaf ages of 20, 40 and 60 days by SPME-GC/MS. The numbering of compounds relates to Table 17.

附錄 20、大蒜‘大片黑’葉身揮發性成分於葉齡 20、40 及 60 天以固相微萃取法測定之總離子層析圖，

化合物編號對照表 17。

Appendix 20. Total ion chromatogram of volatile components of garlic ‘Large Black Leaf’ leaf blade at leaf ages of 20, 40 and 60 days by SPME-GC/MS. The numbering of compounds relates to Table 17.

附錄 21、大蒜‘宜蘭白’葉身揮發性成分於葉齡 20、40 及 60 天以固相微萃取法測定之總離子層析圖，

化合物編號對照表 17。

Appendix 21. Total ion chromatogram of volatile components of garlic ‘Yi-Lan White’ leaf blade at leaf ages of 20, 40 and 60 days by SPME-GC/MS. The numbering of compounds relates to Table 17.

附錄 22、大蒜‘和美’葉身、葉鞘和鱗莖揮發性成分以固相微萃取法測定之總離子層析圖，化合物編號 對照表 17。

Appendix 22. Total ion chromatogram of volatile components of garlic ‘He-Mei’ leaf blade, leaf sheath and cloves by SPME-GC/MS. The numbering of compounds relates to Table 17.

附錄 23、大蒜‘大片黑’葉身、葉鞘和鱗莖揮發性成分以固相微萃取法測定之總離子層析圖，化合物編 號對照表 17。

Appendix 23. Total ion chromatogram of volatile components of garlic ‘Large Black Leaf’ leaf blade, leaf sheath and cloves by SPME-GC/MS. The numbering of compounds relates to Table 17.

附錄 24、大蒜‘宜蘭白’葉身、葉鞘和鱗莖揮發性成分以固相微萃取法測定之總離子層析圖，化合物編 號對照表 17。

Appendix 24. Total ion chromatogram of volatile components of garlic ‘Yi-Lan-Bai’ leaf blade, leaf sheath and cloves by SPME-GC/MS. The numbering of compounds relates to Table 17.

附錄 25、大蒜‘和美’蒜皮和蒜瓣揮發性成分以固相微萃取法測定之總離子層析圖，化合物編號對照表 17。

Appendix 25. Total ion chromatogram of volatile components of garlic ‘He-Mei’ clove peels and cloves by SPME-GC/MS. The numbering of compounds relates to Table 17.

附錄 26、大蒜‘大片黑’蒜皮和蒜瓣揮發性成分以固相微萃取法測定之總離子層析圖，化合物編號對照 表 17。

Appendix 26. Total ion chromatogram of volatile components of garlic ‘Large Black Leaf’ clove peels and cloves by SPME-GC/MS. The numbering of compounds relates to Table 17.

附錄 27、兩個不同來源的大蒜‘和美’鱗莖揮發性成分以固相微萃取法所測得之總離子層析圖。（上）

草屯市場、（下）台中區農業改良場。化合物編號對照表 17。

Appendix 27. Total ion chromatogram of volatile components of garlic ‘He-Mei’ bulbs from two sources.

(above) open market at Tsao-Tun, (below) Taichung District Agriculture Research and Extension Station. The numbering of compounds relates to Table 17.

附錄 28、兩個不同來源的大蒜‘大片黑’鱗莖揮發性成分以固相微萃取法所測得之總離子層析圖。（上）

草屯市場、（下）台中區農業改良場。化合物編號對照表 17。

Appendix 28. Total ion chromatogram of volatile components of garlic ‘Large Black Leaf’ bulbs from two sources. (above) open market at Tsao-Tun, (below) Taichung District Agriculture Research and Extension Station. The numbering of compounds relates to Table 17.

附錄 29、大蒜‘和美’和‘古宅大蒜’葉身揮發性成分以固相微萃取法測定之總離子層析圖，化合物編號對 照表 17。

Appendix 29. Total ion chromatogram of volatile components of garlic ‘He-Mei’ and ‘Gu-Zai’ leaf blade by SPME-GC/MS. The numbering of compounds relates to Table 17.

附錄 30、大蒜‘混香蒜’和‘大片黑’葉身揮發性成分以固相微萃取法測定之總離子層析圖，化合物編號對 照表 17。

Appendix 30. Total ion chromatogram of volatile components of garlic ‘Hun Hsiang Suan’ and ‘Large Black Leaf’ leaf blade by SPME-GC/MS. The numbering of compounds relates to Table 17.

附錄 31、大蒜‘廣西仁東玉林’和‘四川南蒜’葉身揮發性成分以固相微萃取法測定之總離子層析圖，化合 物編號對照表 17。

Appendix 31. Total ion chromatogram of volatile components of garlic ‘Guang-Shi Jen-Dong Yu-Lin’ and

‘Sz-Chuan Nan Suan’ leaf blade by SPME-GC/MS. The numbering of compounds relates to Table 17.

附錄 32、大蒜‘四川新都紫皮蒜’和‘正月早新繁市場’葉身揮發性成分以固相微萃取法測定之總離子層析 圖，化合物編號對照表 17。

Appendix 32. Total ion chromatogram of volatile components of garlic ‘Sz-chuan Hsin-Du Purple Peel’ and

‘Early January Hsin-Fan Market’ leaf blade by SPME-GC/MS. The numbering of compounds relates to Table 17.

附錄 33、大蒜‘越南紅膜早熟’和‘廣西崇左市扶綏縣’葉身揮發性成分以固相微萃取法測定之總離子層析 圖，化合物編號對照表 17。

Appendix 33. Total ion chromatogram of volatile components of garlic ‘Viet-Nam Red Peel Early’ and

‘Guang-Shi Tsung-Tzo-Shr Fu-Suei-Shien’ leaf blade by SPME-GC/MS. The numbering of compounds relates to Table 17.

附錄 34、大蒜‘越南’和‘雲南昆明瓣蒜’葉身揮發性成分以固相微萃取法測定之總離子層析圖，化合物編 號對照表 17。

Appendix 34. Total ion chromatogram of volatile components of garlic ‘Viet-Nam’ and ‘Yun-Nan Kun-Ming Ban Suan’ leaf blade by SPME-GC/MS. The numbering of compounds relates to Table 17.

附錄 35、大蒜‘大里彌度獨蒜’和‘廣州江南洱沅獨蒜’葉身揮發性成分以固相微萃取法測定之總離子層析 圖，化合物編號對照表 17。

Appendix 35. Total ion chromatogram of volatile components of garlic ‘Da-Li Mi-Du Single clove’ and

‘Guang-Jou Jiang-Nan Er-Yuan Single clove’ leaf blade by SPME-GC/MS. The numbering of compounds relates to Table 17.

附錄 36、大蒜‘嘉定 2 號’和‘雲南昆明王旗營紫蒜’葉身揮發性成分以固相微萃取法測定之總離子層析 圖，化合物編號對照表 17。

Appendix 36. Total ion chromatogram of volatile components of garlic ‘Jia-Ding No.2’ and ‘Yun-Nan Kun-Ming Wang-Chi-Yin Purple’ leaf blade by SPME-GC/MS. The numbering of compounds relates to Table 17.

附錄 37、大蒜‘廣東梯雲獨蒜’和‘河南白蒜’葉身揮發性成分以固相微萃取法測定之總離子層析圖，化合 物編號對照表 17。

Appendix 37. Total ion chromatogram of volatile components of garlic ‘Guang-Dong Ti-Yun Single Clove’

and ‘He-Nan White’ leaf blade by SPME-GC/MS. The numbering of compounds relates to Table 17.

附錄 38、大蒜‘北京新發地’和‘彭州正月早’葉身揮發性成分以固相微萃取法測定之總離子層析圖，化合 物編號對照表 17。

Appendix 38. Total ion chromatogram of volatile components of garlic ‘Bei-Jing Hsin-Fa-Di’ and ‘Peng-Jou January Early’ leaf blade by SPME-GC/MS. The numbering of compounds relates to Table 17.

附錄 39、大蒜‘彭州丹景山二月早’和‘雲南昆明王旗營三瓣蒜’葉身揮發性成分以固相微萃取法測定之總 離子層析圖，化合物編號對照表 17。

Appendix 39. Total ion chromatogram of volatile components of garlic ‘Peng-Jou Dan-Jin-Shan February Early’ and ‘Yun-Nan Kun-Ming Wang-Chi-Yin Three Cloves’ leaf blade by SPME-GC/MS. The numbering of compounds relates to Table 17.

附錄 40、大蒜‘彭州溫二早’和‘韓國暖地型’葉身揮發性成分以固相微萃取法測定之總離子層析圖，化合 物編號對照表 17。

Appendix 40. Total ion chromatogram of volatile components of garlic ‘Peng-Jou Wen-Er-Zao’ and ‘Korea Warm Site’ leaf blade by SPME-GC/MS. The numbering of compounds relates to Table 17.

附錄 41、大蒜‘韓國昌寧’和‘蒼山蒲蒜’葉身揮發性成分以固相微萃取法測定之總離子層析圖，化合物編 號對照表 17。

Appendix 41. Total ion chromatogram of volatile components of garlic ‘Korea Chang-Nin’ and ‘Tsan-Shan Pu-Suan’ leaf blade by SPME-GC/MS. The numbering of compounds relates to Table 17.

附錄 42、大蒜‘芳苑花蒜’和‘宜蘭白’葉身揮發性成分以固相微萃取法測定之總離子層析圖，化合物編號 對照表 17。

Appendix 42. Total ion chromatogram of volatile components of garlic ‘Fan-Yuan Scape’ and ‘Yi-Lan White’

leaf blade by SPME-GC/MS. The numbering of compounds relates to Table 17.

附錄 43、大蒜‘四色菊府’和‘正月早彭州’葉身揮發性成分以固相微萃取法測定之總離子層析圖，化合物 編號對照表 17。

Appendix 43. Total ion chromatogram of volatile components of garlic ‘Sz-Se Ju-Fu’ and ‘Early January Peng-Jou’ leaf blade by SPME-GC/MS. The numbering of compounds relates to Table 17.

附錄 44、大蒜 30 個品種以 SPME-GC/MS 測定葉片揮發性含硫化合物之校正量^z

Appendix 44. Calibrated abundance of volatile sulfur-containing compounds obtained from leaves of 30 garlic cultivars by SPME-GC/MS.

化合物

1 2,536,298 2,619,159 1,929,362 2,809,593 2,308,548 2,194,753 2,024,106 3,072,294 1,210,007 2,526,340 51,731 540,577 1,558,688 1,982,282 1,892,400 229,244 1,908,621 - 723,775 1,771,246 743,535 606,706 2,920,590 1,542,983 2,639,621 699,940 391,771 499,043 2,328,411 787,976 3 -^x 314,106 - - - 253,406 - - - - - 254,394 1,732,104 239,887 361,406 - - 2,850,575 3,612,445 - 694,884 - 402,714 850,343 - 273,935 1,032,607 370,187 4 - - - 2,063,386 - - - 2,850,305 - 2,304,021 2,484,995 348,378 782,469 - 718,449 1,962,257 2,709,914 1,226,632 - 312,168 1,207,124 1,766,856 420,311 503,625 - 247,188 5 57,080,992 56,413,026 57,848,392 53,724,814 56,598,379 51,461,281 43,709,145 51,958,745 20,071,155 63,167,674 15,111,761 23,651,556 32,086,001 33,033,867 38,175,622 15,725,955 26,764,212 11,487,030 23,091,296 29,349,074 37,503,248 36,904,583 41,272,911 21,338,769 32,094,646 31,527,844 13,616,033 18,947,384 35,416,868 23,838,078 6 3,283,414 2,481,728 3,593,965 2,692,703 3,418,426 2,306,590 2,297,067 2,401,964 - 3,930,863 659,818 1,860,235 1,524,057 1,008,878 2,637,821 1,476,685 1,669,471 267,019 1,976,728 1,993,826 2,491,610 2,989,947 1,718,001 791,386 1,466,205 1,758,772 1,355,399 1,921,100 365,472 1,374,705 7 11,335,054 8,735,477 10,336,879 4,715,856 5,057,039 3,480,286 6,474,091 8,456,938 345,236 7,656,054 1,664,795 4,336,328 2,261,546 3,495,655 6,542,675 4,082,757 7,432,226 1,267,843 9,246,844 6,920,790 9,506,880 13,526,221 3,957,013 2,440,959 4,099,783 6,585,238 4,708,435 6,766,735 1,542,820 4,928,096 10 - - - 566,164 - - - 1,180,570 - 781,476 2,624,616 293,944 553,451 189,976 - 6,486,213 13,361,793 322,340 - - 737,849 1,749,480 316,458 193,786 - 312,787

11 - - - - - - - 194,998 - - - 261,526 - 244,144 255,211 - - - - 278,152 434,550 145,565 - - 149,278 161,931 - - -

-12 - - - - - - - - - - - - - - - - - - - - - 107,578 - - - - - - -

-13 - - - 1,402,755 - - - 1,845,531 - 1,499,610 1,540,963 - 884,597 - 724,974 1,287,312 2,499,218 1,096,297 - - 1,257,355 1,346,453 - - - -14 - 142,923 - 300,195 - - 725,558 2,627,912 484,110 - 166,763 4,124,738 350,372 2,998,622 3,454,360 755,140 1,305,664 232,283 1,051,274 3,299,490 6,865,806 1,956,186 358,026 603,545 1,994,211 2,436,265 777,346 876,030 - 493,746

16 - - - - - - - 619,038 - - - 465,890 - 381,567 528,822 - - - - 432,137 953,367 488,137 - - 573,974 272,757 - - -

-17 - 178,059 115,428 135,815 144,381 96,736 333,728 925,489 - 137,345 - 1,022,201 91,348 970,380 1,206,820 159,712 351,946 - 254,380 1,227,750 2,538,609 534,133 128,925 128,223 608,996 672,743 148,694 227,757 - 115,123 19 166,189 213,663 151,557 80,391 111,635 114,747 - 594,421 - 194,992 - 549,943 103,919 277,617 630,356 345,318 358,196 - 469,165 555,488 719,753 733,563 362,892 154,529 294,597 427,730 216,800 376,004 - 147,684 21 - 229,939 - 307,536 - - 427,556 2,604,089 215,530 174,531 - 1,557,832 312,763 1,393,895 2,089,288 544,964 822,417 - 663,350 1,760,354 2,845,533 1,112,731 285,157 703,372 1,095,217 1,584,989 557,823 765,211 - 574,423 22 247,970 204,902 200,113 361,205 203,503 200,268 228,603 387,603 221,445 181,258 344,462 290,241 294,146 292,207 303,095 290,291 250,738 345,311 262,167 235,362 - 197,232 197,713 375,918 263,431 304,517 342,355 354,491 268,941 417,776

25 - - - - - - - 384,263 - - - - - - 137,233 - - - - - - - - - - - - - -

-z: 樣品取自台中區農業改良場田區，取樣葉片葉齡為 40 天

All leaf samples were collected from Taichung District Agricultural Research and Extension Station. Sample leaf is of the age 40 days.

y: peak number and its corresponding compound are same as listeded in Table 17

x: ‘-’ not detected

附錄 45、以固相微萃取法所得之 17 個葉片揮發性含硫化合物分析 30 個大蒜品種間之相似性矩陣

Appendix 45. The similarly matrix of 30 garlic cultivars based on 17 volatile sulfur-containing components obtained from SPME.

和美 古宅大 越南 0.8162 0.7143 0.7425 0.7908 0.7411 0.7421 0.8309 0.4165 0.7704 0.6666 1

雲南昆明瓣 蒜

0.3155 0.453 0.3318 0.4147 0.3309 0.3304 0.4101 0.9823 0.3506 0.3644 0.3574 1 大里彌度獨

蒜

0.3756 0.3154 0.4133 0.534 0.4127 0.4122 0.5013 0.6739 0.4078 0.4615 0.4126 0.6481 1 廣州江南洱

沅獨蒜 0.7642 0.9059 0.821 0.9995 0.8214 0.8217 0.9212 0.4811 0.7542 0.9035 0.7811 0.4132 0.535 1 嘉定２號 0.3508 0.4981 0.381 0.4857 0.3813 0.3812 0.4743 0.7648 0.4113 0.4216 0.4 0.8093 0.8397 0.4851 1 雲南昆明王

旗營紫蒜

0.5553 0.7978 0.5932 0.7306 0.5919 0.5918 0.6686 0.5409 0.5454 0.6553 0.5685 0.5792 0.47 0.7317 0.6328 1 廣東梯雲獨 韓國暖地型 0.6768 0.793 0.7278 0.9035 0.728 0.7284 0.8329 0.5258 0.695 0.8109 0.6972 0.4593 0.6103 0.9046 0.5496 0.8056 0.7087 0.4622 0.8906 0.4866 0.2939 0.5856 0.7826 1

韓國昌寧 0.3812 0.5197 0.4025 0.495 0.4025 0.4025 0.4793 0.7659 0.4134 0.4394 0.4183 0.8115 0.8229 0.4936 0.9976 0.6377 0.7421 0.3874 0.6048 0.9901 0.8272 0.5826 0.5173 0.543 1 蒼山蒲蒜 0.3544 0.5133 0.3761 0.4734 0.3748 0.3744 0.4546 0.7584 0.3845 0.4173 0.3897 0.8153 0.807 0.4729 0.9938 0.6636 0.7629 0.4116 0.593 0.9959 0.835 0.5611 0.5116 0.5289 0.9944 1

芳苑花蒜 0.6084 0.6802 0.6531 0.8086 0.6522 0.652 0.7443 0.5404 0.609 0.7234 0.6284 0.5194 0.6831 0.8096 0.5978 0.8951 0.7819 0.6248 0.7707 0.5754 0.3913 0.6228 0.6681 0.8976 0.5906 0.5985 1 宜蘭白 0.565 0.808 0.605 0.7422 0.6038 0.6036 0.6819 0.5441 0.5564 0.6683 0.5777 0.5785 0.4713 0.7428 0.634 0.9993 0.8663 0.5607 0.6683 0.6576 0.4712 0.3536 0.8077 0.8192 0.6391 0.6625 0.8967 1 四色菊府 0.794 0.7247 0.7201 0.576 0.7194 0.721 0.6081 0.3419 0.5566 0.6449 0.7797 0.3375 0.0844 0.5594 0.3422 0.5427 0.4888 0.5319 0.3716 0.3542 0.1193 0.0684 0.7096 0.4641 0.3808 0.3626 0.379 0.5529 1

正月早彭州 0.575 0.8091 0.6066 0.7365 0.6058 0.6059 0.6862 0.5437 0.5737 0.6681 0.5933 0.5806 0.4584 0.7362 0.6338 0.9976 0.8664 0.5761 0.6474 0.6602 0.4652 0.339 0.8085 0.8018 0.6408 0.6628 0.8877 0.9979 0.5854 1

附錄 46、大蒜‘和美’和‘古宅大蒜’鱗莖揮發性成分以固相微萃取法測定之總離子層析圖，化合物編號對 照表 17。

Appendix 46. Total ion chromatogram of volatile components of garlic ‘He-Mei’ and ‘Gu-Zai’ cloves by SPME-GC/MS. The numbering of compounds relates to Table 17.

附錄 47、大蒜‘混香蒜’和‘大片黑’鱗莖揮發性成分以固相微萃取法測定之總離子層析圖，化合物編號對 照表 17。

Appendix 47. Total ion chromatogram of volatile components of garlic ‘Hun Hsiang Suan’ and ‘Large Black Leaf’ cloves by SPME-GC/MS. The numbering of compounds relates to Table 17.

附錄 48、大蒜‘廣西仁東玉林’和‘四川南蒜’鱗莖揮發性成分以固相微萃取法測定之總離子層析圖，化合 物編號對照表 17。

Appendix 48. Total ion chromatogram of volatile components of garlic ‘Guang-Shi Jen-Dong Yu-Lin’ and

‘Sz-Chuan Nan Suan’ cloves by SPME-GC/MS. The numbering of compounds relates to Table 17.