為了進一步地將白血球分類，以實現全血細胞計數(CBC)的功能，本實驗以 4.5μm 微珠以及 10μm 微珠進行模擬捕捉白血球及較大顆的罕見細胞分佈測試，圖 4-13(A)為只流入 4.5μm 微珠溶液之捕捉影像；圖 4-13(B) 為只流入 10μm 微珠溶 液之捕捉影像，這兩張影像說明了圓形捕捉區域能夠個別地兩種微珠；而圖 4-13(C) 則是流入4.5μm 及 10μm 微珠混合溶液之捕捉影像，從影像上發現 10μm 微珠只有 被第四圈以內微柱結構捕捉，而4.5μm 微珠則是被最外面的四圈微柱結構所捕捉，
這說明了圓形捕捉區域也能將兩種不同尺寸的混合微珠進行分類捕捉，使本微流 道晶片具有捕捉血液中稀少細胞的可能性。
圖 4-13 圓形捕捉區域捕捉(A)4.5μm 微珠(B)10μm 微珠(C)4.5μm 及 10μm 微珠。
第 5 章 結論與未來展望
在白血球捕捉的部分，以 Calcein-AM 螢光染劑使被微柱捕捉之白血球能夠進 行影像上的計數，之字形捕捉區域能夠從約 10μL 之全血中捕捉到約 3000 顆的白
結構會產生導角的效果，夾角的地方會變得較圓鈍，導致分離流道的分離效 果不如預期。因此在未來新的微流道設計畫圖檔時需將導角的問題考慮進 去，使導角的效果抵消以解決此問題。
2. 在實驗的過程中，一直為雜質流入流道使得流道卡死的問題所困擾，雜質來 源來自於 Pluronic 溶液以及病人的血液，因此須在流道的入口處增加過濾 雜質的微柱結構，避免雜質導致實驗失敗。另一部分，在流道入口針頭的部 分加入磁珠攪拌器或振盪器，讓實驗中的血液能保持全血的狀態。
3. 本論文所設計之之字形捕捉微區域之捕捉效率量測並未最佳化，在未來將 縮短捕捉區域的左右寬度，或以不同的排列方式排列微柱。此外增加樣本實 驗的數據後，預期能夠輕易地從捕捉到白血球的微柱數量，甚至只需觀察捕 捉區域，就可以得知捕捉到的白血球數量。
4. 目前的全血處理微流道晶片仍是在實驗室的環境下進行，且其操作需經過 專業訓練，未來希望能夠將其應用在定點照護。故能加快具體實現的方式有：
未來以新的微流道設計進行目前已有的實驗來驗證其表現、簡化整個處理 流程使每個人都能輕易使用、提高微流道表現得一致性以降低人為的誤差。
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