3-3-8 生化物質對抗體感測的干擾研究

人體血液中，除了血球、抗體與抗原之外，存在許多重要而微量 的生化物質。本研究選定了一些生化物質，依據人體內所含的平均濃 度，探討這些生化物質對於已建立的感測系統偵測抗體時是否會造成 影響。

這些在人體血液中生化物質常見的有有葡萄糖(glucose)，濃度約 為 100 mg/dL；尿酸(uric acid)，濃度約為 8 mg/dL；半胱胺酸(cystein)，

濃度約為 2.4 mg/dL；酪胺酸(tyrosine)，濃度約為 3.62 mg/dL。探討 生化物質存在環境對感測抗體的頻率訊號有何影響，其實驗結果如圖 3-32 至圖 3-45 所示。

各頻道在不同生化物質干擾物存在下的偵測感應訊號比較於圖 3-36、圖 3-41、圖 3-46 中。由表 3-1、表 3-2 以及表 3-3 顯示生化 物質對各頻道的干擾係數(interfering factor，I)皆很小，對各抗體的選 擇係數(selectivity，S)皆達 90 %以上。

圖 3-32 葡萄糖（100 mg/dL）的存在對 C₆₀/anti-human IgG 抗體石英 壓電感測器偵測 IgG 抗體時頻率訊號的影響

Fig.3-32 Effect of glucose (100 mg/dL) on frequency responses of C60/anti-human IgG PZ sensor for IgG

Time / sec

0 500 1000 1500 2000 2500

Fr equ ency shift / Hz

-800 -600 -400 -200 0 200

IgG + glucose IgG

Glucose

圖 3-33 尿酸（8 mg/dL）的存在對 C₆₀/anti-human IgG 抗體石英壓電 感測器偵測 IgG 抗體時頻率訊號的影響

Fig.3-33 Effect of uric acid (8 mg/dL) on frequency responses of C60/anti-human IgG PZ sensor for IgG

Time / sec

0 500 1000 1500 2000 2500

Frequency shif t / Hz

-700 -600 -500 -400 -300 -200 -100 0 100

IgG + uric acid IgG

Uric acid

圖 3-34 半胱胺酸（2.4 mg/dL）的存在對 C₆₀/anti-human IgG 抗體石 英壓電感測器偵測 IgG 抗體時頻率訊號的影響

Fig.3-34 Effect of cystein (2.4 mg/dL) on frequency responses of C60/anti-human IgG PZ sensor for IgG

Time / sec

0 500 1000 1500 2000 2500

Frequ ency shift / Hz

-800 -600 -400 -200 0 200

IgG+ cystein IgG

Cystein

圖 3-35 酪胺酸（3.62 mg/dL）的存在對 C₆₀/anti-human IgG 抗體石英 壓電感測器偵測 IgG 抗體時頻率訊號的影響

Fig.3-35 Effect of tyrosine (3.62 mg/dL) on frequency responses of C60/anti-human IgG PZ sensor for IgG

Time / sec

0 200 400 600 800 1000 1200 1400 1600 1800

Frequency shif t / Hz

-800 -600 -400 -200 0 200

IgG + tyrosine IgG

Tyrosine

圖 3-36 各生化干擾物對 C₆₀/anti-human IgG 抗體石英壓電感測器對 偵測 IgG 抗體時的感應訊號

Fig.3-36 Frequency responses of IgG with various bio-interfering compounds with the C60/anti-human IgG coated PZ sensor

Interfering compound

none glucose uric acid cystein tyrosine

Fre quency shift / Hz

0 200 400 600 800

表 3-1 生化干擾物對 C₆₀/anti-human IgG 抗體石英壓電感測器對偵測 IgG 抗體的影響

Table 3-1 Interference of various bio-interfering compounds on the C60/anti-human IgG coated PZ sensor for human IgG

Interfering Concentration of Interfering Selectivity compound

interfering compound(mg/dL)

factor , I S(

%

)

Glucose 100 3.08

×

10^-2 96.92 Uric acid 8 6.17

×

10^-3 99.38 Cystein 2.4 9.10

×

10^-2 90.90 Tyrosine 3.62 8.49

×

10^-2 91.51

I : interfering factor of interfering species = The difference of frequency shift between the presence and absence of interfering compound divided by frequency shift of absence of interfering compound.

S : selectivity of human immunoglobulin = (1.0－I)×100 %

圖 3-37 葡萄糖（100 mg/dL）的存在對 C₆₀/anti-human IgA 抗體石英 壓電感測器偵測 IgA 抗體時頻率訊號的影響

Fig.3-37 Effect of glucose (100 mg/dL) on frequency responses of C60/anti-human IgA PZ sensor for IgA

Time / sec

0 500 1000 1500 2000 2500

F requ ency shi ft / Hz

-1400 -1200 -1000 -800 -600 -400 -200 0 200

IgA + glucose IgA

Glucose

圖 3-38 尿酸（8 mg/dL）的存在對 C₆₀/anti-human IgA 抗體石英壓電 感測器偵測 IgA 抗體時頻率訊號的影響

Fig.3-38 Effect of uric acid (8 mg/dL) on frequency responses of C60/anti-human IgA PZ sensor for IgA

Time / sec

0 500 1000 1500 2000 2500

F requ ency shi ft / Hz

-1600 -1400 -1200 -1000 -800 -600 -400 -200 0 200

IgA + uric acid IgA

Uric acid

圖 3-39 半胱胺酸（2.4 mg/dL）的存在對 C₆₀/anti-human IgA 抗體石 英壓電感測器偵測 IgA 抗體時頻率訊號的影響

Fig.3-39 Effect of cystein (2.4 mg/dL) on frequency responses of C60/anti-human IgA PZ sensor for IgA

Time / sec

0 500 1000 1500 2000 2500

F requ ency shi ft / Hz

-1400 -1200 -1000 -800 -600 -400 -200 0 200

IgA + cystein IgA

Cystein

圖 3-40 酪胺酸（3.62 mg/dL）的存在對 C₆₀/anti-human IgA 抗體石英 壓電感測器偵測 IgA 抗體時頻率訊號的影響

Fig.3-40 Effect of tyrosine (3.62 mg/dL) on frequency responses of C60/anti-human IgA PZ sensor for IgA

Time / sec

0 500 1000 1500 2000 2500

F requ ency shi ft / Hz

-1400 -1200 -1000 -800 -600 -400 -200 0 200

IgA + tyrosine IgA

Tyrosine

圖 3-41 各生化干擾物對 C₆₀/anti-human IgA 抗體石英壓電感測器對 偵測 IgA 抗體時的感應訊號

Fig.3-41 Frequency responses of IgA with various bio-interfering compounds with the C60/anti-human IgA coated PZ sensor

Interfering compound

none glucose uric acid cystein tyrosine

Fr eq ue nc y s h ift / Hz

0 200 400 600 800 1000 1200 1400 1600

表 3-2 生化干擾物對 C₆₀/anti-human IgA 抗體石英壓電感測器對偵測 IgA 抗體的影響

Table 3-2 Interference of various bio-interfering compounds on the C60/anti-human IgA coated PZ sensor for human IgA

Interfering Concentration of Interfering Selectivity compound

interfering compound(mg/dL)

factor , I S(

%

)

Glucose 100 9.52

×

10^-2 90.48 Uric acid 8 8.81

×

10^-2 91.90 Cystein 2.4 1.58

×

10^-2 98.42 Tyrosine 3.62 3.49

×

10^-2 96.51

I : interfering factor of interfering species = The difference of frequency shift between the presence and absence of interfering compound divided by frequency shift of absence of interfering compound.

S : selectivity of human immunoglobulin = (1.0－I)×100 %

圖 3-42 葡萄糖（100 mg/dL）的存在對 C₆₀/anti-human IgM 抗體石英 壓電感測器偵測 IgM 抗體時頻率訊號的影響

Fig.3-42 Effect of glucose (100 mg/dL) on frequency responses of C60/anti-human IgM PZ sensor for IgM

Time / sec

0 500 1000 1500 2000 2500

F requ ency shi ft / Hz

-6000 -5000 -4000 -3000 -2000 -1000 0 1000

IgM + glucose IgM

Glucose

圖 3-43 尿酸（8 mg/dL）的存在對 C₆₀/anti-human IgM 抗體石英壓電 感測器偵測 IgM 抗體時頻率訊號的影響

Fig.3-43 Effect of uric acid (8 mg/dL) on frequency responses of C60/anti-human IgM PZ sensor for IgM

Time / sec

0 500 1000 1500 2000 2500

F requ ency shi ft / Hz

-6000 -5000 -4000 -3000 -2000 -1000 0 1000

IgM + uric acid IgM

Uric acid

圖 3-44 半胱胺酸（2.4 mg/dL）的存在對 C₆₀/anti-human IgM 抗體石 英壓電感測器偵測 IgM 抗體時頻率訊號的影響

Fig.3-44 Effect of cystein (2.4 mg/dL) on frequency responses of C60/anti-human IgM PZ sensor for IgM

Time / sec

0 200 400 600 800 1000 1200 1400 1600 1800 2000

F requ ency shi ft / Hz

-6000 -5000 -4000 -3000 -2000 -1000 0 1000

IgM + cystein IgM

Cystein

圖 3-45 酪胺酸（3.62 mg/dL）的存在對 C₆₀/anti-human IgM 抗體石英 壓電感測器偵測 IgM 抗體時頻率訊號的影響

Fig.3-45 Effect of tyrosine (3.62 mg/dL) on frequency responses of C60/anti-human IgM PZ sensor for IgM

Time / sec

0 500 1000 1500 2000 2500

Fr e q u e n cy s h ift / Hz

-6000 -5000 -4000 -3000 -2000 -1000 0 1000

IgM + tyrosine IgM

Tyrosine

圖 3-46 各生化干擾物對 C₆₀/anti-human IgM 抗體石英壓電感測器對 偵測 IgM 抗體時的感應訊號

Fig.3-46 Frequency response of IgM with various bio-interfering compounds with the C60/anti-human IgM coated PZ sensor

Interfering compound

none glucose uric acid cystein tyrosine

Fr eq ue nc y s h ift / Hz

0 1000 2000 3000 4000 5000 6000

表 3-3 生化干擾物對 C₆₀/anti-human IgM 抗體石英壓電感測器對偵 測 IgM 抗體的影響

Table 3-3 Interference of various bio-interfering compounds on the C60/anti-human IgM coated PZ sensor for human IgM

Interfering Concentration of Interfering Selectivity compound

interfering compound(mg/dL)

factor , I S(

%

)

Glucose 100 4.83

×

10^-2 95.17 Uric acid 8 9.42

×

10^-2 90.58 Cystein 2.4 7.92

×

10^-2 92.08 Tyrosine 3.62 9.63

×

10^-2 90.37

I : interfering factor of interfering species = The difference of frequency shift between the presence and absence of interfering compound divided by frequency shift of absence of interfering compound.

S : selectivity of human immunoglobulin = (1.0－I)×100 %

在文檔中 3-1-1C 60 /PVC 塗佈壓電晶體對抗人體免疫球蛋白抗體感應訊 號 (頁 41-59)