表 一、完熟長實金柑不同溶劑萃取物之 DPPH 自由基清除能力
Table 1. Scavenging effects on DPPH radical of various solvent extracts from pulp and peel of Fortunella margarita Swingle with 100% maturety.
DPPH radical scavenging activity(%)
Sample
(mg /mL)
Methanol Extract Water Extract Ethyl Acetate Extract
* Means(n=3) with different superscripts (a-e,x-z) in separate sample are significantly different (p<0.05).
表 二、長實金柑不同萃取方法之萃取物 DPPH 自由基清除能力
Table 2. Scavenging effects on DPPH radical of methanolic extracts from pulp and peel of Fortunella margarita Swingle with various methods.
DPPH radical scavenging activity(%)
Sample(mg /mL)
Extraction 24 hrs Extraction 1 hr for three times Pulp
a-f Means(n=3) with different superscripts in a raw of separate sample are significantly different (p<0.05).
表 三、 完熟長實金柑果皮及果肉萃取物清除 DPPH 自由基能力之 IC50
值
Table 3. The IC50 of extracts from Fortunella margarita Swingle with 100% maturety peel and pulp as related to DPPH free radical scavenging capacity.
IC50 of DPPH scavenging effect (mg/mL)
Kumquat
Methanol Extract Water Extract Ethyl Acetate Extract Peel 5.01 5.31 6.64 Pulp 4.93 5.28 6.79 BHA 0.013
表 四、不同成熟度金柑一般成分分析
Table 4. Proximate composition analysis of Fortunella margarita Swingle (FMS) and Fortunella japonica Swingle (FJS)
with different maturities.
Maturity (%) Moisture(%) Crude protein(%) Crude fat(%) Ash(%) Carbohydrate(%)
FMS
50 85.51± 2.36b 0.90±0.05abc 0.28±0.01a 0.30±0.02a 13.01±1.23a 60 87.20±3.63ab 0.97±0.12abc 0.22±0.01ab 0.32±0.03a 11.29±0.75ab 70 86.20±2.23ab 1.05±0.20ab 0.20±0.00ab 0.35±0.02a 12.20±0.89ab 80 86.81±2.05ab 1.04±0.06ab 0.21±0.00ab 0.32±0.03a 11.63±0.56ab 90 85.80±2.63b 0.82±0.07cd 0.15±0.00bc 0.33±0.06a 12.90±0.96a 100 85.90±1.20b 1.07±0.05a 0.28±0.02a 0.32±0.09a 12.43±0.23ab FJS
30 85.70±2.08b 0.90±0.05abc 0.10±0.00b 0.50±0.06a 12.80±1.02a 50 87.08±3.06ab 0.86±0.02bc 0.24±0.02a 0.55±0.05a 11.27±0.96ab 70 86.49±2.54ab 0.90±0.06abc 0.10±0.01b 0.51±0.07a 12.00±0.56ab 100 87.89±4.38a 0.79±0.09c 0.20±0.02a 0.50±0.06a 10.71±0.58b
a-d Means(n=3) with different superscripts in a column of separate sample are significantly different (p<0.05).
表 五、不同成熟度金柑果皮與果肉甲醇萃取物之總多酚含量
Table 5. Total polyphenol contents of methanolic extracts from peel and pulp of kumquat with different maturities.
Total polyphenol contents of kumquat (mg/100g dry weight)
Maturity
(%) Peel Pulp
FMSe
50 1277.03±20.63a 1145.03±34.3a 60 884.47±32.60b 816.76±25.7b 70 867.33±42.36b 748.20±25.7bc 80 850.19±68.61b 720.77±25.7c 90 797.91±25.71b 674.48±8.61c 100 806.48±62.82b 653.91±42.9c FJSf
30 2132.42±34.32a 947.04±43.71a 50 1809.29±42.06b 853.62±68.57a 70 1670.44±94.37c 861.33±8.66a 100 1453.60±20.00d 850.19±12.00a
a-d Means(n=3) with different superscripts in a column of separate sample are significantly different (p<0.05).
e FMS: Fortunella margarita Swingle
f FJS :Fortunella japonica Swingle
表 六、不同成熟度金柑果皮與果肉甲醇萃取物之總類黃酮含量
Table 6. Total flavonoids contents of methanolic extracts from peel and pulp of kumquat with different maturities.
Total flavonoids contents of kumquat(mg/100g dry weight)
Maturity
(%) peel pulp
FMSd
50 430.63±1.50a 82.53±17.22a 60 358.76±14.97ab 97.13±3.37a 70 374.86±1.10ab 92.26±2.99a 80 351.65±10.85b 82.54±22.46a 90 238.24±24.45c 84.78±15.35a 100 307.11±20.96bc 100.50±2.25a FJSe
30 913.47±14.60bc 98.26±0.56bc 50 852.84±10.11c 87.96±5.61c 70 953.90±14.60b 116.66±2.25b 100 1351.40±19.09a 137.55±3.93a
a-c Means(n=3) with different superscripts in a column of separate sample are significantly different (p<0.05).
d FMS: Fortunella margarita Swingle
e FJS :Fortunella japonica Swingle
表 七、不同成熟度金柑果皮與果肉甲醇萃取物之總花青素含量
Table 7. Total anthocyanin contents of methanolic extracts from peel and pulp of kumquat with different maturities.
Total anthocyanidins content (mg/100g dry weight)
Maturity
(%) Peel Pulp
FMSe
50 0.86±0.14a 0.01±0.00b 60 0.09±0.02c 0.11±0.06a 70 0.29±0.11b N.D.
80 0.11±0.04d N.D.
90 N.D.g N.D.
100 N.D. N.D.
FJSf
30 12.71±0.73b 1.22±0.23a 50 15.97±1.71a N.D.
70 6.98±1.00c N.D.
100 6.98±1.18c N.D.
a-d Means(n=3) with different superscripts in a column of separate sample are significantly different (p<0.05).
e FMS: Fortunella margarita Swingle
f FJS :Fortunella japonica Swingle
g N.D.:Not detected
表 八、不同成熟度金柑果皮與果肉甲醇萃取物之總類胡蘿蔔素含量 Table 8. The caroteneoids contents of methanolic extracts from peel and pulp of kumquat with different maturities.
Caroteneoids contents of kumquat (mg/100g dry weight)
Matuity
(%) peel pulp
FMSg
50 16.38±0.00e 2.45±0.00c 60 13.46±0.00f 2.64±0.01c 70 26.54±0.04b 2.89±0.02bc 80 20.79±0.00d 3.30±0.02bc 90 23.25±0.01c 3.10±0.02b 100 36.60±0.04a 4.86±0.02a FJSh
30 72.03±0.02a 9.73±0.01a 50 37.69±0.02c 7.75±0.02b 70 31.88±0.02c 9.35±0.04a 100 65.36±0.08b 11.19±0.06a
a-f Means(n=3) with different superscripts in a column of separate sample are significantly different (p<0.05).
g FMS: Fortunella margarita Swingle
h FJS :Fortunella japonica Swingle
表 九、不同成熟度金柑果皮與果肉甲醇萃取物之果糖、葡萄糖和蔗糖含量
Table 9. The Fructose, Glucose and Sucrose contents of methanolic extracts from peel and pulp of Kumquat with different Maturities.
Contents of kumquat ( mg/mg dry weight)
Fructose Sucrose Glucose Total Matuity
(%) Peel Pulp Peel Pulp Peel Pulp Peel Pulp
FMSe
50 0.18±0.03a 0.10±0.02a 0.17±0.01c 0.12±0.00d 0.16±0.03ab 0.08±0.01a 0.35±0.02b 0.18±0.01b 60 0.17±0.03a 0.11±0.02a 0.13±0.03d 0.15±0.02cd 0.14±0.03bc 0.09±0.02a 0.31±0.03c 0.20±0.02b 70 0.17±0.02a 0.13±0.02a 0.19±0.00b 0.16±0.02bc 0.15±0.02abc 0.10±0.02a 0.32±0.01c 0.23±0.02a 80 0.20±0.02a 0.13±0.02a 0.26±0.01a 0.21±0.01a 0.18±0.02a 0.10±0.01a 0.38±0.01a 0.23±0.01a 90 0.15±0.03a 0.14±0.03a 0.15±0.05cd 0.19±0.06ab 0.12±0.03c 0.10±0.03a 0.27±0.04d 0.24±0.04a 100 0.15±0.04a 0.13±0.02a 0.25±0.01a 0.18±0.01abc 0.14±0.02b 0.09±0.02a 0.29±0.02c 0.22±0.01ab FJSf
30 0.04±0.01b 0.05±0.02b 0.00±0.01b 0.02±0.01c 0.06±0.02b 0.05±0.02b 0.10±0.01d 0.09±0.01c 50 0.07±0.04b 0.02±0.01b 0.01±0.01b 0.02±0.00c 0.08±0.04b 0.02±0.02b 0.15±0.02c 0.05±0.01d 70 0.14±0.02a 0.05±0.02b 0.03±0.01a 0.06±0.00b 0.14±0.03a 0.05±0.02b 0.28±0.01b 0.11±0.01b 100 0.16±0.03a 0.11±0.03a 0.04±0.00a 0.09±0.01a 0.16±0.03a 0.10±0.03a 0.32±0.02a 0.21±0.02a
a-d Means(n=3) with different superscripts in a column of separate sample are significantly different (p<0.05).
e FMS: Fortunella margarita Swingle f FJS :Fortunella japonica Swingle
表 十、不同成熟度金柑果皮甲醇萃取物之草酸與抗壞血酸含量
Table 10.The oxalic acid and ascorbic acid contents of methanolic extracts from peel of Kumquat with different maturities .
contents of kumquat (mg/100g dry weight)
Maturity(%)
Oxalic acid Ascorbic acid FMSg
50 390.32±5.72b 361.60±2.08a 60 299.62±2.48e 355.44±1.50b 70 342.84±1.54d 330.92±1.86c 80 511.46±5.76a 321.64±1.81d 90 369.12±15.32c 307.76±1.78e 100 294.74±6.10e 263.36±2.11f FJSh
30 383.97±10.92b 1013.88±2.16a 50 434.16±4.62a 531.92±0.91b 70 267.14±10.82d 348.88±1.48c 100% 345.31±2.83c 221.08±2.26d
a-f Means(n=3) with different superscripts in a column of separate sample are significantly different (p<0.05).
g FMS: Fortunella margarita Swingle
h FJS :Fortunella japonica Swingle
表 十一、不同成熟度金柑果肉甲醇萃取物之草酸、抗壞血酸與檸檬酸 含量
Table 11.The oxalic acid , ascorbic acid and citric acid contents of methanolic extracts from pulp of Kumquat with different maturities.
contents of kumquat (mg/100g dry weight)
Maturity
(%) Oxalic acid Ascorbic acid Citric acid FMSf
50 385.15±1.68a 407.93±17.12a 2050.93±14.72a 60 395.88±3.65a 178.08±3.26b 1517.66±11.26b 70 305.89±2.43d 164.99±1.18c 1348.62±11.75c 80 364.21±7.83b 160.71±6.24c 1073.91±28.85d 90 334.74±1.14c 110.19±0.27d 1034.01±22.57e 100 388.93±2.55a 100.35±12.31d 1050.93±27.74de FJSg
30 390.28±19.08b 309.44±17.29a 3409.17±17.29a 50 259.12±3.17d 200.22±6.47b 3212.72±6.67b 70 338.30±6.18c 202.82±20.87b 3014.55±20.87c 100 438.87±5.43a 86.23±14.86c 2096.99±14.86d
a-e Means(n=3) with different superscripts in a column of separate sample are significantly different (p<0.05).
f FMS: Fortunella margarita Swingle
g FJS :Fortunella japonica Swingle
表 十二、不同成熟度長實金柑果皮與果肉甲醇萃取物之成分變化與抗氧化活性之相關性
Table 12.Pearson correlation coefficients between antioxidative activities and related components in peel and pulp pf FMS with different maturities
Scavenging effects on DPPH radical Reducing power Chealting effects on Fe+2 Peel of FMS1
Total polyphenol contents 0.6899* 0.8474*** 0.3612 Total flavonoids contents 0.8725*** 0.8641*** 0.6621* Total anthocyanidins contents 0.7212** 0.8396*** 0.3955
Total caroteneoids contents 0.7628** 0.6368* 0.7601**
Organic acids2 0.5126 0.2138 0.3341
Saccharides3 0.6145* 0.3990 0.3915
Pulp of FMS
Total polyphenol contents 0.7357** 0.7554** 0.3578 Total flavonoids contents 0.7390** 0.4376 0.3228
Total anthocyanidins contents 0.2076 0.3050 0.4707 Total caroteneoids contents 0.8740*** 0.9050*** 0.0316
Organic acids 0.6736* 0.7654** 0.1910
Saccharides 0.5170 0.3692 0.4595
1FMS : Fortunella margarita Swingle ;2Organic acids: Oxalic acid+Ascorbic acid+Citric acid ;3Saccharides:
Fructose+Sucrose+Glucose
* : ( p< 0.05); **: ( p< 0.01); ***: (p< 0.001)
表 十三、不同成熟度圓金柑果皮與果肉甲醇萃取物之成分變化與抗氧化活性之相關性
Table 13. Pearson correlation coefficients between antioxidative activities and related components in peel and pulp pf FJS with different maturities
Content Scavenging effects on DPPH radical Reducing power Chealting effects on Fe+2 Peel of FJS1
Total polyphenol contents 0.6244 0.1396 0.8243* Total flavonoids contents 0.2245 0.3176 0.9845***
Total anthocyanidins contents 0.004 0.4292 0.6333 Total caroteneoids contents 0.8323* 0.7301* 0.3282
Organic acids2 0.7817* 0.8186* 0.9693***
Saccharides3 0.4438 0.3332 0.7837*
Pulp of FJS
Total polyphenol contents 0.6840 0.4404 0.1746 Total flavonoids contents 0.1546 0.4321 0.1068 Total anthocyanidins contents 0.7455* 0.3550 0.0755
Total caroteneoids contents 0.5651 0.2456 0.3294
Organic acids 0.0141 0.7705* 0.472
Saccharides 0.3758 0.5800 0.3162
1 FJS :Fortunella japonica Swingle ; 2Organic acids: Oxalic acid+Ascorbic acid+Citric acid ; 3Saccharides:
Fructose+Sucrose+Glucose
* : ( p< 0.05); **: ( p< 0.01); ***: (p< 0.001)
表 十四、不同成熟度長實金柑果皮與果肉甲醇萃取物之類黃酮組成
Table 14. Flavonoids composition in peel and pulp of Fortunella margarita Swingle(FMS) with different maturities Contents of kumquat ( mg/100g dry weight )
Maturity
(%) Rutin Quercertin Kaempferol Hesperidin Hesperetin Naringin Naringenin Neohesperidin Diosmin peel
50 N.D.f 42.40±0.41a 1.29±0.01bc N.D. 0.24±0.00c 0.59±0.04a N.D. 1.80±0.06a 70.93±0.48a 60 N.D. 32.97±0.09d 1.18±0.02c N.D. 0.16±0.00e N.D. 0.52±0.00a 1.19±0.11b 47.31±0.38d 70 N.D. 39.14±0.30b 1.35±0.02bc N.D. 0.22±0.00d N.D. 0.49±0.00b 1.21±0.06b 63.81±1.01b 80 0.86±0.06b 34.51±0.20cd 1.93±0.04a 1.55±0.09b 0.22±0.02d 0.15±0.02c 0.49±0.00b 0.21±0.04d 56.25±0.60c 90 0.91±0.02a 35.38±0.18c 1.61±0.02ab 1.13±0.03c 0.27±0.03b 0.22±0.03b 0.37±0.10c 0.73±0.07c 55.57±0.70cd 100 0.67±0.16c 35.31±0.10c 1.83±0.01a 3.84±0.06a 0.29±0.01a 0.23±0.01b 0.14±0.01d 0.26±0.06d 54.24±0.29c pulp
50 N.D. 33.62±0.23d 1.24±0.03c 0.10±0.04d 0.11±0.02e 0.41±0.04cd 1.87±0.02a 0.74±0.00c 40.31±0.55c 60 N.D. 39.73±0.05a 1.43±0.01b 0.40±0.01b 0.13±0.00d 0.65±0.01a 0.15±0.00d 1.37±0.01b 56.21±0.14a 70 N.D. 39.69±0.14a 1.47±0.01a 0.21±0.02c 0.15±0.00c 0.49±0.01b 0.20±0.00b 1.66±0.02a 57.88±0.39a 80 0.21±0.06b 37.54±0.19b N.D. N.D. 0.25±0.01b 0.39±0.03d 0.17±0.01c 1.71±005a 52.31±0.56ab 90 0.20±0.00b 35.74±0.08c N.D. N.D. 0.25±0.01b 0.40±0.00cd 0.15±0.01d 1.58±0.01a 46.32±0.25bc 100 0.41±0.11a 39.35±0.21a N.D. 0.47±0.06a 0.27±0.02a 0.43±0.04c 0.19±0.01b 1.65±0.04a 53.18±0.60ab
a-e Means(n=3) with different superscripts in a column of separate sample are significantly different
(p<0.05).f N.D.:Not detected
表 十五、不同成熟度圓金柑果皮與果肉甲醇萃取物之類黃酮組成
Table 15. Flavonoids composition in peel and pulp of Fortunella japonica Swingle (FJS) with different maturities Contents of kumquat( mg/100g dry weight dry weight)
Matuity
(%) Rutin Quercertin Kaempferol Hesperidin Hesperetin Naringin Naringenin Neohesperidin Diosmin
peel
30 N.D.e 25.40±1.62b 3.68±0.18bc 65.51±0.70a 1.26±0.06ab 10.88±0.19a 4.30±1.03a 26.27±0.25a 3.69±0.30c 50 N.D. 20.67±0.55c 3.25±0.37c 52.44±1.26b 0.45±0.02c 9.91±2.56ab 2.56±0.10b 25.44±1.48ab 6.36±1.28b 70 N.D. 20.43±0.69c 2.88±0.21c 26.31±0.56d 0.73±0.01c 3.75±1.52c 1.78±0.12bc 20.58±0.92c 7.90±0.98a 100 N.D. 42.35±0.97a 4.58±0.0a 44.81±0.43c 1.90±0.33a 8.42±0.27b 1.39±0.06c 24.09±0.49d 3.84±0.61c
pulp
30 N.D. 16.15±2.04ab N.D 7.41±0.41a N.D. 0.59±0.02c 0.32±0.02ab 4.29±0.15d 3.88±0.18a 50 N.D. 14.22±1.00b N.D 2.57±0.13c N.D. 0.53±0.03c 0.24±0.00b 32.74±0.38c 0.10±0.25c 70 N.D. 15.02±0.14ab N.D 3.77±0.14b N.D. 1.00±0.13a 0.36±0.00a 45.64±0.54b 1.85±0.40b 100 N.D. 16.24±1.98a N.D 4.01±0.02d N.D. 0.80±0.00b 0.26±0.03b 49.80±1.60a 1.51±0.39b
a-d Means(n=3) with different superscripts in a column of separate sample are significantly different (p<0.05).
e N.D.:Not detected
表 十六、不同成熟度長實金柑果皮甲醇萃取物之類黃酮成分變化與抗 氧化活性之相關性
Table 16. Pearson correlation coefficients between antioxidative activities and flavonoids in methanolic extract of peel of FMS with
different maturities
Scavenging effects on DPPH radical
Reducing power
Chealting effects on Fe+2
Rutin 0.8031** 0.8509*** 0.7978**
Naringin 0.1136 0.4236 0.2762 Hesperidin 0.8449*** 0.6423* 0.8758***
Diosmin 0.4276 0.4693 0.0939 Neohesperidin 0.7754** 0.8707*** 0.6678*
Quercertin 0.4699 0.5791* 0.1559
Naringenin 0.0762 0.3541 0.4159 Hesperetin 0.7396** 0.5008 0.9185***
Kaempferol 0.7080** 0.7633** 0.7794**
* : ( p< 0.05); **: ( p< 0.01); ***: (p< 0.001)
表 十七、不同成熟度長實金柑果肉甲醇萃取物之類黃酮成分變化與抗 氧化活性之相關性
Table 17. Pearson correlation coefficients between antioxidative activities and flavonoids in methanolic extract of pulp of FMS with different maturities
Scavenging effects on DPPH radical
Reducing power
Chealting effects on Fe+2
Rutin 0.6852* 0.9411*** 0.1304
Naringin 0.010 0.2934 0.4670 Hesperidin 0.6172* 0.3079 0.4382 Diosmin 0.6573* 0.1634 0.4909 Neohesperidin 0.7208** 0.6049* 0.020
Quercertin 0.7515** 0.2921 0.4901 Naringenin 0.6343* 0.5523 0.0640
Hesperetin 0.5825* 0.8829*** 0.2460 Kaempferol 0.3953 0.7979** 0.2968
* : ( p< 0.05); **: ( p< 0.01); ***: (p< 0.001)
表 十八、不同成熟度圓金柑果皮甲醇萃取物之類黃酮成分變化與抗氧 化活性之相關性
Table 18. Pearson correlation coefficients between antioxidative activities and flavonoids in methanolic extract of peel of FJS with
different maturities
Scavenging effects on DPPH radical
Reducing power
Chealting effects on Fe+2
Rutin -1 - -
Naringin 0.4488 0.9075** 0.1091 Hesperidin 0.4488 0.8111* 0.2514 Diosmin 0.7095* 0.8576** 0.3904 Neohesperidin 0.4589 0.8899** 0.1487
Quercertin 0.1873 0.5556 0.9347***
Naringenin 0.7370* 0.3082 0.6910 Hesperetin 0.4940 0.4947 0.7933*
Kaempferol 0.3262 0.7523* 0.8164*
1 Correlation coefficients are not exist because of the not detected flavonoids.
* : ( p< 0.05); **: ( p< 0.01); ***: (p< 0.001)
表 十九、不同成熟度圓金柑果肉甲醇萃取物之類黃酮成分變化與抗氧 化活性之相關性
Table 19. Pearson correlation coefficients between antioxidative activities and flavonoids in methanolic extract of pulp of FJS with
different maturities
Scavenging effects on DPPH radical
Reducing power
Chealting effects on Fe+2
Rutin -1 - -
Naringin 0.3961 0.2596 0.6013 Hesperidin 0.8029* 0.3159 0.1292
Diosmin 0.6952 0.4277 0.3114 Neohesperidin 0.5731 0.3843 0.0316
Quercertin 0.8515** 0.2488 0.2112 Naringenin 0.1183 0.8415** 0.9211
Hesperetin - - -
Kaempferol - - -
1 Correlation coefficients are not exist because of not detected flavonoids.
* : ( p< 0.05); **: ( p< 0.01); ***: (p< 0.001)
表 二十、長實金柑蜜餞加工過程中類黃酮含量之變化(mg/100g dry weight)
Table 20. Changes of flavonoids contents in FMS during processing for preserved fruit.
Rutin Naringin Hesperidin Diosmin Neohesperidin Quercertin Naringenin Hesperetin Kaempferol Fresh fruit 0.64±0.01a 0.31±0.01a 3.29±0.02a 44.59±1.39a 1.67±0.15a 31.50±0.15a 0.19±0.00a 0.01a±0.00 1.69±0.24a Salting 0.47±0.05b 0.26±0.00b 0.10±0.01b 35.80±0.14b 1.09±0.02b 29.62±0.01b 0.15±0.00b 0.00a±0.00 1.68±0.02a Washing 0.10±0.06c 0.21±0.01c 0.04±0.01c 33.13±0.06c 1.09±0.02b 27.86±0.02c N.D N.D N.D Blanching 0.01±0.00d 0.15±0.02d N.D 32.27±1.99c 1.03±0.06b 27.57±0.01c N.D N.D N.D Sugaring N.D.e N.D N.D 11.07±0.57d N.D 19.31±0.22d N.D N.D N.D
Drying N.D N.D N.D 11.69±0.13d N.D 19.49±0.02d N.D N.D N.D
a-d Means(n=3) with different superscripts in a column are significantly different (p<0.05).
e N.D.:Not detected